Recombinational Repair of a Chromosomal DNA Double Strand Break: A Dissertation

Repairing a chromosomal DNA double strand break is essential for survival and maintenance of genomic integrity of a eukaryotic organism. The eukaryotic cell has therefore evolved intricate mechanisms to counteract all sorts of genomic insults in the context of chromatin structure. Modulating chromatin structure has been crucial and integral in regulating a number of conserved repair processes along with other fundamental genomic processes like replication and transcription. The work in this dissertation has focused on understanding the role of chromatin remodeling enzymes in the repair of a chromosomal DNA double strand break by homologous recombination. This has been approached by recapitulating the biochemical formation of recombination intermediates on chromatin in vitro. In this study, we have demonstrated that the mere packaging of DNA into nucleosomal structure does not present a barrier for successful capture of homologous DNA sequences, a central step of the biochemical pathway of recombinational repair. It is only the assembly of heterochromatin-like more complex nucleo-protein structure that presents additional constraints to this key step. And, this additional constraint can be overcome by the activities of ATP-dependent chromatin remodeling enzymes. These findings have great implications for our perception of the mechanism of the recombinational repair process of a chromosomal DNA double strand break within the eukaryotic genome

Unitary sources say: It is inhibition!

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/162710/2/tjp14269.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/162710/1/tjp14269_am.pd

How can power discourses be changed?

Social policy impact is partly determined by how policy is articulated and advocated, including which values are highlighted and how. We examine the influence of policy framing and reframing on outcomes, with particular reference to policies of the Delhi state government in India that target the practices of female feticide, infanticide and neglect that underlie the ‘daughter deficit’. Using Snow and Benford’s categories for understanding reframing processes, the paper outlines and applies a ‘model’ of reframing disputed issues, derived from looking at two famous campaigns – Gandhi’s 1930 Salt March in the struggle for Indian freedom from British rule and the African- American civil rights struggle of the 1950s and 60s. It argues that ‘carrot and stick’ policy measures, such as financial incentives and legal prohibitions, to counteract the ‘daughter deficit’ must be complemented by well crafted discursive interventions

Utilization of modern temporary contraceptive methods and its predictors among reproductive-aged women in India: insights from NFHS-5 (2019–21)

Evidence from various studies on modern contraceptive methods shows that the utilization varies greatly. The present study aimed to estimate the magnitude and determinants for temporary modern contraceptive utilization among reproductive-aged (15-49 years) women in India. We analysed National Family Health Survey-5 data using the “svyset” command in STATA software. Modern contraception utilization was estimated using the weighted prevalence, and its correlates were assessed by multivariable regression by reporting an adjusted prevalence ratio (aPR) with 95% confidence interval (CI). QGIS 3.2.1 software was used for spatial analysis of different temporary modern contraceptives. The mean (SD) age of 359,825 respondents was 31.6 (8.5) years with 75.1% (n = 270,311) and 49.2% (n = 177,165) of them being from rural area and having completed education up to secondary school, respectively. The overall utilization of modern temporary contraception was 66.1% [95%CI: 65.90–66.35, n = 237,953]. Multigravida (vs. nulligravida) [aPR = 2.13 (1.98–2.30)], higher education of husband (vs. not educated) [aPR = 1.20 (1.14–1.27)], urban (vs. rural) [aPR = 1.06 (1.03–1.10)], watching television less than once a week (vs. not at all) [aPR = 1.04 (1.01–1.08)], divorced (vs. married) [aPR = 0.65 (0.45–0.94)], and Scheduled Tribe (ST) (vs. unreserved) [aPR = 0.92 (0.88–0.96)] were significant independent determinants. The highest utilization of male condoms, IUCDs, pills and injections were in Himachal Pradesh (86%), Nagaland (64%), Tripura (85%), and Ladakh (20%), respectively. Out of every ten reproductive-aged (15–49 years) women in India, six are using temporary modern contraceptive methods. More intervention strategies should be planned, considering factors like gravida, education, residence, health promotion and caste to attain replacement fertility level

How can power discourses be changed? - Contrasting the ‘daughter deficit’ policy of the Delhi government with Gandhi and King’s transformational reframing

Social policy impact is partly determined by how policy is articulated and advocated, including which values are highlighted and how. In this paper, we examine the influence of policy framing and reframing on outcomes, with particular reference to the policies of the Delhi state government in India that target the practices of female feticide, infanticide and neglect that underlie the ‘daughter deficit’. Using Snow and Benford’s categories for understanding reframing processes, the paper outlines and applies a ‘model’ of reframing disputed issues derived from looking at two famous campaigns – Gandhi’s 1930 Salt March in the struggle for Indian freedom from British rule and the African-American civil rights struggle of the 1950s and 1960s. It argues that ‘carrot and stick’ policy measures, such as financial incentives and legal prohibitions, to counteract the ‘daughter deficit’ must be complemented by well crafted discursive interventions

Unilateral high division of the Sciatic Nerve with divided Piriformis

Abstract While doing the routine dissection for the undergraduate students in the department of Anatomy, Nil Ratan Sircar Medical College, Kolkata, few variations were found in the gluteal region of a 70 years old male cadaver, in the year 2013. On the right side the sciatic nerve (SN) pierced the piriformis muscle dividing it into superior and inferior slips and then, after a short distance, divided into tibial and common peroneal nerves in the gluteal region. On the left side, sciatic nerve divided into two terminal branches (common peroneal and tibial nerves) in the lower part of the back of the thigh near the apex of the popliteal fossa as usual. This high division of the sciatic nerve may result in nerve injury during deep intramuscular injections in gluteal region, piriformis syndrome due to compression of the nerve, failed SN block in anesthesia and surgical complications