Dosage du tritium et du carbone 14 dans les tissus animaux par scintillation liquide

peer reviewed1. 1. Mouse organs are digested in hyamine hydroxide methanolic solution and the digest is diluted with dioxane before the addition of a toluene scintillating solution. 2. 2. The vials ready for counting contain up to 155 mg of fresh tissue in a volume of 16 ml. 3. 3. Without digest, with the Packard Tri-Carb spectrometer, the yields of counting of the final scintillating mixture are 50% for 14C and 17.5% for 3H. With 100 mg of stomach, intestine, brain or muscle, the yields are 40% for 14C and 5% for 3H. They are half these values with 100 mg of liver, lung or heart. 4. 4. The chemiluminescence with the Tri-Carb spectrometer is negligible with 14C, but a waiting period of 48 h is necessary for counting 3H. During this time, the vials can stay at room temperature without particular screening from the light. © 1963