Morphological identification of haematopoietic cells in pronephros of common carp (Cyprinus carpio Linnaeus, 1758)

Haemopoietic tissue of the common carp pronephros (Cyprinus carpio Linnaeus, 1758) was studied in regard of morphometric analysis of the erythropoietic, leukopoietic and thrombopoietic cell populations. The aim of this study was to perform evaluation of immature precursor cells in head kidney of common carp (sampled from Bardaca lake) because there is no well known data regarding this issue. Microscopic identification of haematopoietic cells included measurement of cell and nuclear size, nuclear-cytoplasmic ratio, determination of the cell and nuclear shape, cytosol coloration and presence of specific granules in the cytosol. The frequency of immature cells and their cell area was also analyzed. Erythroblasts were the most abundant among all observed haemopoietic cell lineages and were the most variable in the size. The largest area was characteristic of monocyte precursors and no significant differences were observed regarding the cell area between prothrombocytes and lymphoblasts, which makes difficult in cell characterization. High number of emerged cells in short time also makes difficult to identify particular stages of maturation in some bloodlines. Rapid maturation of granuloid cells observed within the haemopoietic tissue indicates their functional significance in adaptation to the changeable microenvironment

LONG-TERM TRENDS IN THE STRUCTURE AND DYNAMICS OF THE FISH COMMUNITIES IN BUŠKO BLATO RESERVOIR

Akumulacija Buško Blato, treća najveća hidroakumulacija u Europi, smještena je u jugoistočnom dijelu krškog Livanjskog polja. Ovaj vodeni ekosustav odlikuje se vrlo bogatom ihtiofaunom i prisutnošću četiri endemske vrste riba. Ihtiološka istraživanja navedene akumulacije bila su u fokusu brojnih istraživanja. Ihtiofauna Buškog Blata bila je izložena promjenama različitih ekoloških čimbenika, što se odrazilo na strukturu i sastav ribljih populacija. Glavni cilj ovog rada bio je procijeniti trenutno stanje i predvidjeti buduće trendove u strukturi i dinamici ihtiofaune na temelju prikupljenih terenskih podataka i sveobuhvatnih analiza iz literaturnih podataka. Rezultati istraživanja pokazali su prisutnost 11 vrsta riba iz četiri porodice, što je najveći broj vrsta riba ikada zabilježenih u ovom ekosustavu. Sander lucioperca, Lepomis gibbosus, Pseudorasbora parva, i Tinca tinca prvi su put zabilježeni u ovom ekosustavu, dok neke prethodno zabilježene vrste nisu pronađene. Rezultati analiza jasno ukazuju na prisutnost prirodne interspecifične konkurencije i značajnu razinu ugroženosti endemskih vrsta riba uzrokovanu ljudskim aktivnostima.Buško Blato Reservoir, the third largest hydro accumulation in Europe, is situated in the south-eastern part of the karstic Livanjsko Polje Valley. This aquatic ecosystem is distinguished by a very rich ichthyofauna and the presence of four endemic fish species. Numerous studies focus on ichthyological researches of Buško Blato Reservoir. Its ichthyofauna has been exposed to changes in ecological factors, which in turn reflected on the structure and composition of fish populations. The main objective of this paper was to assess the current state and predict future trends in the ichthyofauna structure and dynamics based on the field data and comprehensive analyses of literature data. The results of the research indicated the presence of 11 fish species from four families, which is the largest number of fish species ever recorded in this ecosystem. Sander lucioperca, Lepomis gibbosus, Pseudorasbora parva and Tinca tinca were recorded for the first time in this ecosystem, while some previously recorded species were not found. The results of the analyses clearly indicate the presence of natural interspecific competition and significant level of threats to the endemic fish species caused by human activities

EFFECT OF HYPERTHERMIA ON DIFFERENTIAL BLOOD CELL COUNT IN RATS (Rattus norvegicus)

<p>We investigated the impact of short-term experimental hyperthermia on differential blood cell count in rats Rattus norvegicus (male and female Wistar strain). The blood used for the analysis was obtained by the method of direct puncture of the heart. We analyzed the forms of leukocytes (granulocytes, lymphocytes and monocytes). The effect of short-term hyperthermia is statistically significant increase in lymphocyte count, and decrease in monocyte count. The changes in other leukocyte count are not statistically significant.</p><p>Key words: hyperthermia, differential blood cell count</p

HEMATOLOGICAL STATUS OF THE RAT RATTUS NORVEGICUS IN HYPOTHERMIA

We investigated erythrogram and leukogram in the rats Rattus norvegicus (Wistar strain) during the short-term hypothermia under the laboratory controlled conditions. Control group of animals was anesthetized using diethylether and then heart punction was performed. Experimental group of animals was exposed to short-term hypothermia by "a'frigore" method. Once the body temperature of experimental animals decreased (rectal temperature was 15 °C), we performed punction of the heart. Hematological status was determined by the following parameters: erythrogram (red blood cell count, hematocrit (Hct), hemoglobin (Hb), MCV, MCH and MCHC) and leukogram (leukocytes, segmented and non-segmented neutrophils, eosinophilic and basophilic granulocytes, lymphocytes and monocytes). Under hypothermic conditions, a significant decrease in neutrophilic granulocytes and increase in lymphocytes were noted. The red blood cell count and hemoglobin concentrations were significantly increased while the values of MCV and MCH decreased in hypothermia. Key words: hypothermia, erythrogram, leukogra

Long-Term Trends in the Structure and Dynamics of the Fish Communities in Buško Blato Reservoir

Buško Blato Reservoir, the third largest hydro accumulation in Europe, is situated in the south-eastern part of the karstic Livanjsko Polje Valley. This aquatic ecosystem is distinguished by a very rich ichthyofauna and the presence of four endemic fish species. Numerous studies focus on ichthyological researches of Buško Blato Reservoir. Its ichthyofauna has been exposed to changes in ecological factors, which in turn reflected on the structure and composition of fish populations. The main objective of this paper was to assess the current state and predict future trends in the ichthyofauna structure and dynamics based on the field data and comprehensive analyses of literature data. The results of the research indicated the presence of 11 fish species from four families, which is the largest number of fish species ever recorded in this ecosystem. Sander lucioperca, Lepomis gibbosus, Pseudorasbora parva and Tinca tinca were recorded for the first time in this ecosystem, while some previously recorded species were not found. The results of the analyses clearly indicate the presence of natural interspecific competition and significant level of threats to the endemic fish species caused by human activities