Quantification of atmospheric n2 fixed by cowpea, pigeonpea and greengram grown on ferralsols in Muheza District, Tanzania

Tanzania Journal of Agricultural Sciences 2013, Vol. 10(1) : 29-37Legume crops are usually intercropped with cereals in small-scale farming systems in Tanzania. This aims at taking advantage of legumes to replenish soil nitrogen (N due to legumes’ N2 fixation). Glasshouse pot experiments and field trials were carried out atMlingano Agricultural Research Institute in Tanga, Tanzania, with the objective to find out how much N2 can be fixed by cowpea, pigeonpea and greengram using native Rhizobia, thus substituting for the use of N-mineral fertilizers in maize production on Ferralsols. In the glasshouse pot experiment, the legumes were grown in 5L plastic pots for 40 days in soil sampled from 0 – 20 cm layer. The field experiment was maize – legumes intercropping whereby the N2 fixation assessment was done 35 days after planting. In both cases, maize (Katumani variety) was the non- N2- fixing reference crop. The legumes formed effective nodules with the native Rhizobia. Different quantities of N2 gas were fixed by the different legumes, the differences being attributed to the legumes’ differences in their genetic characteristics. In the field, monocropped cowpea, pigeonpea and greengram fixed 38, 21, and 49 kg N2, respectively. In the intercrop system cowpea, pigeonpea and greengram fixed 16, 4, and 24 kg N2, respectively. In both cases, the quantities of N2 fixed were less than 50% of their total N accumulation. It was concluded that in Muheza, maize grown in association with the legumes would continue to need supplementation of mineral N fertilizer to maximize its yields

Contribution of legume rotations to the nitrogen requirements of a subsequent maize crop on a rhodic ferralsol in Tanga, Tanzania

Tanzania Journal of Agricultural Sciences 2013, Vol. 12(1) : 23-29Industrial fertilizers are expensive for small-scale farmers who, as alternative, rely on legume crops for providing N for a subsequent maize crop. A legume-maize rotational experiment was carried out on a Rhodic Ferralsol at Mlingano Agricultural Research Institute in Muheza, Tanga, Tanzania, to evaluate the effects of legumes rotation in meeting the N fertilizer requirements of maize. The experimental site was located at 39o 52’E, 5o 10’S and 183 metres above sea level (m.a.s.l.). The experiment was conducted for two rotation cycles whereby cowpea, pigeonpea or greengram were grown during the short rains followed by maize during the long rains. The maize rotations were imposed on plots on which legumes had been grown during the previous legume rotation. Monoculture maize was grown with treatments of 0, 25, 50 and 100 kg N ha-1 imposed for purposes of plotting N fertilizer response curves. Based on the response curve lines, the effects of the legume rotation on maize yields were compared and translated as N fertilizer equivalency of the legumes in question. The grain and residue yields of the three legumes were significantly different (P<0.01), a fact which was attributed to the genetic differences of the legume species. The maize yields following rotation with each of the three legumes were significantly higher (P<0.05) than those under continuous maize. The effects of the rotations on increasing the maize yields were equivalent to application of 25, 19 and 16 kg N ha-1 for the cowpea, pigeonpea and greengram rotations, respectively. It was, however, concluded that the contributions of N by the legumes in the legume-maize rotations were not enough to satisfy the maize N requirements of 50 kg N/ha; hence supplementation with mineral N, in addition to the rotations, is necessary for increased yields

Contribution of legume rotations to the nitrogen requirements of a subsequent maize crop on a rhodic ferralsol in Tanga, Tanzania

