Oncological patients' reactions to COVID-19 pandemic: A single institution prospective study.

peer reviewedBACKGROUND: The spread of the COVID-19 pandemic has led to a rapid reorganization in all human and hospital activities, with impact on cancer patients. AIM: An analysis of cancer patients fears, and awareness of COVID-19 has been done in this study. METHODS AND RESULTS: We analyzed cancer patients' reactions to the pandemic and their perception of oncological care reorganization, through a 12-item survey, proposed at the peak of pandemic and 3 months later. Overall, 237 patients were included in the study. During the peak of pandemic 34.6% of patients were more worried about COVID-19 than cancer versus 26.4% in the post-acute phase (p = .013). Although 49.8% of patients in the acute phase and 42.3% in the post-acute phase considered their risk of death if infected ≥50%, and more than 70% of patients thought to be at higher risk of complications, the majority of them did not consider the possibility to stop or delay their treatment. Patients were more interested in following news about COVID-19 than cancer and they complied with all preventive measures in more than 90% of the cases. CONCLUSIONS: Although cancer patients worried about COVID-19 and evaluated the risk of complication or death due to COVID-19 as extremely high, they were still asking for the best oncological treatment

Processus de gestion des essais cliniques à l'Agence française de sécurité sanitaire des produits de santé

PARIS-BIUP (751062107) / SudocSudocFranceF

Le Statut des Populations d’Ongulés de Taille Moyenne en 2010, Ranch de Gibier de Nazinga, Burkina Faso (Afrique de l’Ouest)

The present study consists of analyzing the raw data collected from the annual line-transect foot count of medium-sized ungulates, carried out at the Nazinga Game Ranch (NGR), Burkina Faso (Western Africa), in both 2001 and 2010. The annual census focused on the seven main medium-sized ungulates, namely (in alphabetical order), the Bushbuck (Tragelaphus scriptus), the Common Warthog (Phacochoerus africanus), the Defassa Waterbuck (Kobus ellipsiprymnus), the Grimm’s Duiker (Sylvicapra grimmia), the Oribi (Ourebia ourebi), the Roan Antelope (Hippotragus equinus) and the Western Hartebeest (Alcelaphus buselaphus). The census also focused on illegal anthropogenic activities. The wildlife populations were quantitatively analyzed through an estimation of their absolute density via the distance sampling method and through their evolution over the last decade using two relative density indexes, namely the Kilometric Abundance Index (KAI) and a spatial distribution index. These indexes were also used to measure the evolution of illegal activities over the same period. Both the wildlife observations and the anthropogenic observations were mapped using the Kernel method. Following an increasing trend in their population between 2001 and 2010, both the Roan Antelope and the Western Hartebeest reached an estimated density of 4.7 individuals per km², while the Defassa Waterbuck reached 2.4 individuals per km². Following an inverse trend over the same period, the Bushbuck, the Grimm’s Duiker and the Oribi reached an estimated density of 0.4 individuals per km². As for the Common Warthog, its estimated density of 2.5 individuals per km² seemed to remain unchanged during that decade. A comparison between wildlife observations and anthropogenic observations reveals a high decrease in animal densities in the north, east and west peripheral borders of the NGR and a flagrant extension of the proportion of the ranch being subjected to illegal activities (poaching, cattle herding, etc.)

Digital squamous cell carcinoma in dogs: epidemiological, histological and imunohistochemical study

International audienc

Implication of Netrin-1 Gain of Expression in Canine Nodal Lymphoma

Netrin-1 is a member of the laminin superfamily, and is known to interact with specific receptors, called dependence receptors. While upon netrin-1 binding these receptors initiate positive signaling, in absence of netrin-1, these receptors trigger apoptosis. Tumor cells can avoid apoptosis by inactivating these receptors or by gaining ligand expression. The aim of the present study was to investigate the expression of netrin-1, the ligand of dependence receptors, in canine healthy lymph nodes (LN), and in lymphomas and to evaluate efficiency of a netrin-1 interfering compound in cell cultures from canine lymphoma. Thirty-two control LN and 169 lymphomas were analyzed through immunohistochemistry. Netrin-1 was expressed in the nucleoli of lymphoid and non-lymphoid cells in controls. Acquisition of a cytoplasmic expression was present in B-cell lymphomas (23.1 % in low-grade and 50.6% in high-grade) and T-cell lymphomas (50.0 % in low-grade and 78.8 % in high-grade), with a significant difference between the high- and low-grade in B-cell lymphomas. Through flow cytometry, we showed a significant increase in netrin-1 expression in either high-grade B-cell and T-cell lymphomas (19 and 5, respectively) compared with healthy LN (5), likewise an RT-qPCR analysis demonstrated a significant increase in netrin-1 expression level in 14 samples of lymphomas compared with eight samples of healthy LN. A T-cell aggressive canine lymphoma cell line and four primary canine nodal lymphomas cell cultures were treated with a netrin-1 interfering antibody. Apoptosis by measuring caspase 3 activity was significantly increased in the cell line and viability was decreased in three of the four primary cell cultures. Together, these data suggest that netrin-1 expression is increased in lymphoma, and more specifically in high-grade lymphomas, and that netrin-1 can act as a survival factor for the neoplastic cells, and so be a therapeutic target

Penetration of ciprofloxacin into human pleural fluid

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Allégories de l’estomac au xixe siècle

Proposer un panorama des virtualités allégoriques de l’estomac au xixe siècle : tel est l’objectif de cet ouvrage collectif qui, à l’opposé d’une exhaustivité potentiellement redondante, fait le pari de l’ouverture suscitée par des approches différenciées. Les contributions de chercheurs en littérature, philosophie, histoire et histoire de l’art mettent en lumière ce que l’estomac pouvait incarner et pourquoi il pouvait constituer un trope privilégié pour dire le xixe siècle. De cette enquête ressort la profonde ambivalence axiologique de l’estomac, mais aussi sa « plasticité argumentative », caractéristique, selon Judith Schlanger, des représentations organicistes. Emblème de la caricature et support de la satire, l’estomac est également une figure clé dans la formulation d’un système de pensée ou dans l’élaboration d’une poétique, au point d’apparaître comme une figure caractéristique d’un siècle que l’on qualifie volontiers de matérialiste

Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology

International audienceDogs with lymphoma are established as good model for human non-Hodgkin lymphoma studies. Canine cell lines derived from lymphomas may be valuable tools for testing new therapeutic drugs. In this context, we established a canine T-cell line, PER-VAS, from a primary aggressive T-cell lymphoma with large granular morphology. Flow cytometric analysis revealed a stable immunophenotype: PER-VAS cells were positively labelled for CD5, CD45, MHC II and TLR3, and were negative for CD3, CD4 and CD8 expression. Although unstable along the culture process, IL-17 and MMP12 proteins were detectable as late as at passages 280 and 325i.e. respectively 24 and 29 months post isolation. At passage 325, PER-VAS cells maintained the expression of IL-17, CD3, CD56, IFNgamma and TNFalpha mRNAs as shown by RT-PCR analysis. Stable rearrangement of the TCRgamma gene has been evidenced by PCR. PER-VAS cells have a high proliferation index with a doubling time of 16.5h and were tumorigenic in Nude mice. Compared to the canine cell lines already reported, PER-VAS cells display an original expression pattern, close to NKT cells, which makes them valuable tools for in vitro comparative research on lymphomas