35 research outputs found

    SATHI- Animation Music Video

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    Sathi is a short animated music video inspired by the life of the creator. The story is all about an introverted boy and his love for music which helps him to express his emotions. The emotions, expressions with the magic of animation are the keywords by which the whole story has been told. The work is done in traditional animation and digital, both. It‟s mixed media work in which software used as a mediator to produce the required output. Music is also a very important part of this project because when this idea of showing my story in the form of animation came in mind is, music was the lead keyword for the work. According to music, and the feel of the story lyrics have been written. In the process of doing this project, I explored many treatments and medium to construct a good story. This movie is a try to make the life story of a writer in reach of his target audience. The story begins with the fear of an introverted boy and his secret love for music. The movie continues as to how this introverted boy gets involved in music and how music impacted his life

    A Review on Secure Access to Cloud Storage by using ABE

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    Cloud computing is going to be very famous technology in IT enterprises. For an enterprise, the data stored is huge and it is very precious. All tasks are performed through networks. Hence, it becomes very important to have the secured use of data. In cloud computing, the most important concerns of security are data security and privacy. For access control, being one of the classic research topics, many schemes have been proposed and implemented. In this paper, various schemes for encryption that consist of Attribute based encryption (ABE) and its types KP-ABE, CP-ABE is explored. Public Key Encryption acts as the basic technique for ABE where it provides one to many encryptions, here, the private key of users & the cipher-text both rely on attributes such that, when the set of the attributes of users key matches set of attributes of cipher-text with its corresponding access policy, only then decryption is possible

    Correlation between estimated glomerular filtration rate and serum electrolytes in chronic kidney disease patients

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    Background: The occurrence of chronic kidney disease (CKD) a highly prevalent condition has been escalating in recent years. Electrolytes are the key to homeostasis and furthermore, their regulation is dependent upon renal function. CKD is associated with aberrations in the metabolism of electrolytes such as calcium, magnesium, sodium, and potassium. Aims and Objectives: The aim of this study is to study the correlation between estimated glomerular filtration rate (eGFR) and serum electrolytes in CKD patients. Materials and Methods: The present study was a cross-sectional study. One hundred patients with CKD aged 18 years or above were enrolled with inclusion and exclusion criteria. Complete blood investigations, urine analysis and ultrasonographic findings for detection of CKD were done and a semi-structured pro forma was used to record clinical profile in a cross-sectional study and patients were grouped into their respective CKD stages based on their eGFR. Results: The mean value of eGFR was 40.92±21.35 mL/min/1.73 m2. The mean value of serum sodium, calcium, magnesium, and potassium was 137.1±2.16 meq/L, 9.15±0.61 mg/dL, 3.16±0.93 mg/dL, and 4.65±0.96 meq/L, respectively. In the present study, we found that the mean serum magnesium levels in Stages 1, 2, 3, 4, and 5 of CKD were 2.10±0.20, 2.10±0.28, 2.74±0.50, 3.95±0.54, and 4.66±0.43 mg/dL, respectively. Mean serum magnesium levels were the mean serum potassium levels in Stages 1, 2, 3, 4, and 5 of CKD were 4.13±0.57, 4.15±0.68, 3.86±0.41, 5.55±0.39, and 6.25±0.08 meq/L, respectively. The mean serum calcium levels in Stages 1, 2, 3, 4, and 5 of CKD were 9.83±0.05, 9.79±0.20, 9.43±0.27, 8.72±0.38, and 7.8±0.10 mg/dL, respectively. The mean serum sodium levels in Stages 1, 2, 3, 4, and 5 of CKD were 137.33±0.57, 136.43±2.87, 136.92±2.15, 137.51±1.90, and 137.5±2.07 meq/L, respectively. Conclusion: There was significant rise in serum potassium and magnesium levels with decrease in eGFR in CKD patients. There was significant fall in serum calcium levels with decrease in eGFR in CKD patients. There was no significant correlation between serum sodium levels and eGFR in CKD patients

    Genome Characteristics Reveal the Biocontrol Potential of Actinobacteria Isolated From Sugarcane Rhizosphere

