How to Identify Rare and Endangered Ferns and Fern Allies

Identification of rare and endangered plant species is the first requirement for any conservation programme. The IUCN guideline is the only available method to identify the rare and endangered species and it requires vast data on the wild population of the target species. None of the biological characters, which are playing main role in the survival and distribution of several species, is used in IUCN guideline. In the meantime there are several difficulties in following IUCN guideline, particularly the non availability of complete field data. Moreover, the same guideline can not be used for all the groups of species in equal importance. The vascular cryptogams, pteridophytes, are also an important component of any mountainous flora and they have also to be conserved in nature. As they are the primitive vascular plants on the earth, they are getting depleted in the flora due to various reasons and it is the right time to identify the rare and endangered pteridophytes to conserve them. By considering various difficulties of IUCN method for the identification rare and endangered pteridophytes, a very simple method has been adopted by using just four criteria and this method can be applied to Pteridophytes from any region of the world

Inhibition and kinetic studies of cellulose - and hemicellulose - degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds

AIM: Ganoderma sp, the causal pathogen of the basal stem rot (BSR) disease of oil palm, secretes extracellular hydrolytic enzymes. These play an important role in the pathogenesis of BSR by nourishing the pathogen through the digestion of cellulose and hemicellulose of the host tissue. Active suppression of hydrolytic enzymes secreted by Ganoderma boninense by various naturally occurring phenolic compounds and estimation of their efficacy on pathogen suppression is focused in this study. METHODS AND RESULTS: Ten naturally occurring phenolic compounds were assessed for their inhibitory effect on the hydrolytic enzymes of G. boninense. The enzyme kinetics (Vmax and Km ) and the stability of the hydrolytic enzymes were also characterized. The selected compounds had shown inhibitory effect at various concentrations. Two types of inhibitions namely uncompetitive and noncompetitive were observed in the presence of phenolic compounds. Among all the phenolic compounds tested, benzoic acid was the most effective compound suppressive to the growth and production of hydrolytic enzymes secreted by G. boninense. The phenolic compounds as inhibitory agents can be a better replacement for the metal ions which are known as conventional inhibitors till date. The three hydrolytic enzymes were stable in a wide range of pH and temperature. CONCLUSION: These findings highlight the efficacy of the applications of phenolic compounds to control Ganoderma. SIGNIFICANCE AND IMPACT OF THE STUDY: The study has proved a replacement for chemical controls of G. boninense with naturally occurring phenolic compounds

Glimpses of Tribal Botanical Knowledge of Tirunelveli Hills, Western Ghats, India

In the present paper, 46 plant species of angiosperms belonging to 19 genera of Euphorbiaceae that occur naturally in the Tirunelveli Hills of western Ghats, India, were chosen for study. It was found that the uses of Euphorbiaceous plants by the inhabitants of this region cover a number of broad categories including food, various kinds of poisons, medicines, sundry types of oils, waxes, rubbers, varnishes, compounds for paints and other industrial products

Overexpression Analysis of emv2 gene coding for Late Embryogenesis Abundant Protein from Vigna radiata (Wilczek)

Late embryogenesis abundant (LEA) proteins are speculated to protect against water stress deficit in plants. An over expression system for mungbean late embryogenesis abundant protein, emv2 was constructed in a pET29a vector, designated pET-emv2 which is responsible for higher expression under the transcriptional/translational control of T7/lac promoter incorporated in the Escherichia coli BL21 (DE3).Induction protocol was optimized for pET recombinants harboring the target gene. Overexpressed EMV2 protein was purified to homogeneity and the protein profile monitored by SDS-PAGE

Functional and radiological analysis of posterior lumbar interbody fusion in spondylolisthesis

Background:Spondylolisthesis is present in 5% of the adult population with clinical evidence of low back pain. These patients are treated initially by conservative measures, failing of which surgical intervention is mandatory. Majority of patients with varying degree of slip and disability ultimately require surgical intervention. In this study we are trying to analyse the functional outcome following posterior lumbar interbody fusion in spondylolisthesis.Methods: Posterior lumbar interbody fusion using pedicle screw and rods with cage was performed on 25 patients. 6 months follow-up was completed in 25 patients who were then reviewed at regular intervals. Out of the 25 patients, 17(68%) were females and 8(32%) were males. The mean age of the patients was 40.64 years. Out of 25 patients, 14 patients had listhesis at L4 – L5 level and another 11 at L5 – S1 level. 21(84%) were Isthmic variant and 4 (16%) were Degenerative spondylolisthesis.Results:The mean follow up period in this study of 25 patients is 19 months. Out of 25 patients, there was mean improvement of 18.96 in the Oswestry scoring index. The Visual analogue scale score showed a mean improvement of 6.48. Radiologically, the percentage of slip was decreased by a mean of 8.40%. One patient had a cage extrusion with no neurological deficit.Conclusions:The pedicle screw with rod and cage system is easy to use and provides the anatomic restoration of the isthmus in isthmic spondylolisthesis or restoring the stability after laminectomy/discectomy in degenerative spondylolisthesis. From our study, we strongly believe that this technique is very useful in low grade degenerative and isthmic spondylolisthesis

