Utilization of Guarango (Caesalpinia spinosa) in the Province of Loja

Guarango (Caesalpinia spinosa) was studied in different parts of Loja province, mainly Loja, Catamayo, Gonzanamá, and Paltas, to use this species in the food and chemical industries because many studies in our country show that this species is not correctly used. It started with studies of the yields according to the place and time of recollection, after which the correct treatment was found, like the kind of grinding to optimize the handling of the sample, the chemical determination (tannins) in the pod, and its bromatological characteristics (moisture, fat, and protein). The chemical determination of tannins was done through titration of a macerated pod using carmine indigo and KMnO4 to 0,1 N, while bromatological determination with moisture, fat, and protein was applied using the standard methods of AOAC 7003, AOAC 7.056 y AOAC 47.021, respectively. In these results, it was found that there is little variability in experiments of percent of integument, endosperm, and germ, while in experiments of moisture, the amount of pod and seeds had significant differences. In addition, the percentage of tannins was excellent for use in the leather industry. While the germen had qualities of a good food supplement due to its high fat and protein content and low moisture. Finally, a method of extraction of the guarango parts could be designed that can be easily replicable by farmers. Based on the results, we can conclude that it is possible to establish ventures with guarango (C. spinosa) due to the remarkable properties of this species in food, textiles, and other industries. Keywords: Caesalpinia spinosa, guarango, pod, germ, used. Resumen Se realizó un estudio sobre el guarango (Caesalpinia spinosa) de diferentes cantones de la provincia de Loja, principalmente: Loja, Catamayo, Gonzanamá y Paltas, con la finalidad de aprovechar esta especie ofreciendo alternativas de uso dentro de la industria química y alimentaria ya que, según estudios realizados, dentro de nuestro país no es aprovechada. Se partió desde el estudio de rendimientos según el lugar y tiempos de recolección, posterior a esto se buscó el tratamiento adecuado como molienda para el manejo óptimo de la muestra, la determinación química (taninos) en vaina y bromatológica (humedad grasa y proteína). Para la determinación química (taninos) se realizó titulación al macerado de la vaina usando índigo carmín y KMnO4 al 0,1 N, mientras que para determinación bromatológica como la humedad, grasa y proteína se aplicó métodos estandarizados de la AOAC 7003, AOAC 7.056 y AOAC 47.021, respectivamente. En los resultados se obtuvo ensayos que demostraron la poca variabilidad del porcentaje de tegumento, endospermo y germen, mientras que en los ensayos de humedad, cantidad de vainas y semillas, taninos, hay variabilidades significativas

Main Polar Metabolites from Leaves of the Native Andean Species Jungia rugosa Less (Asteraceae)

The ethanolic extract of Jungia rugosa Less, a popular Andean species belonging to the family Asteraceae, was characterized chemically. The extract was deprived of chlorophyll by solid-phase extraction, using Diaion Hp-20 resin as solid phase, and eluting with a mixture of EtOH/H2O according to a decreasing polarity gradient. The chlorophyll-free extract was then repeatedly fractionated by open column chromatography in normal phase and preparative thin layer chromatography. Two main metabolites were finally purified and identified through structure elucidation. Nuclear magnetic resonance spectroscopy (NMR) and electrospray ionization-mass spectrometry (ESI-MS) were the 2 techniques used for analysis. The identified metabolites were coumarins: (1) umbelliferone and (2) coumarin-5- methyl-4α-glucoside. Compound 1 is known in the literature for having anti-inflammatory, antihyperglycemic, and antitumor activities. Compound 2 has not been described before for this botanical genus and its pharmacological effects are still uninvestigated. Keywords: Jungia rugosa, umbelliferone, coumarin-5-methyl-4-glucoside, NMR, ESI-MS, Ecuador. Resumen Se caracterizó químicamente el extracto etanólico de Jungia rugosa Less, una popular especie andina perteneciente a la familia Asteraceae. Se eliminó las clorofilas al extracto mediante extracción en fase sólida, utilizando como fase sólida resina de Diaion Hp-20 y eluyendo con una mezcla de EtOH/H2O según un gradiente de polaridad decreciente. El extracto libre de clorofila se fraccionó repetidamente mediante cromatografía en columna abierta en fase normal y cromatografía en capa fina preparativa. Finalmente, se purificaron e identificaron dos metabolitos principales mediante la elucidación de la estructura. Se utilizaron espectroscopía de resonancia magnética nuclear (RMN) y espectrometría de masas de ionización por aspersión de electrones (ESI-MS). Los metabolitos identificados fueron las cumarinas: umbeliferona (1) y cumarina-5-metil-4α-glucósido (2). El compuesto 1 es conocido en la literatura por tener actividad antiinflamatoria, antihiperglucémica y antitumoral. El compuesto 2 no se ha descrito antes para este género botánico y sus efectos farmacológicos aún no se han investigado. Palabras Clave: Jungia rugosa, umbeliferona, cumarina-5-metil-4-glucósido, RMN, ESI-MS, Ecuador

