1 research outputs found
IMMUNOMODULATORY AND GROWTH-PROMOTING POTENTIAL OF LOWCOST PROBIOTIC PRODUCT IN Penaeus monodon CULTURE SYSTEM
Sposobnost probiotičkog proizvoda s niskim troškovima
procijenjena je pri uzgoju vrste Penaeus monodon u
vanjskom sustavu uzgoja tijekom 90 dana. Probiotski
proizvod niske cijene formuliran je miješanjem bujonske
kulture Bacillus cereus zajedno s komercijalnom praškastom
kredom u omjeru 1: 1. Pripremljeni probiotički produkt uz
dodatak praha inkorporiran je zajedno s hranom za rakove
u obroke različite koncentracije (1 do 5%). Održivost B.
cereusa u eksperimentalnoj prehrani testirana je jednom u
30 dana do 90 dana tijekom trajanja eksperimenta. Nakon
90 dana od početka hranidbenog istraživanja, određene
su performanse rasta rakova. Utvrđena je maksimalna
proizvodnja od 11,98 ± 0,367 g, SGR od 5,030 ± 0,171% i
FCE od 86,63 ± 1,300% u P. monodon koji su hranjeni s D4
(4% dodatak probiotika). Imunološki parametri određeni na
kraju eksperimenta pokazali su varijaciju među ishranama.
Ukupni broj hemocita (273,66 ± 3,09 x 105 stanica / ml),
aktivnost fenoloksidaze (0,132 ± 0,007 OD) i aktivnost
respiratornog sustava (0,291 ± 0,020 OD) je bila visoka kod
P. monodon koji su hranjeni s D4. Slični efekti zabilježeni
su za koncentraciju proteina u plazmi (68,00 ± 1,41 mg /
ml), aktivnost lizozima (0,483 ± 0,014 U / ml) i baktericidnu
aktivnost (81,0 ± 1,33%) kod rakova hranjenih s D4. Zaključci ovog istraživanja su da je probiotički produkt u koncentraciji
od 4% učinkovit za povećanje rasta i imunološke učinke
kod rakova P. monodon.The capability of a low-cost probiotic product was evaluated in Penaeus
monodon reared under an outdoor culture system for a period of 90 days.
The low-cost probiotic product was formulated by mixing the broth culture
of Bacillus cereus along with commercial chalk powder in 1:1 ratio. The
prepared probiotic product as a powdered supplement was incorporated
along with the shrimp diet at various concentrations (1 to 5%). The viability
of B. cereus in the experimental diet was tested once in 30 days up to 90
days during the experimentation. After 90 days of feeding experiments, the
growth performance of shrimp was determined with a maximum production
of 11.98 ± 0.367 g, SGR of 5.030 ± 0.171% and FCE of 86.63 ± 1.300% in
P. monodon fed D4 diet (4% probiotic supplement). The immunological
parameters determined at the end of the culture experiment showed
variation among diets. The total haemocyte count (273.66 ± 3.09 x 105
cells/ml), phenoloxidase activity (0.132 ± 0.007 OD) and respiratory burst
activity (0.291 ± 0.020 O.D) were high in P. monodon fed D4 diet. Similar
effects were observed for plasma protein concentration (68.00 ± 1.41 mg/
ml), lysozyme activity (0.483 ± 0.014 U/ml) and bactericidal activity (81.0
± 1.33%) in shrimp fed D4 diet. The results of the present investigation
conclude that the probiotic product at a concentration of 4% was effective
in elevating the growth and immune performances in shrimp P. monodon