14 research outputs found
Characterisation of morbilliviruses isolated from Lake Baikal seals (Phoca sibirica).
Sequence analysis of the haemagglutinin protein (H) gene of the morbillivirus (PDV-2) isolated from a Siberian seal (Phoca sibirica) during the 1987/1988 epizootic in Lake Baikal revealed that it was most closely related to two recent isolates of canine distemper virus (CDV) from Germany and different from CDV vaccines currently in use in that region. The virus continued to circulate in seals in Lake Baikal after the 1987/1988 epizootic since sera collected from culled seals in the spring of 1992 were positive in morbillivirus ELISA tests, reacting most strongly with the CDV antigen
Quantum Vacuum Experiments Using High Intensity Lasers
The quantum vacuum constitutes a fascinating medium of study, in particular
since near-future laser facilities will be able to probe the nonlinear nature
of this vacuum. There has been a large number of proposed tests of the
low-energy, high intensity regime of quantum electrodynamics (QED) where the
nonlinear aspects of the electromagnetic vacuum comes into play, and we will
here give a short description of some of these. Such studies can shed light,
not only on the validity of QED, but also on certain aspects of nonperturbative
effects, and thus also give insights for quantum field theories in general.Comment: 9 pages, 8 figur
Interphase ribosomal RNA cistron silver staining in refractory anaemias with and without excess blasts.
Interphase ribosomal RNA cistron staining in thyroid epithelial cells in Grave's disease, Hashimoto's thyroiditis and benign and malignant tumours of the thyroid gland
Canine distemper virus in Lake Baikal seals (Phoca sibirica).
The virus epizootic which resulted in significant mortality in Siberian seals (Phoca sibirica) in Lake Baikal during 1987/88 was caused by canine distemper virus. Sequence analysis of the virus glycoprotein genes revealed that it was most closely related to recent European field isolates of canine distemper virus. This paper presents evidence that the same virus continued to circulate in seals in Lake Baikal after the initial epizootic. Three out of 45 brain tissue samples collected from seals culled in the spring of 1992 were positive for canine distemper virus-specific nucleic acid by the reverse transcription/polymerase chain reaction and the sequences were closely related to that of the original virus isolated in 1988
Evaluation of argyrophilic nucleolar organiser regions (AgNORs) in multiple myeloma
Aim—To investigate the prognostic value of argyrophylic nucleolar organiser regions (AgNORs) in multiple myeloma. Methods—Bone marrow aspirates from 55 newly diagnosed patients with multiple myeloma were stained with the one step AgNO(3) technique. The mean number of AgNORs in each plasma cell nucleus (AgNOR count) was tested for a possible correlation with other clinical and laboratory variables at presentation (clinical stage, substage, heavy and light chain isotype, haemoglobin concentration, platelet count, marrow infiltration rate, degree of skeletal lesions, M protein concentration, plasma cell morphology, and serum concentrations of calcium, albumin, lactate dehydrogenase, C reactive protein, and ß(2) microglobulin) and with outcome (response to first line treatment, first remission duration, and overall survival). Results—A significant association between mean (SD) AgNOR count was found only for clinical stage (stage I, 3.09 (1.19); stage II, 3.80 (1.53); stage III, 5.28 (1.79); p < 0.005) and, from all stage determinants, only for M protein concentration (high, 5.92 (1.80); low, 4.01 (1.92); p < 0.001). There was a linear relation between AgNOR count and serum M protein concentration for patients with both IgG (r = 0.450; p < 0.01) and IgA (r = 0.768; p < 0.002) producing multiple myeloma. Conclusions—Unlike previous investigations, no clear prognostic value for the AgNOR count was found in multiple myeloma. Instead, the results indicate that the AgNOR count might be an index for M protein synthesis rate. This is consistent with other findings in tissues with low proliferative potential and high protein synthetic activity, and calls for a cautious interpretation of AgNORs in malignancies with similar features. Key Words: argyrophilic nucleolar organiser regions, multiple myeloma • M protein synthesis • prognosi