Clinical And Radiographic Assessment of Xenogenic Bone Graft With Or Without Hyaluronic Acid For Post Extraction Socket Preservation (Randomized Clinical Trial)

hyaluronic acid shows osteogenic potentials which considered important property during bone regeneration. So, we aim to assess the efficiency of hyaluronic acid with xenograft mixture in post extraction alveolar sockets to decelerate resorption of dental alveolar ridge

Effect of Different Packing Types on Storage and Quality of Saidy Dates

This study was conducted during two successive seasons 2020/2021 and 2021/2022 to study the effect of some packing types on the storability and quality of saidy dates (Phoenix dactylifera L.) fruits during the storage period. The post-harvest treatments were packed with perforated or non-perforated polyethylene layers and aluminum foil. The fruits were stored at cold condition 5±1°C with 85-90% RH. Samples of each treatment were randomly taken every two months for 8 months. The results showed that fruit weight loss %, fruit damage %, total soluble solids % and sugar contents were significantly increased with prolonging the storage period. On other hand, the advanced storage period induced a gradual decrease of fruit weight, flesh% and fruit dimensions. All packing with non-perforated either polyethylene or aluminum foil caused a decrease in fruit weight loss and fruit damage percentage as well as fruit weight, flesh% and fruit dimensions compared to use other packing types .Using non-perforated polyethylene result in the least fruit weight loss and fruit damage percentage compared to use other packing types. Using non-perforated polyethylene packing improved the fruit quality during the storage period compared to use perforated polyethylene or either perforated or non-perforated aluminum foil. It could be concluded that using Packing with non-perforated polyethylene maintained fruit freshness without negative effects of fruit quality parameters and seems to be the proper and an ideal packing types to prolong cold storage of saidy dates without great reduction in fruit quality

The impact of co-infections on fish: a review

International audienceAbstractCo-infections are very common in nature and occur when hosts are infected by two or more different pathogens either by simultaneous or secondary infections so that two or more infectious agents are active together in the same host. Co-infections have a fundamental effect and can alter the course and the severity of different fish diseases. However, co-infection effect has still received limited scrutiny in aquatic animals like fish and available data on this subject is still scarce. The susceptibility of fish to different pathogens could be changed during mixed infections causing the appearance of sudden fish outbreaks. In this review, we focus on the synergistic and antagonistic interactions occurring during co-infections by homologous or heterologous pathogens. We present a concise summary about the present knowledge regarding co-infections in fish. More research is needed to better understand the immune response of fish during mixed infections as these could have an important impact on the development of new strategies for disease control programs and vaccination in fish

Microbes At A Non-Point Source Sub-Tropical Recreational Marine Beach And Their Implications On Human Health And Beach Monitoring Practices

Swimming in ocean water, including beaches not impacted by known point sources of pollution, continues to pose a rising health concern. Studies which evaluate the relationship between indicator microbes, pathogens, and human health at recreational beaches are rare, especially at non-point beaches, although such studies are necessary for establishing criteria which would protect public health while minimizing economic burdens. The objective of this dissertation was to better understand the non-point source beach system, and hence determine possible directions that should be taken from a beach regulatory perspective in order to protect bather health and minimize economic burdens. This objective was accomplished through two studies. The first study included an initial evaluation of the presence of and associations among indicator microbes, pathogens and environmental conditions at a subtropical, recreational marine beach in South Florida (USA) impacted by non-point sources of pollution. Twelve water and eight sand samples were collected during four sampling events conducted at high or low tide and during elevated or reduced solar insolation conditions. Analyses included fecal indicator bacteria (FIB) (fecal coliform, Escherichia coli, enterococci, Clostridium perfringens), human-associated microbial source tracking (MST) markers (human polyomaviruses [HPyVs] and Enterococcus faecium esp gene), and pathogens (Vibrio vulnificus, Staphylococcus aureus, enterovirus, norovirus, hepatitis A virus, Cryptosporidium spp. and Giardia spp.). The objective of the second study was to capture a series of snapshots of the study beach with respect to pathogens and indicator microbe levels, environmental conditions, and cumulative human health effects. Two sets of daily composite samples were collected during the BEACHES epidemiology study conducted in Miami (FL) at the same non-point source subtropical marine beach. These samples were analyzed for several pathogens, microbial source tracking markers, indicator microbes, and environmental parameters similar to those in the first study. Enterococci concentrations in water and sand assessed by qPCR were greater than membrane filtration measurements in both studies. FIB concentrations in water were below recreational water quality standards for three of four sampling events; pathogens and MST markers were also generally undetectable during the first study. FIB levels exceeded regulatory guidelines during one event, accompanied by the detection of HPyVs and pathogens, including the autochthonous bacterium Vibrio vulnificus in both sand and water, the allochthonous protozoan Giardia spp. in water, and Cryptosporidium spp. in sand samples. These elevated microbial levels were detected at high tide, low solar insolation conditions, and after elevated 2 day antecedent rainfall. Analyses of the second study demonstrated that rainfall and tide were also significant factors in determining the presence of both indicator microbes and pathogens. Additional potential associations between environmental factors, microbes and human health effects were also identified. However, more analyses should be conducted as the limited number of samples renders it difficult to make conclusive statements about the relationship between these different factors. The results of this research demonstrate the complexity of beach systems characterized by non-point sources, and how more novel and comprehensive approaches will be needed to assess beach water quality for the purpose of protecting bather health. A recommended approach The comprehensive tool box with approval process approach to address the beach complexity is presented and applied to the study beach