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    To understand the beneficial interaction of sugarcane rhizosphere actinobacteria in promoting plant growth and managing plant diseases, this study investigated the potential role of sugarcane rhizospheric actinobacteria in promoting plant growth and antagonizing plant pathogens. We isolated 58 actinobacteria from the sugarcane rhizosphere, conducted plant growth-promoting (PGP) characteristics research, and tested the pathogenic fungi in vitro. Results showed that BTU6 (Streptomyces griseorubiginosus), the most representative strain, regulates plant defense enzyme activity and significantly enhances sugarcane smut resistance by regulating stress resistance-related enzyme (substances (POD, PAL, PPO, TP) in sugarcane) activity in sugarcane. The genomic evaluation indicated that BTU6 has the ability to biosynthesize chitinase, b-1,3-glucanase, and various secondary metabolites and plays an essential role in the growth of sugarcane plants under biotic stress. Potential mechanisms of the strain in improving the disease resistance of sugarcane plants and its potential in biodegrading exogenous chemicals were also revealed. This study showed the importance of sugarcane rhizosphere actinobacteria in microbial ecology and plant growth promotion

    Sugarcane-Legume Intercropping Can Enrich the Soil Microbiome and Plant Growth

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    Soil microbes have a direct impact on plant metabolism and health. The current study investigates the comparative rhizobiome between sugarcane monoculture and sugarcane–soybean intercropping. A greenhouse experiment was performed with two treatments: (1) sugarcane monoculture and (2) sugarcane–soybean intercropped. We used a high-throughput sequencing (HTS) platform to analyze the microbial community. We used the 16S rRNA gene and internal transcribed spacer region primers to identify the microbial diversity. HTS results revealed that a total of 2,979 and 124 bacterial and fungal operational taxonomic units (OTUs) were observed, respectively.Microbial diversity results concluded that the intercropping system has a beneficial impact on soil microbes. The highest numbers of bacterial and fungal OTUs were found in the intercropping system, and these results also collaborated with quantitative PCR results. Additionally, intercropped sugarcane plants showed a higher weight of above- and below-ground parts than the monoculture. Soil chemical analysis results also complemented that the intercropping systemnourished organic carbon, total nitrogen, and soil enzyme activities. Correlation analysis of the diversity index and abundance concluded that soil nutrient content positively influenced the microbial abundance that improves plant growth. The present study frames out the profound insights of microbial community interaction under the sugarcane–soybean intercropping system. This information could help improve or increase the sugarcane crop production without causing any negative impact on sugarcane plant growth and development

    Transcriptome Analysis Reveals a Gene Expression Pattern That Contributes to Sugarcane Bud Propagation Induced by Indole-3-Butyric Acid

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    Sugarcane is a cash crop that plays an integral part in the sugar industry. The Sustainable Sugarcane Initiative (SSI) has been adopted globally, ensuring enough and aiming for more yield, helping increase disease-free sugarcane cultivation. Single-bud seeds could be the best approach for sugarcane cultivation. Indole-3-butyric acid (IBA) is a rooting agent utilized significantly in seedling propagation. Greenhouse experiment results discovered the significant growth promotion in sugarcane seedlings and accumulation of plant hormones at 100 ppm IBA. Next, we performed transcriptomic analysis of sugarcane buds using RNA sequencing and compared their gene expression during root development due to affect of IBA (100 ppm). A total of 113,475 unigenes were annotated with an average length of 836 bp (N50 = 1,536). The comparative RNA-seq study between the control (CK) and IBA-treated (T) buds showed significant differentially expressed unigenes (494 upregulated and 2086 downregulated). The IBA influenced major biological processes including metabolic process, the cellular process, and single-organism process. For cellular component category, cell, cell part, organelle, membrane, and organelle part were mainly affected. In addition, catalytic activity and binding were primarily affected in the molecular function categories. Furthermore, the expression of genes related to plant hormones and signaling pathways was analyzed by qRT-PCR, which was consistent with the RNA-seq expression profile. This study provides new insights into the IBA response to the bud sprouting in sugarcane based on RNA sequencing, and generated information could help further research on breeding improvement of sugarcane

