Chemical properties and shelf life of velvet apple germplasm

The study was undertaken to evaluate bio-chemical properties, shelf-life determination and expansion of ripe fruits of four velvet apple germplasm. The selected germplasm were local red variety, local yellow variety, PSTU Bilati gab-1 and PSTU Bilati gab-2. Ripe fruits were collected from Germplasm Center, Patuakhali Science and Technology University (PSTU) and different locations of Patuakhali district of Bangladesh. Results expressed that maximum TSS (13.93%), vitamin-C (3.68 mg/100g), non-reducing sugar (5.15%) and total sugar (7.95%) were recorded from PSTU Bilati gab-2, the highest TA (1.30%) was found in PSTU Bilati gab-1 but highest pulp pH (7.50) and TSS to TA ratio (11.11) were recorded from local yellow variety whereas highest reducing sugar (3.17%) was recorded from local red variety. The shelf life of ripe fruit varied from 3.25 – 4.75 days where the longest shelf life was obtained from PSTU Bilati gab-2 and the shortest shelf life was recorded in local red variety. But the storability was extended more than 27 days when stored in “Refrigerator” followed by “Non-perforated polybag (NPP)” (10 days), perforated polybag (8 days) and paper bag (6 days). The firmness of fruits were decreased gradually with days but the reduction was minimized when stored in different storage condition where more firmness hold in refrigerator treatment followed by NPP. Similarly, the weight loss was controlled in refrigerator followed by NPP treatment. The results of the experiments revealed that PSTU Bilati gab-2 provides superior chemical properties among the germplasm and ‘Refrigerator’ treatment exhibited best performance among other

Isolation, screening and molecular identification of antagonistic bacteria against Colletotrichum gloeosporioides in mango

The study was conducted at the Postharvest and plant Biotechnology laboratory, Department of Horticulture, Patuakhali Science and Technology University, Patuakhali, Bangladesh during the period from January to July 2017 to isolate, screening and molecular identification of antagonistic bacteria against anthracnose of mango. All treatments were arranged in a completely randomized design (CRD) with replications and repeated twice. Epiphytic bacteria, isolated from leaf and fruit surfaces of mango, were tested as biocontrol agent against anthracnose disease caused by Colletotrichum gloeosporioides wherein 20 strains were confirmed as antagonistic. Molecular characterization of the three potential strains of bacteria were done by the amplification of 16S rDNA gene following the extraction of genomic DNA, polymerase chain reaction (PCR) amplification, gel electrophoresis and gel documentation. The PCR amplified products and the genomic DNA samples were sent to the Macrogen Company through Sunchon National University, Seoul, South Korea for molecular identification by sequence analysis. Among the 20 antagonistic bacteria screened in vitro by dual and concomitant tests, two isolates, namely GB6 (PSTU-Hort-8), and GB19 (PSTU-Hort-14) were recognized as antagonistics to the test fungus. Using the molecular identification systems, isolated bacterial strains PSTU-Hort-8 was identified as B. subtilis with National Center for Biotechnology Information (NCBI) accession numbers MW659188; on the other hand, strain PSTU-Hort-14 was identified as Stenotrophomonas rhizophila with NCBI accession number MW659190

第1回AFC国際シンポジウム報告と講演要旨

Article信州大学農学部AFC報告 2: 95-102(2004)departmental bulletin pape