Semi-synthetic Derivatives of Limonin A: Synthesis Using Basic Hydrolysis and Reduction with NaBH4

Limonin A (L-A) was obtained from seeds of Citrus sinensis that were dried, grounded, and extracted using Soxhlet equipment. Initially, 100% hexane was used to eliminate fats, and finally, 100% dichloromethane was used in order to concentrate and isolate the compound. L-A recrystallization was performed using acetic acid. The purified fraction was identified as limonin according to spectral nuclear magnetic resonance (NMR) data. Two reactions were performed from L-A: Basic hydrolysis that acted on the A’ lactone ring producing a sodium salt of the lactone, and reduction with NaBH4 to reduce the carbonyl of carbon C-7 and generate the corresponding alcohol limonol. The structural modification of abundant secondary metabolites can become a source of new products with biological activity, useful in the pharmaceutical, cosmetic, and agrochemical industries of natural origin. Keywords: limonin, semisynthetic derivates, bioactive compounds, non-timber forest products. Resumen La limonina A (L-A) se obtuvo a partir de semillas de Citrus sinensis, que fueron secadas, molidas y extraídas en equipo Soxhlet, utilizando inicialmente hexano al 100% para eliminar grasas y finalmente diclorometano al 100% para concentrar y aislar el compuesto. La re cristalización de la L-A se realizó utilizando ácido acético. La fracción purificada se identificó como limonina, acorde a los datos espectrales de RMN (Resonancia Magnética Nuclear). A partir de la Limonina A se realizaron dos reacciones: Hidrólisis básica que actuó sobre el anillo de lactona A’ produciendo la sal sódica de lactona y reducción con NaBH4 para reducir el carbonilo del carbono C-7 y generar el correspondiente alcohol limonol. La modificación estructural de metabolitos secundarios abundantes puede convertirse en una fuente para generar nuevos productos con actividad biológica, útiles en las industrias farmacéutica, cosmética y agroquímica de origen natural. Palabras Clave: Limonina; derivados semisintéticos, compuestos bioactivos, productos forestales no maderables

Una mirada al origen y las propiedades de los suelos magnésicos en el valle del cauca-colombia

Se estudiaron las relaciones Si/Mg y Ca/Mg, en tres transectos y 12 perfiles de suelos, con lluvias inferiores a 1400 mm anuales. Más de 15% de minerales ferromagnesianos en la fracción arena fueron garantía para la aparición de contenidos de Mg+2 mínimos de 5 Cmol (+)/kg en todos los paisajes. Variaciones anuales de la precipitación entre 800 y 1400 mm no influyeron en la variabilidad del magnesio de 5 a 41 Cmol (+)/kg. El Mg+2 aumentó en el sentido en que decreció la tasa de infiltración promedia. Los suelos con arcillas oxídicas acumularon SO4=, yeso en los poros y adolecieron de horizontes Bk; con influencia de piroclastos aumentaron Na+. Cuando presentaron Si / Mg and gt; 5 tuvieron menores concentraciones relativas de Mg+2 y Ca+2, excepto en familias esmectítica y caolinítica. Las aguas de riego presentaron C.E. and lt; 1 dS/m, la mayoría carbonato de sodio residual (CSR), y para casi todas se prevén aportes de magnesio al suelo. Hubo efecto específico en todos los paisajes; para Ca/Mg and lt; 2 se atenuó cuando el contenido de materia orgánica fue similar a 5% o con mineralogías caoliníticas; con mineralogías micáseas el efecto se dio desde relaciones Ca/Mg 2-3. Valores de Ca+2 and gt; 10 Cmol (+)/kg y pH ­ 7.5 inhibieron la acumulación de Na+. ABSTRACT A sigh over the origin and properties of magnesic soils in Valle del Cauca-Colombia. They were studied as a function of Si/Mg and Ca/Mg relations in three transect and 12 soil profiles where rain precipitation was lower than 1400 mm. More than 15% of ferromagnesian minerals in sand fraction allows at least a minimum Mg+2 content of 5 Cmol (+)/kg. Annual rain variations between 800 and 1400 mm had not influence in Mg+2 variability between 5 to 41 Cmol(+)/kg. Mg+2 contents had a tendency to increase as well as medium infiltration rate decrease. Magnesic soils with oxidic clays in general allowed accumulation of S04= ion, gypsum in pore space, and lacking of Bk horizons; but with piroclastic materials Na+ tended to increase. When showed Si / Mg and gt; 5, had relatively less Mg+2 y Ca+2 concentrations except in smectitic and kaolinitic families. All irrigation waters had C.E and lt; 1 dS/m, most of them residual sodium carbonate (RSC), and most of times they foresee high apports of magnesium to soils. There were specific effect in all landscapes; for Ca/Mg and lt; 2 was lessen when soil organic matter content was close to 5%, or if kaolinitic mineralogy; with micaceous mineralogy it occurred for Ca/Mg of 2-3. Ca+2 values greater than 10 Cmol (+)/kg and pH ­ 7.5 suppressed soil Na+ accumulation. Key words: magnesic soils, Mg specific effect, irrigation water quality

