Validation studies of gyrokinetic ITG and TEM turbulence simulations in a JT-60U tokamak using multiple flux matching

Quantitative validation studies of flux-tube gyrokinetic Vlasov simulations on ion and electronheat transport are carried out for the JT-60U tokamak experiment. The ion temperaturegradient (ITG) and/or trapped electron modes (TEM) driven turbulent transport and zonalflow generations are investigated for an L-mode plasma in the local turbulence limit with asufficiently small normalized ion thermal gyroradius and weak mean radial electric fields.Nonlinear turbulence simulations by the GKV code successfully reproduce radial profilesof the ion and electron energy fluxes in the core region. The numerical results show that theTEM-driven zonal flow generation in the outer region is more significant than that in the coreregion with ITG- and ITG–TEM-dominated turbulence, leading to moderate transport shortfallof the ion energy flux. Error levels in the prediction of the ion and electron temperaturegradient profiles in the core region are estimated as less than ±30%, based on a multiple fluxmatching technique, where the simulated ion and electron energy fluxes are simultaneouslymatched to the experimental values

Intraoperative diagnosis of lymph node metastasis during segmentectomy for non-small cell lung cancer by rapid immunohistochemistry using noncontact alternating current electric field mixing

Background: Although lobectomy is considered the standard surgery for any non-small cell lung cancer (NSCLC), recent evidence indicates that for early NSCLCs segmentectomy may be equally effective. For segmentectomy to be oncologically safe, however, adequate intraoperative lymph node staging is essential. The aim of this study was to compare the results of a new rapid-IHC system to the HE analysis for intraoperative nodal diagnosis in lung cancer patients considered for segmentectomy. Methods: This retrospective study analyzed the pathological reports from NSCLC resections over a six-year period between 2014 and 2020. Using a new device for rapid-IHC, we applied a high-voltage, low-frequency alternating current (AC) field, which mixes the antipancytokeratin antibody as the voltage is switched on/off. Rapid-IHC can provide a nodal diagnosis within 20 minutes. Results: Frozen sections from 106 resected lymph nodes from 70 patients were intraoperatively evaluated for metastasis. Of those, five nodes were deemed positive based on both HE staining and rapid-IHC. In addition, rapid-IHC alone detected isolated tumor cells in one hilar lymph node. Three cStage IA patients with nodal metastasis detected with HE staining and rapid-IHC received complete lobectomies. Five-year relapse-free survival and overall survival among patients receiving segmentectomy with rapid-IHC were 88.77% and 88.79%, respectively. Conclusions: Rapid-IHC driven by AC mixing is simple, highly accurate, and preserves nodal tissue for subsequent tests. This system can be used effectively for intraoperative nodal diagnosis. Rapid immunohistochemistry based on alternating-current field mixing (completed within 20 minutes) is simple and highly accurate. This system will assist clinicians when making intraoperative diagnoses of lymph node metastasis and deciding upon the appropriate surgical procedure in segmentectomy for lung cancer

Scaling study for positive magnetic shear ELMy H-mode plasmas

Scaling of the density peaking for positive magnetic shear ELMy H-mode plasmas in JT-60U has been developed. Although the density peaking generally depends on collisionality, a variation of the density peaking factor for the same collisionality exists, and the variation is different between plasmas with co-directed and ctr-directed toroidal rotation(co-V T plasmas and ctr-V T plasmas). The variation of the co-V T plasmas can be explained by particle source profile. As a result of scaling, the peaking factor of the co-V T plasmas depends on the particle source rate profile from neutral beams (NBs) as much as collisionality. The dataset of the ctr-V T plasmas has a larger variation of the peaking factor compared to that of the co-V T plasmas. The larger variation stems from that the peaking factor of the ctr-V T plasmas also depends on the normalized ion temperature gradient at the edge region. As a result of scaling, the normalized ion temperature gradient influences the peaking factor as much as or a little larger than collisionality. The parameter regime of the ctr-V T plasmas ranges from the trapped electron mode (TEM) to the ion temperature gradient mode (ITG mode). The plasmas with the positive correlation between the normalized density gradient and the normalized ion temperature gradient are dominated by the TEM. On the other hand, the plasmas with the negative correlation are dominated by the ITG mode

Prophylaxis of Antifungal Drugs against Systemic Fungemia Induced by Oral Candidiasis in Mice

