Pictures taken at day 60 from sibling juvenile males (in rows) that had experienced different nutritional conditions (left: high quality food; right: standard food) from day 35 until day 60, i.e. immediately after nutritional dependence from the parents.

<p>Pictures taken at day 60 from sibling juvenile males (in rows) that had experienced different nutritional conditions (left: high quality food; right: standard food) from day 35 until day 60, i.e. immediately after nutritional dependence from the parents.</p

Number of 10 s intervals (means±SE) spent by females perching near the males that had received different nutritional conditions from day 35 to day 60.

<p>Number of 10 s intervals (means±SE) spent by females perching near the males that had received different nutritional conditions from day 35 to day 60.</p

Differences in size of the orange cheek patch at day 60 of paired males as used in mate choice tests at 6 month of age.

<p>Differences in size of the orange cheek patch at day 60 of paired males as used in mate choice tests at 6 month of age.</p

Experimental setup for periods of food manipulation and mate choice experiments (a) period of experimental food manipulation.

<p>Offspring were kept in the adjacent aviaries with one adult male as song tutor centered between the aviaries. The front of each aviary was made of mesh wire, the other parts of plywood. (1) Offspring aviaries had a perch in the rear (4) and a perch in front of the tutor (5). The separation between the two aviaries had mesh wire (2) instead of plywood between the frontal perch (5) and the front panel. Thus, offspring of the two groups could be in visual and acoustic contact to each other. Food and water was provided in dishes and differed in adjacent aviaries according to the two nutritional treatments. Overall nine of such setups were used, all located in the same experimental room. (b) mate choice experiments. Females could move freely in the large free-flight aviary. Two cages (2) with males that came from two adjacent aviaries (as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000901#pone-0000901-g001" target="_blank">Figure 1a</a>) were placed in the top rear corners of the aviaries. Females were provided with food and water (1) and perches in a neutral zone (3). Perches of male cages extended into the female cage (4) so that females could perch directly in front of either male (preference zone) without being able to see the other male. Males could not see each other due to a visual barrier between cages. Drawings are partly to scale. See text for further details</p

Videoplayback_responsedata_and_independentvariables

This data file includes three data sheets and one legend sheet. The first data sheet ("sociability") contains the primary data for all the experimental videoplayback trials. The second data sheet ("control") contains all the data for the control trials. The third data sheet ("firstlast") contains all the data, specifically for the first and last choice analysis of the experimental trials. The legend sheet provides information on all the column names used in the three data sheets of this file

Snijders etal 2016/2017 Ecology and Evolution Data

This file includes all the data used for the statistical analyses in the manuscript. The data include information on the experimental stimuli, the subject's individual traits, vocal response, spatial response, and the neighborhood response (3 sheets). An additional sheet provides the legend for the column names

Mean ± SE percentage of their songs that males used to overlap playback songs, in males that later in the season were paired (n = 7), and in males that remained unpaired throughout the breeding season (bachelors; n = 14).

<p>Mean ± SE percentage of their songs that males used to overlap playback songs, in males that later in the season were paired (n = 7), and in males that remained unpaired throughout the breeding season (bachelors; n = 14).</p

Effect of playback treatment and playback period on mean ± SE song output (a) and on structural song parameters (b) by male nightingales.

<p>One group of nocturnally singing males (n = 14) received a playback from the same height as their own song perch (‘same level’), the other group (n = 13) received a playback from 3 metres higher than their own song perch (‘high’). High scores on PC1 (a) indicate high song rates but short pause durations and low numbers and durations of interruptions (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032194#pone-0032194-t001" target="_blank">Table 1</a>). High scores on PC2 (b) indicate long song lengths and high percentages of songs with rapid broadband trills and initial whistles.</p

Effects of the nutritional treatments on resting metabolic rate.

<p>Mean (±SE) resting metabolic rate (RMR) for subjects from the different early nutritional treatments, LH, HL and HH. In the LH (low-high) treatment subjects fed on a low quality seed diet until day 17 (nestling period) followed by a protein enriched high quality diet up to day 35 (fledging period). Subjects in the HL (high-low) treatment experienced the nutritional diets vice versa. In the HH treatment (high-high) a high quality protein enriched diet was fed throughout the nestling and fledgling phase (see text for details).</p

Principle component analysis on seven nightingale song parameters, showing unrotated component loadings.

<p>PC1 represents song output parameters, and PC2 represents structural song parameters. Loadings of variables that made an important contribution to the components are indicated in bold. High scores on PC1 indicate high song rates but short durations of pauses and of song interruptions, and low numbers of interruptions; high scores on PC2 are mainly related to long song lengths and high percentage of songs with trills and initial whistles.</p