Localization of gravity on a de Sitter thick braneworld without scalar fields

In this work we present a simple thick braneworld model that is generated by an intriguing interplay between a 5D cosmological constant with a de Sitter metric induced in the 3-brane without the inclusion of scalar fields. We show that 4D gravity is localized on this brane, provide analytic expressions for the massive Kaluza-Klein (KK) fluctuation modes and also show that the spectrum of metric excitations displays a mass gap. We finally present the corrections to Newton's law due to these massive modes. This model has no naked singularities along the fifth dimension despite the existence of a mass gap in the graviton spectrum as it happens in thick branes with 4D Poincare symmetry, providing a simple model with very good features: the curvature is completely smooth along the fifth dimension, it localizes 4D gravity and the spectrum of gravity fluctuations presents a mass gap, a fact that rules out the existence of phenomenologically dangerous ultralight KK excitations in the model. We finally present our solution as a limit of scalar thick branes.Comment: 11 pages in latex, no figures, title and abstract changed, a new section and some references adde

Sexual Size Dimorphism and Body Condition in the Australasian Gannet

Funding: The research was financially supported by the Holsworth Wildlife Research Endowment. Acknowledgments We thank the Victorian Marine Science Consortium, Sea All Dolphin Swim, Parks Victoria, and the Point Danger Management Committee for logistical support. We are grateful for the assistance of the many field volunteers involved in the study.Peer reviewedPublisher PD

Efectividad y seguridad comparada de inhibidores del cotransportador sodio-glucosa tipo 2 (iSGLT2) en diabetes mellitus tipo 2: revisión rápida de revisiones sistemáticas y metaanálisis

Objetivo: recopilar y evaluar la evidencia disponible respecto a la efectividad y seguridad comparada de inhibidores del cotransportador de sodio glucosa tipo 2 (iSGLT2) en pacientes con diabetes mellitus tipo 2. Metodología: se realizó una revisión sistemática rápida de revisiones sistemáticas de iSGLT2 en las bases de datos Medline y Embase hasta septiembre de 2019. El desenlace primario cardiovascular incluyó eventos adversos cardiovasculares mayores (MACE) que corresponden a muerte por causa cardiovascular, ataque cerebrovascular (ACV) no fatal, infarto agudo de miocardio (IAM) no fatal, además de hospitalización por insuficiencia cardíaca; desenlace renal (definido como progresión de enfermedad renal), disminución de la tasa de filtración glomerular (TFG) y de la relación albuminuria-creatinuria. El desenlace de seguridad agrupó hipoglucemia, fracturas, infecciones urinarias, entre otros. La calidad metodológica de las revisiones se evaluó con el instrumento A measurement Tool to Assess Systematic Review (AMSTAR-2) modificado. Resultados: se incluyeron 5 revisiones sistemáticas de la literatura de calidad media y alta según AMSTAR-2 modificado, entre las cuales se encontró que los iSGLT2 reducen el riesgo de mortalidad cardiovascular en un 23 %, de mortalidad por todas las causas en 20 % y en admisión hospitalaria por insuficiencia cardíaca en un 33 %, en comparación con el cuidado estándar. En cuanto al desenlace renal, los iSGLT2 enlentecen el deterioro de la enfermedad renal y reducen la progresión a albuminuria en pacientes con proteinuria ya documentada. En el desenlace de seguridad se observa una mayor posibilidad de desarrollar infecciones del tracto genitourinario respecto a antidiabéticos orales. Conclusiones: la evidencia sugiere que los iSGLT2 son efectivos en la reducción del riesgo de mortalidad cardiovascular, de mortalidad por todas las causas, de admisión hospitalaria por insuficiencia cardíaca, de progresión de la nefropatía y del desarrollo de enfermedad renal en estadio final. En desenlaces de seguridad, la evidencia sugiere que los iSGLT2 tienen menor riesgo de eventos de hipoglucemia

