Rodent β-amyloid (Aβ) staining following cortical photothrombosis.

<p>Digital photomicrographs showed atypical, Aβ deposits in the thalamus ipsilateral to the cortical lesion (<b>A–D</b>). The inserts <b>A1–D1</b> at higher magnification are taken from the same brain sections. The genotype had a significant effect on rodent Aβ load (two-way ANOVA, <i>P</i><0.05) being more pronounced in non-transgenic mice (<b>E</b>). Values are presented as mean±s.e.m. Scale bar: 500 µm (<b>A–D</b>), 20 µm (<b>A1–D1</b>).</p

Rose Bengal induced cortical photothrombosis.

<p>(<b>A</b>) The cold light was positioned to illuminate the skull 2.4 mm right from Bregma. (<b>B</b>) Nissl-stained sections show a typical photothrombotic lesion in the right sensorimotor cortex (arrows). (<b>C</b>) The genotype had a significant effect on lesion size (two-way ANOVA, <i>P</i><0.05) with smaller lesions in transgenic mice. Values are presented as mean±s.e.m. Scale bar: 1 mm (<b>B</b>).</p

Confirmation of the transgenic Aβ phenotype.

<p>Human specific W0-2 staining against Aβ showed a relative high staining intensity in transgenic mice due to intracellular Aβ typical to young transgenic animals (A). A few plaques were observed (arrows). Only some non-specific staining was observed in non-transgenic mice due to degenerative processes (<b>B</b>). Scale bar: 400 µm.</p

Calcium staining (Alizarin Red) following cortical photothrombosis.

<p>Digital photomicrographs showed atypical, calcium deposits in the thalamus ipsilateral to the cortical lesion (<b>A–D</b>). The inserts <b>A1–D1</b> at higher magnification are taken from the same brain sections. Treatment had a significant effect on calcium accumulation (two-way ANOVA, <i>P</i><0.001) (<b>E</b>). Values are presented as mean±s.e.m. Scale bar: 500 µm (<b>A–D</b>), 20 µm (<b>A1–D1</b>).</p

Cellular localization of rodent Aβ deposits in the thalamus following cortical photothrombosis.

<p>Double immunofluorescence staining for rodent Aβ and NeuN <b>(A)</b> or rodent Aβ and GFAP <b>(B)</b> did not show co-localization in AD transgenic mice (arrows).</p

Uptake of MLNPs in the rat body.

<p>Two coronal MRI slices through the rat body are shown. Following MLNP injection, a significant and long lasting signal increase (arrows) was mostly observed in the liver L (row <b>A</b>) and in the spleen S (row <b>B</b>).</p

T₁ histogram (dotted line) of a rat brain.

<p>Triple Gaussian fitting allowed for separation of white matter (circles), gray matter (diamonds) and cerebrospinal fluid (squares).</p

SEM images (Tescan MIRA XMU with YAG BSE-detector and SE-detector) of HSA nanoparticles labeled with magnetite (8 nm).

<p>The light spots show the incorporated magnetite. Upper image: 20,000-fold magnification with 5.78 μm field of view, lower image: 60,000-fold magnification with 1.93 μm field of view.</p

Relaxivity of the different magnetically labeled nanoparticle formulations.

<p>Relaxivity of the different magnetically labeled nanoparticle formulations.</p

Prussian Blue staining close to the injection site of the rat shown in Figure 8 in a vehicle control rat.

<p>(<b>A</b>) Slice fluorescence levels are generally low in the cryo slices and in most cases restricted to vasculature as shown here for some ependymal cells of the chorioid plexus. (<b>B</b>) shows the Prussian Blue staining, whereas in healthy rats iron accumulations (from micro bleedings) in the brain are typically absent (<b>C</b>) is a maximum difference projection of (<b>A</b>) and (<b>B</b>). (<b>D</b>) The slice fluorescence vanishes during Prussian Blue staining and brightfield imaging. Abbreviations: F  =  fluorescence; LV  =  lateral ventricle; CC  =  corpus callosum. Magnification  =  20x.</p