2 research outputs found
MIC susceptility testing of nosocomial infections at PICU in Children's Medical Center, Iran
Background: The aim of this study was to determine the minimal
inhibitory concentration (MIC) of microorganisms causing nosocomial
infections in the pediatric intensive care unit (PICU) of the
Children's Medical Center in Tehran. Methods: All patients with
nosocomial infections in the PICU were enrolled in the study. Causative
microoraganisms were coagulase positive, and coagulase negative
Staphylococci and Pseudomonas aeruginosa . MIC of many antibiotics
was determined by microbroth dilution according to NCCLS. Findings:
Within a period of 18 months, thirty patients developed nosocomial
infection including 17 cases with P.aeruginosa and 9 individuals with
Staph aureus infection. The remaining 4 patients were involved with
coagulase negative Staphylococci. The most common sources were
respiratory tract, blood stream, wound and soft tissue.
Multi-antimicrobial resistance (resistance to Amikacin, Ceftazidim,
Imipenem and Ciprofloxacin) was common among P. aeruginosa species All
strains of Staph aureus were resistant to Methicillin (MRSA). These
microorganisms were also resistant to clindamycin and ciprofloxacin in
88% of cases.Conclusion: Resistance to antimicrobial agents was high in
our study, therefore routine MIC examination is necessary in PICU
Study of antibiotics resistance in pediatric acute bacterial meningitis with E-Test method
Background: Appropriate treatment of bacterial meningitis especially in
children is a important problem due to multiple drug resistance. The
determination MIC of conventional antibiotics for bacterial meningitis
with quantitative E. test is exactly practical and essential. We
studied MIC of conventional antibiotics in pediatric acute bacterial
meningitis older than two months, center children hospital,
1382-1384.Methods: In this prospective and cross sectional process
research we measured MIC of antibiotics in 30 positive bacterial
culture in CSF or blood with quantitative E. test and compared with
qualitative disk diffusion test. Findings: Antibiotic resistance of 10
Haemophilus influenzae type b patient was : ampicillin resistance in
90%, co – Amoxiclave R. in 10%, chlorampheicol R. in 40%, third
gerenation cephlosporins R.( ceftriaxone and cefotaxime) in 0% and
cotrimoxazole R. in 100% and antibiotic resistance of 20 streptococcous
pneumoniae patient was :penicillin R. in 35% , chloramphenical R. in
10%, third generation cephalosporins R. in 5%, rifampin R. in 10% and
cotrimoxazole R. in 60% .In comparison of two methods E-Test and Disk
Diffusion we found insignificant difference. Conclusions: Antibiotic
Resistance of our study is compatible with other studies .Therefore it
seems that composition of ampicillin and chloramphenicol as empiric
therapy for bacterial meningitis for many years ago, aren't appropriate
today and third generation cephalosporins alone or with vancomycin is a
suitable therapy