Experimental Study, Characterization and Application of Starch-Graft-Acrylamide Gel for Plugging

During underbalance drilling, completion and workover wells, plugging channeling, blocking preformation and plugging formation water are inevitable problems. Gel is one of the most effective and convenient method to solve the problem. In this study, modified starch gel is synthesized, investigated experimentally and improved for efficient oil and gas field applications. The gel slurry is composed of starch (3.6 wt.%), initiator (0.02 wt.%), acrylamide (14.4 wt.%), cross-linking agent (4.7 wt.%), all of the components are mixed together with water at pH 10 – 11 which viscosity is as low as 35 – 82 mPa.s and desired to form gel. Here the effects of the components, reaction temperature and pH on gelation time and gel viscosity are systematically investigated, and the results showed that the gelation can be controlled in a wide range 30 – 120 min efficiently by pH and initiator. Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM) are employed to study the molecular structure and microstructure of the gel, respectively. A compact three-dimensional network structure was formed in the gel, which contribute to a good adhesion. The gel has been successfully used in shale gas field which provides a reference for sealing other similar high formation pressure under unbalanced workover treatment. DOI: http://dx.doi.org/10.5755/j01.ms.24.4.18565</p

H&amp;TECH

ABSTRACT To prevent a ball becoming stuck in coiled tubing, it is critical to determine the flow velocity of fluid in a hydraulic drive ball-off. The computational fluid dynamics method and standard k-ε turbulence model were applied to simulate the flow within the tubing of a section of coiling block. By analyzing the flow around the ball, the force on the ball exerted by fluid was computed, and the movement trend of the ball at different locations obtained by steady simulation. With the dynamic grid technique, the movement of the ball was identified through transient simulation. The research showed that: the fluid force through static simulation is too large to be used for judging the ball&apos;s passing capacity in a section of coiling block. The ball moved around the coiling block with a minimum value of θ = 90°, revealing that the ball could easily reach the highest point if the minimal angular velocity of ball was greater than zero. Moreover, the conclusion that the ball could pass through the whole section of the coiling block if only it passed the first circle of the coil could be drawn. Furthermore, the critical flow velocity for different sized ball to pass through the coiled tubing increased monotonically with the increase in the ball diameter, and exhibited an approximate quadratic relation. When the curvature radius of the coiling block was 1.268 m and the diameter of the solid steel ball was in the range of (30 mm, 50 mm), the critical flow velocity was (2.30 m/s, 2.415 m/s). This study provides a useful basis for experiment design

Structure Size Optimization and Internal Flow Field Analysis of a New Jet Pump Based on the Taguchi Method and Numerical Simulation

Interlayer contradiction (high-pressure oil that prevents low-pressure oil from being extracted) has always been the main factor affecting the oil-recovery efficiency of the many oil-bearing series in shale oil wells in Eastern Shandong, China. If steps to deal with interlayer contradiction are not taken, Shengli Oilfield’s oil-recovery efficiency will be significantly reduced after a certain period of exploitation. Furthermore, as the drilling depth increases, the formation-fluid supply capacity of Shengli Oilfield becomes worse and further increases the difficulty of oil recovery as well as production costs. In order to improve the oil-recovery efficiency of shale oil wells in Eastern Shandong and realize cost reductions and efficiency increases, we designed a new jet pump in this study. The pump can be used for oil recovery according to the principle of Venturi jet propulsion, as the required power fluid is not a high-pressure fluid injected from the ground, but rather high-pressure oil that is present in the formation. Through the analysis of the overall structure of the new jet pump, it was found that the pump could not only transform the existing interlayer contradiction (co-mining of high and low oil layers by utilizing interlayer contradiction), but also had the characteristics of a simple structure and low production costs. Since the structural dimensions of the jet pump and the physical characteristic parameters of the fluid have significant impacts on pump efficiency, we first analyzed the internal flow field of the jet pump by using numerical simulations and found that the throat–nozzle distance, area ratio, throat length–diameter ratio, diffuser angle, and flow ratio had the most significant impacts on pump efficiency. After obtaining the specific numerical range of the abovementioned structural parameters when the pump efficiency was as its maximum, an orthogonal array designed according to the Taguchi method was used to conduct experiments. According to a range analysis and an analysis of variance, at an unchanged flow ratio (0.3156), the new jet pump achieved the highest efficiency (31.26%) when the throat–nozzle distance was 2.62 mm, the throat length was 46 mm, the throat diameter was 6.8 mm, and the diffuser angle was 7.5°. In comparing its efficiency with that before optimization, we noticed that the efficiency was significantly improved by about 10%. These research results not only offer a new idea for the existing oil-recovery mode, but also introduce a new method for optimizing the structure of jet pumps

