Isolation and Identification of Lactic Acid Bacteria Strains and Their Effects on the Fermentation Quality of Elephant Grass (\u3ci\u3eCenchrus purpureus\u3c/i\u3e) Silage

This study aims to isolate and identify lactic acid bacteria and examine their effects on the fermentation quality of elephant grass silage. The isolated strains were identified based on morphological, physiological and biochemical characteristics as well as 16S rRNA analysis. Three strains namely Pediococcus acidilactic (AZZ5), Lactobacillus plantarum subsp. Plantarum (AZZ4), Lactobacillus plantarum subsp. Argentoratensis (AZZ6) were isolated from elephant grass silage. Isolation of the microbes was done by serial dilution method. Three LAB and one commercial bacteria Lactobacillus Plantarum, Ecosyl MTD/1(CB)) were used as additives to fresh material of elephant grass. To follow the fermentation quality during ensiling, samples were taken on days 30, 60 and 90 of ensiling for chemical analysis. The strain AZZ5 was identified as Pediococcus genus while AZZ4 and AZZ6 were Lactobacillus genus. Compared to the control, all the isolates improved the silage quality of elephant grass silage. In conclusion, AZZ4 performed better among all inoculants

Effect of Microbial Inoculant on Fermentation Period and Chemical Composition of Sweet Sorghum Silage

Sorghum is one of the most suitable plants for silage production and becoming an increasingly important forage crop in many regions of the world. Due to its high water-soluble carbohydrates contents (WSC) and low buffer capacity, it is easy to ensile. This study evaluates the effect of adding Lactobacillus plantarum AZZ4 and Pediococcus acidilactici AZZ5 isolated from elephant grass on the fermentation period and chemical composition of sweet sorghum silage. Two strains of Lactobacillus plantarum subsp. Plantarum (AZZ4) , Pediococcus acidilactici (AZZ5), and one commercial bacteria Lactobacillus Plantarum, Ecosyl MTD/1(CB)) were chosen as additives at 6 log colony forming units (cfu)/g of fresh sweet sorghum grass in laboratory silos (1000g). Silos for each treatment were opened after 15,30, and 60d, respectively. Compared to the control, all the isolates improved the silage quality of sweet sorghum silage, indicated by significantly (P \u3c 0.05) lower ammonia nitrogen (NH3-N) content and undesirable microorganism counts, and higher lactic acid (LA) contents and ratios of lactic acid/acetic acid (LA/AA). During ensiling, AZZ4 performed better among all inoculants, indicated by a significantly (P \u3c 0.05) decrease in pH and ammonia- N contents and a higher increase in lactic acid contents

Review: Potential of using lactic acid bacteria as inoculant for seaweed silage towards sustainable aquaculture

Aquaculture is an essential sector in Malaysian fisheries and plays a significant role in the national economy. Organic pollution, opportunistic microorganisms in aquaculture farms, and international contamination of feed by mycotoxigenic fungi are serious issues. Lactic acid bacteria are a biological method for maintaining and restoring the normal physiological state and increasing productivity. However, research into the effects of inoculated seaweed silage with lactic acid bacteria inoculants is limited and has been conducted under various experimental conditions. The aim of this review is the potential of using lactic acid bacteria as an inoculant for seaweed silage for the development of sustainable aquaculture. According to the microbiological point of view, the results in this area are weak, and limited information is available. Due to its high nutritional content, seaweed silage is a promising feed ingredient and is gaining popularity as an alternative feed. Seaweeds contain valuable metabolites such as polyunsaturated fatty acids, carotenoids, phlorotannins, carrageenan, alginate pigments, agar, and minerals (manganese, iodine, calcium, iron, selenium, sodium, zinc) are natural antibiotic source in aquaculture feed. In conclusion, increasing the use of effective lactic acid bacteria as an inoculant in aquaculture feed can make the aquacultural sector more productive, safer, and friendly to human and environment then contributing to the long-term development of aquaculture