A differentiated multi-loops bath recirculation system for precision machine tools

Traditional bath recirculation cooler for precision machine tools always has the uniform and open-loop cooling strategy onto different heat generating parts. This causes redundant generated heat being transferred into the machine structure, and results in unsatisfactory thermal errors of precision machine tools. For the solution of this problem, this paper presents the differentiated multi-loops bath recirculation system. The developed system can accomplish differentiated and close-loop cooling strategies onto machine heat generating parts during its operation. Specially, in order to illustrate the advantages of this system, constant supply cooling powers strategy is presented with its applications onto a certain type of built-in motorized spindle. Consequently, advantages of the proposed strategy based on the differentiated multi-loops bath recirculation system are verified experimentally in the environment within consistent temperature (TR = 20 ± 0.3°C). Compared with room temperature tracing strategy based on the traditional bath recirculation cooler, the constant supply cooling powers strategy is verified to be advantageous in spindle temperature stabilization and thermal errors decrease

A tandem array of ent-kaurene synthases in maize with roles in gibberellin and more specialized metabolism

While most commonly associated with its role in gibberellin (GA) phytohormone biosynthesis, ent-kaurene also serves as an intermediate in more specialized diterpenoid metabolism, as exemplified by the more than 800 known derived natural products. Among these are the maize kauralexins. However, no ent-kaurene synthases (KSs) have been identified from maize. The maize GA-deficient dwarf-5 (d5) mutant has been associated with a loss of KS activity. The relevant genetic lesion has been previously mapped, and was found here to correlate with the location of the KS-like gene ZmKSL3. Intriguingly, this forms part of a tandem array with two other terpene synthases (TPSs). Although one of these, ZmTPS1, has been previously reported to encode a sesquiterpene synthase, and both ZmTPS1 and that encoded by the third gene, ZmKSL5, have lost the N-terminal γ-domain prototypically associated with KS(L)s, all three genes fall within the KS(L) or TPS-e sub-family. Here it is reported that all three genes encode enzymes that are targeted to the plastid in planta, where diterpenoid biosynthesis is initiated, and which all readily catalyze the production of ent-kaurene. Consistent with the closer phylogenetic relationship of ZmKSL3 with previously identified KSs from cereals, only transcription of this gene is affected in d5 plants. On the other hand, the expression of all three of these genes is inducible, suggesting a role in more specialized metabolism, such as that of the kauralexins. Thus, these results clarify not only gibberellin phytohormone, but also diterpenoid phytoalexin biosynthesis in this important cereal crop plant

Characterization of CYP71Z18 indicates a role in maize zealexin biosynthesis

Maize (Zea mays) produces zealexins as phytoalexins, with the inducible production of these antibiotics providing biochemical protection against fungal infection. However, the biosynthesis of these sesquiterpenoids has remained unclear. In particular, it is unclear how the olefinic precursor, (S)-β-macrocarpene produced by the characterized maize sesquiterpene synthases TPS6/11, is further elaborated to form the bioactive zealexins. The first step is likely to be conversion of carbon-15 (C15) from a methyl group to a carboxylic acid by a cytochrome P450 mono-oxygenase (CYP). In this study, CYP71Z18, whose transcription is strongly induced by fungal infection, was found to catalyze oxidation of C15 in (S)-β-macrocarpene, forming zealexin A1. The inducible transcription of CYP71Z18 matches that observed for TPS6/11 and the accumulation of zealexins, which is consistent with a role for CYP71Z18 in sesquiterpenoid phytoalexin production. This completes identification of zealexin A1 biosynthesis, and represents the initial CYP identified for the production of maize terpenoid phytoalexins

The Redox-Sensing Regulator Rex Contributes to the Virulence and Oxidative Stress Response of Streptococcus suis Serotype 2

Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen responsible for septicemia and meningitis. The redox-sensing regulator Rex has been reported to play critical roles in the metabolism regulation, oxidative stress response, and virulence of various pathogens. In this study, we identified and characterized a Rex ortholog in the SS2 virulent strain SS2-1 that is involved in bacterial pathogenicity and stress environment susceptibility. Our data show that the Rex-knockout mutant strain Δrex exhibited impaired growth in medium with hydrogen peroxide or a low pH compared with the wildtype strain SS2-1 and the complementary strain CΔrex. In addition, Δrex showed a decreased level of survival in whole blood and in RAW264.7 macrophages. Further analyses revealed that Rex deficiency significantly attenuated bacterial virulence in an animal model. A comparative proteome analysis found that the expression levels of several proteins involved in virulence and oxidative stress were significantly different in Δrex compared with SS2-1. Electrophoretic mobility shift assays revealed that recombinant Rex specifically bound to the promoters of target genes in a manner that was modulated by NADH and NAD+. Taken together, our data suggest that Rex plays critical roles in the virulence and oxidative stress response of SS2

Chemokine (C-C Motif) Ligand 2 (CCL2) in Sera of Patients with Type 1 Diabetes and Diabetic Complications

Chemokine (C-C motif) ligand 2 (CCL2), commonly known as monocyte chemoattractant protein-1 (MCP-1), has been implicated in the pathogenesis of many diseases characterized by monocytic infiltration. However, limited data have been reported on MCP-1 in type 1 diabetes (T1D) and the findings are inconclusive and inconsistent.In this study, MCP-1 was measured in the sera from 2,472 T1D patients and 2,654 healthy controls using a Luminex assay. The rs1024611 SNP in the promoter region of MCP-1 was genotyped for a subset of subjects (1764 T1D patients and 1323 controls) using the TaqMan-assay.Subject age, sex or genotypes of MCP-1 rs1024611SNP did not have a major impact on serum MCP-1 levels in either healthy controls or patients. While hemoglobin A1c levels did not have a major influence on serum MCP-1 levels, the mean serum MCP-1 levels are significantly higher in patients with multiple complications (mean = 242 ng/ml) compared to patients without any complications (mean = 201 ng/ml) (p = 3.5×10(-6)). Furthermore, mean serum MCP-1 is higher in controls (mean = 261 ng/ml) than T1D patients (mean = 208 ng/ml) (p<10(-23)). More importantly, the frequency of subjects with extremely high levels (>99(th) percentile of patients or 955 ng/ml) of serum MCP-1 is significantly lower in the T1D group compared to the control group (odds ratio = 0.11, p<10(-33)).MCP-1 may have a dual role in T1D and its complications. While very high levels of serum MCP-1 may be protective against the development of T1D, complications are associated with higher serum MCP-1 levels within the T1D group

Demodulation Technique Based on Laser Interference for Weak Photo-Acoustic Signals on Water Surface

To detect underwater sound-generating targets, a water surface acoustic wave laser interference and signal demodulation technique is proposed in this paper. The underlying principle of this technique involves casting a laser beam onto the water surface disturbed by an underwater acoustic source and creating interference between lights reflected by the surface and reference lights. A data acquisition and processing system was employed to obtain water surface acoustic wave information from the interference signals by means of demodulation, thus allowing detection of the underwater target. For the purpose of this study, an interference detection platform was set up in an optical dark chamber. High-frequency water surface fluctuations were introduced in the reference optical path as the phase generated carriers to create laser interference signals in two different paths, which received demodulation based on an improved arc tangent demodulation algorithm and characteristic ratio algorithm, respectively, in view of their different frequencies. Water surface wave information was then derived from such low-frequency and high-frequency signals. According to test results, in the frequency range of 200 Hz&#8315;10 kHz, the frequency detection accuracy was better than 1 Hz. The amplitude measurements exhibited high repeatability, with a standard deviation lower than 2.5 nm. The theory proposed in this paper is therefore experimentally verified with good results