PLANT LEAF ASSISTED SYNTHESIS AND APPLICATION EVALUATION OF SILVER NANOPARTICLES

Abstract- Use of the plant material for the bioreduction of the metal for the nanoparticles synthesis is currently the choice of methods in bionanotechnology. In the present study, silver nanoparticles (AgNPs) were rapidly synthesized usingan aqueous extract of Ocimum sanctum (Tulsi) and Partheniumhysterophorous(Congress grass). The synthesis process was standardizedbased on the trials. The process of AgNPs formation usingwith Tulsi aqueous extract was carried out with 0.1 mMAgNO3solution and referred as a T1 sample,whereas 2.0 mMAgNO3was used with the Congress grass aqueous extract as a C4 sample.These AgNPs were characterized by means of UV-Vis spectroscopy, scanning electron microscopy(SEM), electron diffraction spectroscopy (EDX) and Dynamic light scattering (DLS).AgNPsformation was confirmed by Surface Plasmon Resonance as determined by UV–Visible spectra analysis in the range of 250 to 750 nm. Maximum absorbance (λ max) peak was reported between 406 to 446 nm in T1 and C4 samplesfollowed by SEM characterization showed a uniform distribution of nanoparticles, with an average size of 68.74 nm and 108.6 nm in T1 and C4 samples respectively. Average size measurement was confirmed by DLS and the presence of silver was confirmed by EDX.Application including mice spleenocytes viability, bactericidal effects, Synergistic effect with Antibiotic and pesticides adsorption suggest the wide scope AgNPs in various fields