Main belt asteroids taxonomical information from dark energy survey data

While proper orbital elements are currently available for more than 1 million asteroids, taxonomical information is still lagging behind. Surveys like SDSS-MOC4 provided preliminary information for more than 100 000 objects, but many asteroids still lack even a basic taxonomy. In this study, we use Dark Energy Survey (DES) data to provide new information on asteroid physical properties. By cross-correlating the new DES data base with other data bases, we investigate how asteroid taxonomy is reflected in DES data. While the resolution of DES data is not sufficient to distinguish between different asteroid taxonomies within the complexes, except for V-type objects, it can provide information on whether an asteroid belongs to the C- or S-complex. Here, machine learning methods optimized through the use of genetic algorithms were used to predict the labels of more than 68 000 asteroids with no prior taxonomic information. Using a high-quality, limited set of asteroids with data on gri slopes and i - z colours, we detected 409 new possible V-type asteroids. Their orbital distribution is highly consistent with that of other known V-type objects

The PSZ-MCMF catalogue of Planck clusters over the des region

We present the first systematic follow-up of Planck Sunyaev–Zeldovich effect (SZE) selected candidates down to signal-to-noise (S/N) of 3 over the 5000 deg2 covered by the Dark Energy Survey. Using the MCMF cluster confirmation algorithm, we identify optical counterparts, determine photometric redshifts, and richnesses and assign a parameter, fcont, that reflects the probability that each SZE-optical pairing represents a random superposition of physically unassociated systems rather than a real cluster. The new PSZ-MCMF cluster catalogue consists of 853 MCMF confirmed clusters and has a purity of 90 per cent. We present the properties of subsamples of the PSZ-MCMF catalogue that have purities ranging from 90 per cent to 97.5 per cent, depending on the adopted fcont threshold. Halo mass estimates M500, redshifts, richnesses, and optical centres are presented for all PSZ-MCMF clusters. The PSZ-MCMF catalogue adds 589 previously unknown Planck identified clusters over the DES footprint and provides redshifts for an additional 50 previously published Planck-selected clusters with S/N>4.5. Using the subsample with spectroscopic redshifts, we demonstrate excellent cluster photo-z performance with an RMS scatter in Δz/(1 + z) of 0.47 per cent. Our MCMF based analysis allows us to infer the contamination fraction of the initial S/N>3 Planck-selected candidate list, which is ∼50 per cent. We present a method of estimating the completeness of the PSZ-MCMF cluster sample. In comparison to the previously published Planck cluster catalogues, this new S/N>3 MCMF confirmed cluster catalogue populates the lower mass regime at all redshifts and includes clusters up to z∼1.3

Chemical Analysis of the Brightest Star of the Cetus II Ultra-Faint Dwarf Galaxy Candidate

We present a detailed chemical abundance analysis of the brightest star in the ultra-faint dwarf (UFD) galaxy candidate Cetus II from high-resolution Magellan/MIKE spectra. For this star, DES J011740.53-173053, abundances or upper limits of 18 elements from Carbon to Europium are derived. Its chemical abundances generally follow those of other UFD galaxy stars, with a slight enhancement of the alpha-elements (Mg, Si, and Ca) and low neutron-capture element (Sr, Ba, Eu) abundances supporting the classification of Cetus II as a likely UFD. The star exhibits lower Sc, Ti, and V abundances than Milky Way (MW) halo stars with similar metallicity. This signature is consistent with yields from a supernova (SN) originating from a star with a mass of ~11.2 solar masses. In addition, the star has a Potassium abundance of [K/Fe] = 0.81 which is somewhat higher than the K abundances of MW halo stars with similar metallicity, a signature which is also present in a number of UFD galaxies. A comparison including globular clusters (GC) and stellar stream stars suggests that high K is a specific characteristic for some UFD galaxy stars and can thus be used to help classify objects as UFD galaxies.Comment: 15 pages, 7 figures, 5 tables, accepted to Ap