Differential effects of epigallocatechin gallate on cigarette smoke-induced upregulation of CINC-1 and IL-6 in Rat H9c2 cardiomyocytes

INTRODUCTION: Cigarette smoke (CS) containing numerous harmful substances is considered to precipitate spasm of micro vessels, which is supported as a cause of idiopathic-dilated cardiomyopathy, as well as have a toxic effect on the myocardium. The mechanism is currently unclear, but both oxidative stress and inflammatory responses may play an essential role in the CS-induced biological processes. Several antioxidant agents have been used in the control of the inflammatory responses. The aim of this study was to investigate the effect of epigallocatechin gallate (EGCG), the major component of polyphenols in green tea, on CS-induced inflammatory responses in rat H9c2 cardiomyocyte …published_or_final_versio

Intermittent hypoxia-induced endothelial activation in EA.hy926 cells in vitro

INTRODUCTION: Obstructive sleep apnoea is characterised by repetitive episodes of complete or partial upper airway collapse during sleep, leading to recurrent drop in arterial oxygenation (intermittent hypoxia [IH]) and sleep fragmentation. Repetitive IH with rapid reversal to normoxia initiates a series of pathological events such as oxidative stress, inflammation, and sympathetic activation, all of which contributes to endothelial dysfunction (a predictor and precursor of atherosclerosis). The aim of the current study was to investigate the effect of IH on the activation of endothelial cells in a cellular model in …published_or_final_versio

Effects of cigarette smoke on 5-hydrotrytamine metabolism in human bronchial epithelial cells

INTRODUCTION: Serotonin (5-hydrotrytamine, 5-HT) plays an important role in pulmonary functions. It is synthesised from tryptophan by aromatic 1-amino acid decarboxylase (AADC) and released at the sites, which binds to 5-HT receptors at the postsynaptic ends. Excess or turnover 5-HT will be recycled back into cells via 5-HT reuptake transporter (SERT), stored or subsequently metabolised by monoamine oxidase A (MAO-A) inside the cells. With the influence of smoking on serotoninergic neurons, we hypothesised that there may be a potential 5-HT analogue in cigarette smoke. We aimed to study the effect of 5-HT on interleukin (IL)–8 release and the effects of cigarette smoke on the metabolism of 5-HT in human bronchial epithelial …published_or_final_versio

Role of heme oxygenase-1 in intermittent hypoxia-induced inflammation and oxidative stress in eAhy 926 endothelial cell line

published_or_final_versionThe 16th Medical Research Conference (MRC), Department of Medicine, The University of Hong Kong, Hong Kong, China, 22 January 2011. In Hong Kong Medical Journal, 2011, v. 17 n. 1, suppl. 1, p. 29, abstract no. 4

Ketanserin attenuates cigarette-mediated oxidative stress in human bronchial epithelial cells

published_or_final_versionThe 15th Medical Research Conference, Department of Medicine, The University of Hong Kong, Hong Kong, 16 January 2010. In Hong Kong Medical Journal, 2010, v. 16, suppl. 1, p. 34, abstract no. 5

Cellular mechanisms involved in intermittent hypoxia-induced heart damage in rat

Poster PresentationBACKGROUND: Obstructive sleep apnoea (OSA), characterised by intermittent hypoxia (IH) during sleep, is increasingly recognised as an independent risk factor of cardiovascular diseases (CVD). OSA has been reported to be associated with changes in the levels of circulating oxidative stress and inflammatory markers as well as dyslipidemia, supporting their mediating roles in cardiovascular pathogenesis. This study aimed to investigate the ...published_or_final_versionThe 17th Medical Research Conference, Department of Medicine, The University of Hong Kong, Hong Kong, 14 January 2012. In Hong Kong Medical Journal, 2012, v. 18 n. 1, suppl. 1, p. 23, abstract no. 2

Suppression of inflammation during adipogenic differentiation in human preadipocytes

INTRODUCTION: Preadipocytes are present in adipose tissues throughout adult life that can differentiate into mature adipocytes in response to environmental factors. However, little is known about the secretory inflammation-associated cytokines / chemokines during adipogenic differentiation. METHODS: Human preadipocytes-subcutaneous (HPA-s) cells were cultured to 80% confluence. From preadipocytes to mature adipocytes, a total of six induction cycles …published_or_final_versio

Modification of serum adiponectin and CINC-1 levels by intermittent hypoxia and/or hyperlipidemia in vivo

published_or_final_versionThe 15th Medical Research Conference, Department of Medicine, The University of Hong Kong, Hong Kong, 16 January 2010. In Hong Kong Medical Journal, 2010, v. 16, suppl. 1, p. 22, abstract no. 2

Effects of intermittent hypoxia on A-/E-FABP expression in human aortic endothelial cells

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Age-dependent alterations of cigarette smoke-induced oxidative and inflammatory responses in rats

Oral PresentationBACKGROUND: Cigarette smoking (CS) is a leading cause of chronic obstructive pulmonary disease (COPD). The prevalence of COPD is much higher among the elderly. However, the impact of early-age CS exposure on CS induced COPD is unknown. This study aimed to investigate whether CS-induced oxidative and inflammatory responses were ...published_or_final_versionThe 17th Medical Research Conference, Department of Medicine, The University of Hong Kong, Hong Kong, 14 January 2012. In Hong Kong Medical Journal, 2012, v. 18 n. 1, suppl. 1, p. 39, abstract no. 5