Complications of Port A Cath implantation: A single institution experience

Objectives: To determine the complications associated with Port A Cath insertion in cancer patients. Methods: The records 250 patients, who received a subcutaneous port catheter between 2009 and 2013, were analyzed retrospectively with regard to implantation complications and complications in the course of Port A Cath use. Results: The average duration over which the Port A Cath remained in place was 22 months. Postoperative complications occurred in 29 patients (11.6%); of these, 4 (1.6%) were perioperative and 25 (10%) were long-term complications. Perioperative complications were in the form of inadvertent arterial rupture. Long-term complications included the following: infection in 10 patients (4%), mechanical failure in 5 patients (2%), thrombosis in 4 patients (1.6%), suture disruption in 3 patients (1.2%), extravasation in 2 patients (0.8%), and catheter migration in one patient (0.4%). Conclusion: Port A Cath implantation is associated with some risk of serious complications. Care of the catheter and the patient should be maintained to decrease the risk of complications

The impact of co-infections on fish:a review

International audienceAbstractCo-infections are very common in nature and occur when hosts are infected by two or more different pathogens either by simultaneous or secondary infections so that two or more infectious agents are active together in the same host. Co-infections have a fundamental effect and can alter the course and the severity of different fish diseases. However, co-infection effect has still received limited scrutiny in aquatic animals like fish and available data on this subject is still scarce. The susceptibility of fish to different pathogens could be changed during mixed infections causing the appearance of sudden fish outbreaks. In this review, we focus on the synergistic and antagonistic interactions occurring during co-infections by homologous or heterologous pathogens. We present a concise summary about the present knowledge regarding co-infections in fish. More research is needed to better understand the immune response of fish during mixed infections as these could have an important impact on the development of new strategies for disease control programs and vaccination in fish

The impact of Tetracapsuloides bryosalmonae and Myxobolus cerebralis co-infections on pathology in rainbow trout

Abstract Background Myxozoan parasites pose emerging health issues for wild and farmed salmonid fish. Rainbow trout (Oncorhynchus mykiss) is a particularly susceptible species to Tetracapsuloides bryosalmonae (Malacosporea), the etiological agent of Proliferative Kidney Disease (PKD), and to Myxobolus cerebralis (Myxosporea), the etiological agent of Whirling Disease (WD). The objective of this study was to investigate the impact of myxozoan co-infections on the pathogenesis of PKD and WD in the rainbow trout. Methods Two groups of rainbow trout (96 fish each) were primarily infected with T. bryosalmonae and triactinomyxons of M. cerebralis; after 30 days half of the fish in each group were co-infected with these parasites vice versa and remaining half was continued as single infection. Mortalities and clinical signs were recorded at different time points. Histopathology and immunohistochemistry were performed to assess the extent of each infection and estimate the parasite burden between groups. Results Fish firstly infected with M. cerebralis and co-infected with T. bryosalmonae exhibited exacerbated pathological changes of both parasitic diseases and elicited a higher mortality rate. A higher kidney swelling index (grade 4) appeared together with more severe cartilage destruction and displacement, when compared to the pathological changes in fish upon single infections with T. bryosalmonae or M. cerebralis. Conversely, fish firstly infected with T. bryosalmonae and co-infected with M. cerebralis also exhibited typical pathological changes of both parasitic diseases, but with a lower mortality rate, similar as caused by the single T. bryosalmonae or M. cerebralis infection. WD clinical signs were milder, without skeletal deformities, while kidney swelling index was similar to single infection with T. bryosalmonae (grade 2 to 3). Conclusions In this study, a co-infection with myxozoan parasites was for the first time successfully achieved in the laboratory under controlled conditions. The impact of co-infections in concurrent myxozoan infections mainly depends on the primary pathogen infecting the host, which could alter the outcomes of the secondary pathogen infection. The primary M. cerebralis infection followed by T. bryosalmonae had a much more serious impact and elicited a synergistic interaction. Contrasting results were instead seen in rainbow trout primarily infected with T. bryosalmonae and then co-infected with M. cerebralis

Differential modulation of host immune genes in the kidney and cranium of the rainbow trout (Oncorhynchus mykiss) in response to Tetracapsuloides bryosalmonae and Myxobolus cerebralis co-infections

Abstract Background Most of the studies on fish diseases focus on single infections, although in nature co-infections occur more often. The two freshwater myxozoan parasites of salmonids, having high economic and ecologic relevance are Tetracapsuloides bryosalmonae (Malacosporea), the etiological agent of proliferative kidney disease, and Myxobolus cerebralis (Myxosporea), the etiological agent of whirling disease. The present study aims to investigate immune modulation in rainbow trouts (Oncorhynchus mykiss) during single and co-infections by these parasites. Methods Fish were initially infected with T. bryosalmonae (one group) and M. cerebralis (another group) separately. At 30 days post-exposure (dpe), both the single species infected groups were co-infected, respectively, with the other parasite. Posterior kidney and cartilage cranium samples were collected at 30, 60, 90 and 120 dpe and RT-qPCR was performed on them to assess the transcription of suppressors of cytokine signaling (SOCS) -1 and -3, Janus kinase-1 (JAK-1) and signal transducer and activator of transcription-3 (STAT-3) genes. Results Kidney samples from the T. bryosalmonae-infected group showed upregulation of all immune genes tested between 60–120 dpe. Crania from the single M. cerebralis-infected group and the M. cerebralis and T. bryosalmonae co-infected group exhibited upregulation of SOCS-1 and JAK-1 between 60–120 dpe and SOCS-3 at 120 dpe. However, only in the single M. cerebralis-infected group, was a statistically significant expression of STAT-3 observed at 30 and 60 dpe. Conclusions The results of this study indicate that both T. bryosalmonae and M. cerebralis induce overexpression of SOCS-1 and SOCS-3 genes and modulate the host immune response during the development of parasite to cause immunosuppression