СКРИНИНГ НОВЫХ ХИМИОТЕРАПЕВТИЧЕСКИХ СРЕДСТВ ДЛЯ БОРЬБЫ С БОЛЕЗНЯМИ РЫБ, ВЫЗЫВАЕМЫМИ ПАРАЗИТИЧЕСКИМИ ДИПЛОМОНАДИДАМИ (DIPLOMONADIDA WENYON, 1926)

Objective of research. The purpose of the study – screening of new chemotherapeutic agents for the treatment of hexamita ornamental fish. Materials and methods. The test drugs were performed on juveniles of the discus Symphysodon discus a body mass of 15 g, spontaneously infested hexamitidae (Diplomonadida). 35 discus of the infected individuals were divided into 7 groups of 5 copies of the Fish 1, 2, 3, 4, 5, and 6th groups were appointed in the composition of granulated feed, respectively pyrimethamine at a dose of 50 mg/kg albendazole – 50, fenbendazole – 50, mebendazole – 10, magnesium sulfate – 500 and metronidazole in a dose of 50 mg/kg for 5 days. Fish 7 the first group was control and received feed without the drug. The effectiveness was considered after 5 days after the last Dachi medicated feed. Fish were dissected, the intestines were isolated, which were released from the food pulp, homogenized, and were studied by optical microscopy at a magnification of 180 times. Results and discussion. The number of examined in treated fish compared with the control decreased after the application of metronidazole by 95.5 %, mebendazole – 77.8, magnesium sulfate, which is 74.5, fenbendazole – by 56.6, albendazole – 50.5 and pyrimethamine – 24.4 %. Advanced for further testing are mebendazole, magnesium sulphate, fenbendazole and albendazole. The most effective was a basic drug – metronidazole. During the test degradation of fish were noted. Food activity was moderate at the level of 2% of ichthyomass. In fish treated with metronidazole, mebendazole and magnesium sulfate, have improved the condition.Цель исследования – скрининг новых химиотерапевтических средств для лечения гексамитоза декоративных рыб. Материалы и методы. Испытание препаратов проводили на молоди дискуса Symphysodon discus массой тела 15 г, спонтанно инвазированных гексамитидами (Diplomonadida). 35 зараженных особей дискуса разделили на 7 групп по 5 экз. Рыбам 1, 2, 3, 4, 5 и 6-й групп назначали в составе гранулированного корма соответственно пириметамин в дозе 50 мг/кг, альбендазол – 50, фенбендазол – 50, мебендазол – 10, сульфат магния – 500 и метронидазол – в дозе 50 мг/кг в течение 5 сут. Рыба 7-й группы была контролем и получала корм без препарата. Эффективность препаратов учитывали через 5 сут после последней дачи лечебного корма. Рыбу вскрывали, выделяли кишечник, который освобождали от кормовых масс, гомогенизировали и микроскопировали при увеличении в 180 раз. Результаты и обсуждение. Число гексамитид у леченой рыбы по сравнению с контролем снизилось после применения метронидазола на 95,5 %, мебендазола – на 77,8, сульфата магния – на 74,5, фенбендазола – на 56,6, альбендазола – на 50,5 и пириметамина – на 24,4 %. Перспективными для дальнейших испытаний являются мебендазол, сульфат магния, фенбендазол и альбендазол. Наиболее эффективным оказался базовый препарат – метронидазол. В период испытания ухудшения состояния рыб не отмечали. Пищевая активность была умеренной, на уровне 2 % от ихтиомассы. У рыб, леченных метронидазолом, мебендазолом и сульфатом магния, улучшилось состояние здоровья

Application of impedance spectroscopy method for analysis of benzanol fuels

The authors have developed a method for express control of three component «gasoline-alcohol-water» fuel mixtures based on the spectral impedance investigation of benzanol mixture in the frequency range of 500 Hz — 10 kHz. A correlation dependence between the dielectric constant and the specific resistance of the fuel mixture on content of ethanol and water in the mixture has been found. On the basis of this dependence a calibration nomogram to quantify the gasoline and water-alcohol components content in the test benzanol fuel in the actual range of concentrations has been formed. The nomogram allows determining the water-alcohol and gasoline parts in the analyzed fuel with an error of no more than 1% vol., while the strength of water-alcohol solution is determined with an error of no more than 0.8% vol. The obtained nomogram can also give information about critical water content in the benzanol fuel to prevent its eventual phase separation. It is shown that the initial component composition of different gasoline brands has no significant effect on the electrical characteristics of the studied benzanol fuels, which makes the evaluation of alcohol and water content in the fuel sufficiently accurate. for practical applications