Dynamic Light Scattering Analysis on RNA Associated to Proteins

International audienceDynamic Light Scattering represents an accurate, robust, and reliable technique to analyze molecules size in solution, and monitor their interactions in real time. Here, we describe how to analyze by DLS an RNA-protein interaction. In our frame, we studied complexes formed between RNA fragments derived from the genome of HIV-1 in association with the viral precursor Pr55 Gag. These interactions are crucial for the specific selection of the viral genomic RNA (gRNA) from the bulk of the viral spliced and cellular RNAs. This chapter displays how DLS allows to characterize the interactions that regulate the early steps of viral assembly

Anuric, obstructive uropathy in the absence of obvious radiological evidence of obstruction

Journal compilation © 2004 Royal Australasian College of Surgeons Copyright © 2004 John Wiley & Sons, Inc. All Rights Reserved.Wing-Seng Leong , Henry Sells and Kim L. Morett