Tanzania Journal of Agricultural Sciences 2013, Vol. 12(1) : 23-29Industrial fertilizers are expensive for small-scale farmers who, as alternative, rely on legume crops for providing N for a subsequent maize crop. A legume-maize rotational experiment was carried out on a Rhodic Ferralsol at Mlingano Agricultural Research Institute in Muheza, Tanga, Tanzania, to evaluate the effects of legumes rotation in meeting the N fertilizer requirements of maize. The experimental site was located at 39o 52’E, 5o 10’S and 183 metres above sea level (m.a.s.l.). The experiment was conducted for two rotation cycles whereby cowpea, pigeonpea or greengram were grown during the short rains followed by maize during the long rains. The maize rotations were imposed on plots on which legumes had been grown during the previous legume rotation. Monoculture maize was grown with treatments of 0, 25, 50 and 100 kg N ha-1 imposed for purposes of plotting N fertilizer response curves. Based on the response curve lines, the effects of the legume rotation on maize yields were compared and translated as N fertilizer equivalency of the legumes in question. The grain and residue yields of the three legumes were significantly different (P<0.01), a fact which was attributed to the genetic differences of the legume species. The maize yields following rotation with each of the three legumes were significantly higher (P<0.05) than those under continuous maize. The effects of the rotations on increasing the maize yields were equivalent to application of 25, 19 and 16 kg N ha-1 for the cowpea, pigeonpea and greengram rotations, respectively. It was, however, concluded that the contributions of N by the legumes in the legume-maize rotations were not enough to satisfy the maize N requirements of 50 kg N/ha; hence supplementation with mineral N, in addition to the rotations, is necessary for increased yields

Facility-based disease surveillance and Bayesian hierarchical modeling to estimate endemic typhoid fever incidence, Kilimanjaro Region, Tanzania, 2007-2018.

Growing evidence suggests considerable variation in endemic typhoid fever incidence at some locations over time, yet few settings have multi-year incidence estimates to inform typhoid control measures. We sought to describe a decade of typhoid fever incidence in the Kilimanjaro Region of Tanzania. Cases of blood culture confirmed typhoid were identified among febrile patients at two sentinel hospitals during three study periods: 2007-08, 2011-14, and 2016-18. To account for under-ascertainment at sentinel facilities, we derived adjustment multipliers from healthcare utilization surveys done in the hospital catchment area. Incidence estimates and credible intervals (CrI) were derived using a Bayesian hierarchical incidence model that incorporated uncertainty of our observed typhoid fever prevalence, of healthcare seeking adjustment multipliers, and of blood culture diagnostic sensitivity. Among 3,556 total participants, 50 typhoid fever cases were identified. Of typhoid cases, 26 (52%) were male and the median (range) age was 22 (<1-60) years; 4 (8%) were aged <5 years and 10 (20%) were aged 5 to 14 years. Annual typhoid fever incidence was estimated as 61.5 (95% CrI 14.9-181.9), 6.5 (95% CrI 1.4-20.4), and 4.0 (95% CrI 0.6-13.9) per 100,000 persons in 2007-08, 2011-14, and 2016-18, respectively. There were no deaths among typhoid cases. We estimated moderate typhoid incidence (≥10 per 100 000) in 2007-08 and low (<10 per 100 000) incidence during later surveillance periods, but with overlapping credible intervals across study periods. Although consistent with falling typhoid incidence, we interpret this as showing substantial variation over the study periods. Given potential variation, multi-year surveillance may be warranted in locations making decisions about typhoid conjugate vaccine introduction and other control measures

New advances in CMV and immunosenescence

Immunosenescence, defined as the age-associated dysregulation and dysfunction of the immune system, is characterized by impaired protective immunity and decreased efficacy of vaccines. An increasing number of immunological, clinical and epidemiological studies suggest that persistent Cytomegalovirus (CMV) infection is associated with accelerated aging of the immune system and with several age-related diseases. However, current evidence on whether and how human CMV (HCMV) infection is implicated in immunosenescence and in age-related diseases remains incomplete and many aspects of CMV involvement in immune aging remain controversial. The attendees of the 4th International Workshop on “CMV & Immunosenescence”, held in Parma, Italy, 25–27th March, 2013, presented and discussed data related to these open questions, which are reported in this commentary

Inflammaging decreases adaptive and innate immune responses in mice and humans

Both the innate and adaptive immune systems decline with age, causing greater susceptibility to infectious diseases and reduced responses to vaccination. Diseases are more severe in elderly than in young individuals and have a greater impact on health outcomes such as morbidity, disability and mortality. Aging is characterized by increased low-grade chronic inflammation, called “inflammaging”, measured by circulating levels of TNF-α, IL-6 and CRP, as well as by latent infections with viruses such as cytomegalovirus (CMV). Inflammaging has received considerable attention because it proposes a link between changes in immune cells and a number of diseases and syndromes typical of old age. In this review we aim at summarizing the current knowledge on pathways contributing to inflammaging, on immune responses down-regulated by inflammation and mechanisms proposed. The defects in the immune response of elderly individuals presented in this review should help to discover avenues for effective intervention to promote healthy aging