2 research outputs found
Alleviative effect of myricetin on ochratoxin A-induced oxidative stress in rat renal cortex: histological and biochemical study
Ochratoxins (OTA) are secondary
metabolites of Aspergillus and Penicillium. The
detoxification of OTA has been of major interest due to
its widespread threat to human health. We aimed to
investigate the possible alleviative effect of myricetin
(MYR) against OTA-induced damage in renal cortex of
rats. Thirty adult male albino rats were randomized into
five equal groups: control (untreated), vehicle control
(0.5 ml corn oil/day including dimethylsulfoxide
[DMSO]), MYR (100 mg MYR/kg b.w./day in distilled
water), OTA (0.5 mg OTA/kg b.w./day; dissolved in 10%
DMSO and then corn oil) and OTA + MYR group
(received OTA and MYR at similar doses). All
treatments were given by oral gavage for 2 weeks. At the
end of the experiment, renal cortices were processed for
light and electron microscope examinations.
Immunohistochemical staining for localization of
proliferating cell nuclear antigen (PCNA), p53 and
transforming growth factor beta 1 (TGF-β1) was carried
out. Biochemical analysis of tissue glutathione
peroxidase (GPX), catalase (CAT) and superoxide
dismutase (SOD) were determined to evaluate oxidative
stress. OTA administration induced deleterious renal
injury evidenced by the structural and ultra-structural
changes. Immunohistochemical expression of p53,
PCNA and TGF-β1 were significantly up regulated
compared with control. Alterations in antioxidant
parameters supported that oxidative stress was one of the
mechanisms involved in OTA toxicity. On the contrary,
co-administration of MRY partially ameliorated OTAinduced renal injury. We suggest the potential
effectiveness of MYR to counteract OTA-induced toxic
oxidative stress on the renal cortex