A simple reversed phase High Performance Liquid Chromatography method development and validation for determination of Carvedilol in pharmaceutical dosage forms

A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the estimation of Carvedilol in pharmaceutical preparations. Chromatographic determination was performed on a reversed phase C 18 column (4.5 mm x 250 mm; 5 m particle size) using a mixture of Phosphate buffer: Acetonitrile (65:35) as mobile phase at a flow rate of 1ml/min with UV detection at 240 nm. The method was validated for linearity, accuracy, repeatability, precision, reproducibility, and specificity as per International ICH guidelines. The method was also used in determination Carvedilol content in five commercial brands available in Bangladeshi market. The method was linear in the range between 5 35 g/ml, exhibited good correlation coefficient (R 2 = 0.998) and good Accuracy study (98.08 %-99.91%). The method was found to specific for Carvedilol in presence of common excipients. Statistical analysis performed with proposed method proved it to be precise, accurate and reproducible. Hence it can be employed for routine analysis of Carvedilol both in bulk and commercial formulations

Development and validation of a simple UV spectrophotometric method for the determination of levofloxacin both in bulk and marketed dosage formulations

A rapid, specific and economic UV spectrophotometric method has been developed using a solvent composed of water:methanol:acetonitrile (9:0.5:0.5) to determine the levofloxacin content in bulk and pharmaceutical dosage formulations. At a pre-determined λmax of 292 nm, it was proved linear in the range of 1.0â12.0 μg/mL, and exhibited good correlation coefficient (R2=0.9998) and excellent mean recovery (99.00â100.07%). This method was successfully applied to the determination of levofloxacin content in five marketed brands from Bangladesh and the results were in good agreement with the label claims. The method was validated statistically and by recovery studies for linearity, precision, repeatability, and reproducibility. The obtained results proved that the method can be employed for the routine analysis of levofloxacin in bulks as well as in the commercial formulations. Keywords: Fluoroquinolone, Levofloxacin, UV spectrophotometric method, Validatio