α-Glucosidase Inhibitors from Two Mangrove-Derived Actinomycetes

α-Glucosidase (AGS) inhibitors have been regarded as an ideal target for the management of type 2 diabetes mellitus (T2DM) since they can maintain an acceptable blood glucose level by delaying the digestion of carbohydrates and diminishing the absorption of monosaccharides. In the process of our endeavor in mining AGS inhibitors from natural sources, the culture broth of two mangrove-derived actinomycetes Streptomyces sp. WHUA03267 and Streptomyces sp. WHUA03072 exhibited an apparent inhibitory activity against AGS. A subsequent chemical investigation into the two extracts furnished 28 secondary metabolites that were identified by spectroscopic methods as two previously undescribed linear polyketides 1–2, four benzenoid ansamycins 3–6, fourteen cyclodipeptides 7–18, one prenylated indole derivative 19, two fusicoccane-type diterpenoids 20–21, two hydroxamate siderophore 22–23, and five others 24–28. Among all of the isolates, 11 and 24 were obtained from actinomycetes for the first time, while 20–21 had never been reported to occur in a marine-derived microorganism previously. In the in vitro AGS inhibitory assay, compounds 3, 8, 9, 11, 14, 16, and 17 exhibited potent to moderate activity with IC50 values ranging from 35.76 ± 0.40 to 164.5 ± 15.5 μM, as compared with acarbose (IC50 = 422.3 ± 8.4 μM). The AGS inhibitory activity of 3, 9, 14, 16, and 17 was reported for the first time. In particular, autolytimycin (3) represented the first ansamycin derivative reported to possess the AGS inhibitory activity. Kinetics analysis and molecular docking were performed to determine the inhibition types and binding modes of these inhibitors, respectively. In the MTT assay, 3, 8, 9, 11, 14, 16, and 17 exhibited no apparent cytotoxicity to the human normal hepatocyte (LO2) cells, suggesting satisfactory safety of these AGS inhibitors

&alpha;-Glucosidase and Bacterial &beta;-Glucuronidase Inhibitors from the Stems of Schisandra sphaerandra Staph

&alpha;-Glucosidase (AGS) is a therapeutic target for Type 2 diabetes mellitus (T2DM) that tends to complicate with other diseases. Some medications for the treatment of T2DM complications have the risk of inducing severe adverse reactions such as diarrhea via the metabolism of intestinal bacterial &beta;-glucuronidase (BGUS). The development of new AGS and/or BGUS inhibitors may improve the therapeutic effects of T2DM and its complications. The present work focused on the isolation and characterization of AGS and/or BGUS inhibitors from the medicinal plant Schisandra sphaerandra. A total of eight compounds were isolated and identified. Sphaerandralide A (1) was obtained as a previously undescribed triterpenoid, which may have chemotaxonomy significance in the authentication of the genus Schisandra and Kadsura. 2&prime;-acetyl-4&prime;,4-dimethoxybiphenyl-2-carbaldehyde (8) was obtained from a plant source for the first time, while compounds 2&ndash;7 were isolated from S. sphaerandra for the first time. In the in vitro assay, compounds 1&ndash;5 showed potent to moderate activity against AGS. Interestingly, compound 3 also exhibited significant BGUS inhibitory activity, demonstrating the potential of being developed as a bifunctional inhibitor that may find application in the therapy of T2DM and/or the diarrhea induced by medications for the treatment of T2DM complications