    High-Throughput Sequencing-Based Analysis of Rhizosphere and Diazotrophic Bacterial Diversity Among Wild Progenitor and Closely Related Species of Sugarcane (Saccharum spp. Inter-Specific Hybrids)

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    Considering the significant role of genetic background in plant-microbe interactions and that most crop rhizospheric microbial research was focused on cultivars, understanding the diversity of root-associated microbiomes in wild progenitors and closely related crossable species may help to breed better cultivars. This study is aimed to fill a critical knowledge gap on rhizosphere and diazotroph bacterial diversity in the wild progenitors of sugarcane, the essential sugar and the second largest bioenergy crop globally. Using a high-throughput sequencing (HTS) platform, we studied the rhizosphere and diazotroph bacterial community of SaccharumofficinarumL. cv. Badila (BRS), Saccharumbarberi (S. barberi) Jesw. cv Pansahi (PRS), Saccharum robustum [S. robustum; (RRS), Saccharum spontaneum (S. spontaneum); SRS], and Saccharum sinense (S. sinense) Roxb. cv Uba (URS) by sequencing their 16S rRNA and nifH genes. HTS results revealed that a total of 6,202 bacteria-specific operational taxonomic units (OTUs) were identified, that were distributed as 107 bacterial groups. Out of that, 31 rhizobacterial families are commonly spread in all five species. With respect to nifH gene, S. barberi and S. spontaneum recorded the highest and lowest number of OTUs, respectively. These results were validated by quantitative PCR analysis of both genes. A total of 1,099 OTUs were identified for diazotrophs with a core microbiome of 9 families distributed among all the sugarcane species. The core microbiomes were spread across 20 genera. The increased microbial diversity in the rhizosphere was mainly due to soil physiochemical properties. Most of the genera of rhizobacteria and diazotrophs showed a positive correlation, and few genera negatively correlated with the soil properties. The results showed that sizeable rhizospheric diversity exists across progenitors and close relatives. Still, incidentally, the rhizosphere microbial abundance of progenitors of modern sugarcane was at the lower end of the spectrum, indicating the prospect of Saccharum species introgression breeding may further improve nutrient use and disease and stress tolerance of commercial sugarcane. The considerable variation for rhizosphere microbiome seen in Saccharum species also provides a knowledge base and an experimental system for studying the evolution of rhizobacteria-host plant association during crop domestication

    Clinical and Radiological Profile of Acute Cerebrovascular Accident With Special Emphasis On Neurosurgical and Medical Methods in Management of ICH

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    Introduction: Cerebrovascular accident  is a potentially lethal neurological disease of adult life. Intracerebral    hemorrhage is the  second most common subtype of stroke after ischemic stroke and accounts for approximately 10 % to 20 % of all strokes. Hypertension has been identified as the single most important risk factor causing intracerebral hemorrhage. Other risk factors include alcohol, smoking, diabetes, anticoagulant use and other genetic risk factors. The incidence of intracerebral hemorrhage increases with age and it is more among men and in Asians.  In acute stroke, CT scan readily distinguishes hemorrhage from infarct guiding anticoagulant therapy and site,  size of lesion provide prognostic value. Other stroke mimicking conditions can also be identified .TheICH score is a simple clinical grading scale that allows risk stratification on presentation with ICH. The role of surgery remains controversial in the management of acute intracranial hemorrhage.Material &Methods: The present study was carried out  in the Department of Medicine, Sanjay Gandhi Memorial Hospital, Rewa between  April 2019 to June 2020 (15months). A total of  200  patients diagnosed with stroke were selected for the study.Observations and Results: CVA is most commonly found among elderly patients. The most common clinical features was found to be weakness(47%) .In the present study most common type of stroke was ischemic stroke(63%) followed by hemorrhagic(23%).In the present study most common site of Ishemic stroke was internal capsule region (40.47%), followed by parietal lobe(20.63%) . The radiographic findings were favoring the middle cerebral artery territory involvement. In the present study, mortality for patients with an ICH score of 1,2,3,4 and 5 were 27.27%, 44.4%, 66.6%, 100%, 100% respectively. In the present study there was no significant difference between outcome of patients who were managed by neurosurgery and medical management
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