Inhibition and kinetic studies of lignin degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds

Aim Lignolytic (lignin degrading) enzyme, from oil palm pathogen Ganoderma boninense Pat. (Syn G. orbiforme (Ryvarden)), is involved in the detoxification and the degradation of lignin in the oil palm and is the rate‐limiting step in the infection process of this fungus. Active inhibition of lignin‐degrading enzymes secreted by G. boninense by various naturally occurring phenolic compounds and estimation of efficiency on pathogen suppression was aimed at. Methods and Results In our work, 10 naturally occurring phenolic compounds were evaluated for their inhibitory potential towards the lignolytic enzymes of G. boninense. Additionally, the lignin‐degrading enzymes were characterized. Most of the peholic compounds exhibited an uncompetitive inhibition towards the lignin‐degrading enzymes. Benzoic acid was the superior inhibitor to the production of lignin‐degrading enzymes, when compared between the 10 phenolic compounds. The inhibitory potential of the phenolic compounds towards the lignin‐degrading enzymes are higher than that of the conventional metal ion inhibitor. The lignin‐degrading enzymes were stable in a wide range of pH but were sensitive to higher temperature. Conclusion The study demonstrated the inhibitor potential of 10 naturally occurring phenolic compounds towards the lignin‐degrading enzymes of G. boninense with different efficacies. Significance and Impact of the Study The study has shed a light towards a new management strategy to control basal stem rot disease in oil palm. It serves as a replacement for the existing chemical control

Comparing the influence of natural farming on cotton (Gossypium hirsutum) yield and economics with integrated and organic farming

Cotton, a vital global cash crop, influences the economy and sustainability. Natural farming is a cost-effective, eco-friendly method. This study examines Natural Farming's effects on cotton yield and the economics of cotton compared to Integrated and organic farming. Field experiments were conducted during 2021-22 and 2022-23 at Tamil Nadu Agricultural College, Coimbatore. The experiment consisted of eight treatments, laid out in Randomized Block Design and replicated thrice. The treatments consist of control (T1), various Indian Natural farming practices including Beejamirit as a seed treatment, Ghanajeevamirit as basal, Jeevamirit with irrigation water, crop residue mulch, intercroping with pulses (T2 to T5), Organic farming practices (T6), Integrated crop management (ICM) practices with organic pest control (T7) and ICM with chemical pest (T8). The results indicated a significant increase in yield components: sympodial branches plant-1 (12.85 and 13.40), number of squares (29.28 and 31.28), bolls plant-1 (10.74 and 11.32), and boll weight (4.30 and 4.38 g) under Integrated Crop Management practices in 2021-22 and 2022-23, respectively. However, organic farming practices, followed by natural farming practices, were at par with integrated crop management. Integrated crop management practices resulted in significantly higher seed cotton yields (2178 and 2232 kg/ha in 2021-22 and 2022-23, respectively) than organic and natural farming practices with a higher cost of cultivation and a gross return. Complete natural farming outperformed organic and integrated farming in cotton, yielding higher net returns and B: C ratios. This approach holds promise for enhancing yield, economic viability and environmental sustainability in cotton cultivation

Spectral Signature of Wind Generation From The Post-Shock Region in GRS1915+105 Accretion Disk

Accretion and outflows are common in systems which include black holes. Especially important is the case of the well known micro-quasar GRS1915+105 in our own galaxy, where super-luminal outflows are detected. We present a few observation which are suggestive of an outflow which is generated very close to the black hole, within a few tens of Schwarzschild radii. In the presence of mass loss (e.g., an outflowing wind), the electron density of matter within the centrifugal pressure supported region (which generates hard X-rays) goes down and it is easier to cool these electrons by soft photons coming from the Keplerian disk. If, on the other hand, the post-shock region gains mass from outside, the spectra would be harder. These properties of spectral `softening' of the low state and `hardening' of the high state have been detected in several days of RXTE data of GRS1915+105 which we present here.Comment: 10 latex pages with four figures (Submitted Astronomy and Astrophysics, May 2000