Дослідження історії книжкової культури України

У статті розкрито основні наукові напрями та завдання Інституту книгознавства Національної бібліотеки України ім. В.І. Вернадського. Обґрунтовано ідею обрання та шляхи і форми виконання наукової теми Інституту на 2016—2018 рр. «Історія книжкової культури в Україні», суть якої — через дослідження конкретних книжкових, образотворчих, музичних, картографічних пам’яток та історичних бібліотечних зібрань створити переконливу картину історії вітчизняної книжкової культури.В статье раскрыта цель и основные задачи Института книговедения Национальной библиотеки Украины им. В.И. Вернадского. Обоснована идея выбора и форм выполнения научной темы Института на 2016—2018 гг. «История книжной культуры в Украине», суть которой — через исследования конкретных книжных, изобразительных, музыкальных, картографических памятников и исторических библиотечных собраний создать убедительную картину истории отечественной книжной культуры.The article deals with the purpose and the main problems in the scientific activity of the Institute of Book Science of Vernadsky National Library of Ukraine. The conception of the Institute’s scientific theme “The history of the book culture in Ukraine” and forms of its fulfillment are elucidated. The main task of the research is through the investigations of concrete book, graphic, musical, cartographic monuments and historical library collections to create a persuasive view of the history of the national book culture

État des réalisations au 31 décembre 1988 sur le projet protection de la Kibira

This paper compares the performance of several models forecasting travel time variability for road traffic, using before/after data from the introduction of the Stockholm congestion charges. Models are estimated on before-data, and the models’ forecasts for the after-situation are compared to actual after measurements. The accuracy of the models vary substantially, but several models are able to forecast the benefits from reduced travel time variability with sufficient accuracy to make them useful for decision making

Diversity and catalytic potential of PAH-specific ring-hydroxylating dioxygenases from a hydrocarbon-contaminated soil.

International audienceRing-hydroxylating dioxygenases (RHDs) catalyze the initial oxidation step of a range of aromatic hydrocarbons including polycyclic aromatic hydrocarbons (PAHs). As such, they play a key role in the bacterial degradation of these pollutants in soil. Several polymerase chain reaction (PCR)-based methods have been implemented to assess the diversity of RHDs in soil, allowing limited sequence-based predictions on RHD function. In the present study, we developed a method for the isolation of PAH-specific RHD gene sequences of Gram-negative bacteria, and for analysis of their catalytic function. The genomic DNA of soil PAH degraders was labeled in situ by stable isotope probing, then used to PCR amplify sequences specifying the catalytic domain of RHDs. Sequences obtained fell into five clusters phylogenetically linked to RHDs from either Sphingomonadales or Burkholderiales. However, two clusters comprised sequences distantly related to known RHDs. Some of these sequences were cloned in-frame in place of the corresponding region of the phnAIa gene from Sphingomonas CHY-1 to generate hybrid genes, which were expressed in Escherichia. coli as chimerical enzyme complexes. Some of the RHD chimeras were found to be competent in the oxidation of two- and three-ring PAHs, but other appeared unstable. Our data are interpreted in structural terms based on 3D modeling of the catalytic subunit of hybrid RHDs. The strategy described herein might be useful for exploring the catalytic potential of the soil metagenome and recruit RHDs with new activities from uncultured soil bacteria

Plan Directeur Forestier National. Mission préparatoire

Cette mission prépare le démarrage de l'inventaire des connaissances, des documents et des actions du secteur forestier. Des réunions ont permis de recueillir les réactions des participant