µXANES spectra and corresponding fitted pre-edge peaks. Earliest microbial trace fossils in Archaean pillow lavas under scrutiny: new micro-X-ray absorption near-edge spectroscopy, metamorphic and morphological constraints

Representative µXANES spectra and corresponding fitted pre-edge peaks for all spot analyses along line scan 1 (cf. Fig. 3 main paper for map position

Additional file 4: of Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans

Breakdown of the image analysis protocol for green images. (XLSX 33 kb

Additional file 9: of Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans

Breakdown of CellProfiler protocol for green and red images. (DOCX 128 kb

Additional file 5: of Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans

Raw data of Phsp-6::GFP reporter measurements. Gene-by-gene data of the fold change (FC) and adjusted P value (adj.pvalue) obtained after data analysis. RNAi clones appear ordered by their position in the OrthoList RNAi sublibrary, with the GenePair name as well as the common gene name. In sheet 2 is the list of genes already described to regulate the UPRmt in previous screens. (XLSX 102 kb

Additional file 6: of Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans

Raw data of size measurements. Gene-by-gene data of the fold change (FC) and adjusted P value (adj.pvalue) obtained after data analysis. RNAi clones appear ordered by their position in the OrthoList RNAi sublibrary, with the GenePair name as well as the common gene name. (XLSX 112 kb

Additional file 3: of Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans

Detailed description of the protocol. Report of the strain and reagents used, in addition to a more exhaustive explanation in the methodology followed in the RNAi study. (DOCX 21 kb

Additional file 7: of Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans

Figure S2. Outline of the combined green/red image analysis for balanced mutants. Briefly, post well segmentation in the green channel, the worms are segmented after enhancing the contrast in the Brightfield channel (image 1). Acceptance criteria (Additional File 8) are applied to remove artefacts (blue accepted, yellow rejected). The worm mask is transferred to the green image to identify, if needed, worms with green heads based on the green head ID (image 2 and image 3). With the final worm mask dilated (image 5), the dilated region around the worm is used to calculate the immediate background intensity. Finally, the targets are linked together to get all three measurement regions (Dilated worms, Worm minus Green head and Final worm mask) together in one target (Fig. 4). As mentioned earlier, the software links targets, two at a time, and there must be at least one pixel overlap to achieve a linkage. (PDF 7627 kb

Additional file 1: of Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans

Figure S1. Effect of haf-1 deletion in the UPRmt. a. haf-1(ok705) deletion further induces Phsp-6::GFP expression upon depletion of the PHB complex (phb-1(RNAi) or phb-2(RNAi)). Bar graphs show quantification of Phsp-6::GFP. Day 1 adults are shown. b. haf-1(ok705) deletion also enhances the expression of the UPRmt reporter Phsp-6::GFP in phb-2(tm2998) deletion mutants. Day 5 adults were imaged. c. haf-1(ok705) deletion further induces Phsp-6::GFP expression upon RNAi depletion of the mitochondrial AAA protease (spg-7(RNAi)). Day 1 adults are shown. All bar graphs show quantification of Phsp-6::GFP (mean ± SD); P value shown in each panel; two-tailed unpaired t test; n = 20; two biological repeats, one representative experiment is shown. (PDF 3830 kb

Marginal means (adjusted prevalence) for significant gender*city interactions in lifetime drinkers and people with AUD in the past 12 months.

<p>Note: No women in Madrid met the criteria for past 12-month AUD.</p

Past 12-month and lifetime alcohol use patterns (use of alcohol, and consumption indicator: Index^*) in the overall sample and the subsample of lifetime drinkers (people who reported drinking any type of alcoholic beverage at least 12 times during their lifetime).

<p>Past 12-month and lifetime alcohol use patterns (use of alcohol, and consumption indicator: Index^{<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196574#t002fn002" target="_blank">*</a>}) in the overall sample and the subsample of lifetime drinkers (people who reported drinking any type of alcoholic beverage at least 12 times during their lifetime).</p