Novel optoelectronic platform for label-free biosensing of influenza detection based on interferometric transducers

The main goal of this project is to present an Optical Label-free Point-ofCare Device based on a novel read-out methodology that enhances significantly the biosensing response in terms of sensitivity and LoD, using a simple, fast and reliable interrogation process. The performance of this PoC device is verified by carrying out the calibration curve for the indirect immunoassay of Influenza Virus and comparing it with high-resolution spectrometry using the same Fabry-Perot interferometers as biosensors

A New Device Based on Interferometric Optical Detection Method for Label-Free Screening of C-Reactive Protein

In previous work, the performance of a compact and cost-effective point-of-care (PoC) device based on the increase relative optical power (IROP) methodology is reported and it is determined the enhancement in comparison with standard high-resolution spectrometry in terms of limit of detection. This paper describes a new label-free IROP-based biomedical device capable of working with low concentration of reagents and low sample volume per measurement in order to be used for screening different steps necessary in immunoassay optimization. This new approach significantly improves the sensing performance in terms of read-out signal (AIROP) per nanometer of biofilm in comparison with our previous work. This improvement is achieved due to the implementation of a laser as light source of the optical read-out system and the redesign of Fabry-Perot transducers by optimizing their reflectivity response and reducing their sensing area. For demonstrating the screening capability of this new PoC device in several immunoassays steps and methodologies, a C-reactive protein detection assay was carried out as a potential application and assay model. It is remarkable that only 10 μL of sample was used per measurement. This labelfree IROP-based device complies an easy-to-use and cost-effective tool for immunoassays optimization in terms of performance, reagents cost, and measuring time

Developing optical label-free Point-of-Care biosensing devices

The aim of this work is to develop a compact point-of-care device as a proof of concept of a novel read-out methodology than enhances the biosensing response by improving significantly the LoD and makes the interrogation process simple and more reliable. The measured signal is called Increase Relative Optical Power (IROP) and is a function of the optical power or irradiance for a certain optical interrogation band of two interferometers (reference and signal). The functionality of this device is validated by performing standard immunoassays and comparing these results with high resolution spectrometry

Biochemical assay development for optimization of immunological system inhibitors

The aim of this work was to adapt BioD Point of Care technology in order to evaluate immunologic system inhibitors through a chemical assay that is able to identify the inhibition ability of new drugs for the treatment of autoimmune disease

Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis

A novel compact optical biochip based on a thin layer-sensing surface of nitrocellulose is used for in-situ label-free detection of metalloproteinase (MMP9) related to dry eye disease. In this article, a new integrated chip with different interferometric transducers layout with an optimal sensing surface is reported for the first time. We demonstrate that specific antibodies can be immobilized onto these transducers with a very low volume of sample and with good orientation. Many sensing transducers constitute the presented biochip in order to yield statistical data and stability in the acquired measurements. As a result, we report the recognition curve for pure recombinant MMP9, tests of model tears with MMP9, and real tear performance from patients, with a promising limit of detection

Resonant nanopillars as label-free optical biosensors

In recent works, it has been demonstrated the suitability of using resonant nanopillars (R-NPs) as biochemical. In this work, it has been shown the capability of the R-NPs to behave as label-free multiplexed biological sensors. Each R-NP is formed by silicon oxide (SiO2) and silicon nitride (Si3N4) Bragg reflectors and a central cavity of SiO2, and they are grouped into eight arrays called BICELLs, which are distributed on a single chip of quartz substrate for multiplexing measurements. For the biological sensing assessment, it was developed an immunoassay on the eight single BICELLs. The biofunctionalization process was performed by a silanization protocol based on 3-aminopropyltrymethoxysilane (APTMS) and glutaradheyde (GA) as a linker between APTMS and the IgG which acted as bioreceptor for the anti-IgG recognition. In this work, there were compared two forms of immobilization: on one hand by incubating the R-NPs under static drop of 50 ?g/mL and on the second hand by introducing the sensing chip in a flow cell with a continuous flow of the same concentration of IgG. The eight arrays of R-NPs or BICELLs were independently optically interrogated by a bundle of fiber connected to a spectrometer. The multiplexing analysis showed reproducibility among the BICELLs, suggesting the potentially of using R-NPs for multiplexed biosensors. Performance in the immobilization process apparently does not have a signification effect. However, the election of one method or another should be a commitment between time and resources

Novel optoelectronic platform for label-free biosensing of influenza detection based on interferometric transducers

The main goal of this project is to present an Optical Label-free Point-ofCare Device based on a novel read-out methodology that enhances significantly the biosensing response in terms of sensitivity and LoD, using a simple, fast and reliable interrogation process. The performance of this PoC device is verified by carrying out the calibration curve for the indirect immunoassay of Influenza Virus and comparing it with high-resolution spectrometry using the same Fabry-Perot interferometers as biosensors