Oral mucositis is highly prevalent among the elderly, for whom oral care is often difficult. Oral mucositis, such as candidiasis, can induce systemic fungemia. Antifungal prophylaxis may be useful in such cases to prevent systemic fungemia; however, studies on this are limited. The objective of this study was to demonstrate the effectiveness of antifungal prophylaxis to prevent systemic Candida dissemination compared to oral care using a mice model. Oral candidiasis was induced using chemotherapy and inoculation with C. albicans in 8-week-old male mice. Group A was given oral care, Group B was orally administered an antifungal drug, Group C was intravenously administered an antifungal drug, and Group D was used as the negative control group. Macroscopic features of the tongue surface, colony forming units (CFU) on the tongue, and blood culture for C. albicans were evaluated. CFU was significantly higher in Group A than in Groups B and C. The oral care group, but not the groups administered antifungal agents, showed significantly higher positive numbers of animals with C. albicans in the blood as compared to the control group, indicating the effectiveness of antifungal prophylaxis over oral care. Antifungal prophylaxis may be an option for the prevention of systemic fungemia in individuals with difficulty in oral care

Prophylaxis of Antifungal Drugs against Systemic Fungemia induced by Oral Candidiasis in Mice

Oral mucositis is highly prevalent among the elderly, for whom oral care is often difficult. Oral mucositis, such as candidiasis, can induce systemic fungemia. Antifungal prophylaxis may be useful in such cases to prevent systemic fungemia; however, studies on this are limited. The objective of this study was to demonstrate the effectiveness of antifungal prophylaxis to prevent systemic Candida dissemination compared to oral care using a mice model. Oral candidiasis was induced using chemotherapy and inoculation with C. albicans in 8-week-old male mice. Group A was given oral care, Group B was orally administered an antifungal drug, Group C was intravenously administered an antifungal drug, and Group D was used as the negative control group. Macroscopic features of the tongue surface, colony forming units (CFU) on the tongue, and blood culture for C. albicans were evaluated. CFU was significantly higher in Group A than in Groups B and C. The oral care group, but not the groups administered antifungal agents, showed significantly higher positive numbers of animals with C. albicans in the blood as compared to the control group, indicating the effectiveness of antifungal prophylaxis over oral care. Antifungal prophylaxis may be an option for the prevention of systemic fungemia in individuals with difficulty in oral care

Reg Gene Expression in Periosteum after Fracture and Its In Vitro Induction Triggered by IL-6

The periosteum is a thin membrane that surrounds the outer surface of bones and participates in fracture healing. However, the molecular signals that trigger/initiate the periosteal reaction are not well established. We fractured the rat femoral bone at the diaphysis and fixed it with an intramedullary inserted wire, and the expression of regenerating gene (Reg) I, which encodes a tissue regeneration/growth factor, was analyzed. Neither bone/marrow nor muscle showed Reg I gene expression before or after the fracture. By contrast, the periosteum showed an elevated expression after the fracture, thereby confirming the localization of Reg I expression exclusively in the periosteum around the fractured areas. Expression of the Reg family increased after the fracture, followed by a decrease to basal levels by six weeks, when the fracture had almost healed. In vitro cultures of periosteal cells showed no Reg I expression, but the addition of IL-6 significantly induced Reg I gene expression. The addition of IL-6 also increased the cell number and reduced pro-apoptotic gene expression of Bim. The increased cell proliferation and reduction in Bim gene expression were abolished by transfection with Reg I siRNA, indicating that these IL-6-dependent effects require the Reg I gene expression. These results indicate the involvement of the IL-6/Reg pathway in the osteogenic response of the periosteum, which leads to fracture repair

Association of Cadaveric Factors with the Degree and Region of Discoloration on Pink Teeth: An Approach to Serial Cases

The pink teeth phenomenon has been observed in cases of hanging and drowning, but the relationship of this phenomenon with cause of death has not been positively affirmed. To date, teeth with a pinkish tone have been simply regarded as pink teeth. However, we speculated that classification and evaluation of the degree and region of discoloration with reference to how color is classified in prosthodontic dentistry may produce novel findings regarding the pink teeth phenomenon. Therefore, we classified the degree and region of discoloration on teeth into three grades and attempted to evaluate the relationship of grade with various cadaveric factors in 68 cases of pink teeth. The degree and region scores of discoloration did not show significant differences in terms of sex, age, cause of death, and place of discovery, but the degree of discoloration in terms of time after death showed a significant increase at 6 months (p p p < 0.01). Thus, it was clarified that time after death was most strongly related to the pink teeth phenomenon, suggesting cadaveric decomposition may affect the occurrence of pink teeth. Further investigation in cases of early postmortem changes would be required to determine whether pink teeth in putrefactive cadavers have utility in medico–legal assessments