75 research outputs found
Influence of cutting height on biomass yield and quality of miscanthus genotypes
Abstract Commercially achieved biomass yields are often lower than those obtained in scientific plot trials and estimated by crop models. This phenomenon is commonly referred to as the âcommercial yield gapâ. It needs to be understood and managed to achieve the yield expectations that underpin business models. Cutting height at harvest is one of the key factors determining biomass yield and quality. This study quantifies the impacts of cutting heights of diverse genotypes with different morphologies and in years with contrasting weather conditions before and during harvest. Harvests were made in March 2015 and March 2018 of six diverse miscanthus genotypes planted as part of the âOPTIMISC projectâ in 2013 near Stuttgart, Germany. Biomass yield, dry matter content and nutrient concentrations were analysed in four 10 cm fractions working upwards from the ground level and a fifth fraction with the shoot biomass higher than 40 cm. As stems are slightly tapered (i.e. diameter decreases slightly with increasing cutting height), it was hypothesized that low cutting may lead to yield gains, but that these may be associated with lower quality biomass with higher moisture and higher nutrient offtakes. We calculated average yield losses of 270 kg haâ1 (0.83%) with each 1 cm increase in cutting height up to 40 cm. Although whole shoot mineral concentrations were significantly influenced by both genotype and year interactions, total nitrogen (1.89 mg gâ1), phosphorus (0.51 mg gâ1), potassium (3.72 mg gâ1) and calcium (0.89 mg gâ1) concentrations did not differ significantly from the concentrations in the lower basal sections. Overall, cutting height had a limited influence on nutrient and moisture content. Therefore, we recommend that cutting is performed as low as is practically possible with the available machinery and local ground surface conditions to maximize biomass yield
The Use of Early Spirometric Changes and IGF-1 Levels for the Detection of Patients at Risk for BOS
Routine Prophylaxis of Pneumocystis Jirovecii Pneumonia in Recipients of Autologous Hematopoietic Stem Cell Transplantation
Early impacts of marginal land-use transition to Miscanthus on soil quality and soil carbon storage across Europe
The authors acknowledged INA laboratories for performing soil chemical analysis, in particular SlijepÄeviÄ Boris and Rukavina Slavica.Peer reviewe
Site impacts nutrient translocation efficiency in intraspecies and interspecies miscanthus hybrids on marginal lands
Miscanthus, a C4 perennial rhizomatous grass, is capable of growing in varied climates and soil types in Europe, including on marginal lands. It can produce high yields with low nutrient inputs when harvested after complete senescence. Senescence induction and rate depend on complex genetic, environmental, and management interactions. To explore these interactions, we analysed four miscanthus hybrids (two novel seed-based hybrids, GRC 3 [Miscanthus sinensis Ă sinensis] and GRC 14 [M. sacchariflorus Ă sinensis]; GRC 15, a novel M. sacchariflorus Ă sinensis clone; and GRC 9, a standard Miscanthus Ă giganteus clone) in Italy, Croatia, Germany and the UK. Over all trial locations and hybrids, the average aboveground biomass of the 3-year-old stands in August 2020 was 15 t DM haâ1 with nutrient contents of 7.6 mg N gâ1 and 14.6 mg K gâ1. As expected, delaying the harvest until spring reduced overall yield and nutrient contents (12 t DM haâ1, 3.3 mg N gâ1, and 5.5 mg K gâ1). At lower latitudes, the late-ripening M. sacchariflorus Ă sinensis GRC 14 and GRC 15 combined high yields with low nutrient contents. At the most elevated latitude location (UK), the early-ripening M. sinensis Ă sinensis combined high biomass yields with low nutrient offtakes. The clonal Miscanthus Ă giganteus with intermediate flowering and senescence attained similar low nutrient contents by spring harvest at all four locations. Seasonal changes in yield and nutrient levels analysed in this study provide: (1) a first step towards recommending hybrids for specific locations and end uses in Europe; (2) crucial data for determination of harvest time and practical steps in the valorization of biomass; and (3) key sustainability data for life cycle assessments. Identification of trade-offs resulting from genetic Ă environment Ă management interactions is critical for increasing sustainable biomass supply from miscanthus grown on marginal lands
Etanercept plus Topical Corticosteroids as Initial Therapy for Grade One Acute Graft-Versus-Host Disease after Allogeneic Hematopoietic Cell Transplantation
AbstractClinical diagnosis of grade 1 acute graft-versus-host disease (GVHD) marks the beginning of a potentially progressive and fatal course of GVHD after hematopoietic stem cell transplantation (HSCT). However, interventional studies to treat early GVHD are lacking. We conducted a single-arm prospective phase II trial to test the hypothesis that treatment of newly diagnosed grade 1 acute GVHD with etanercept and topical corticosteroids would reduce progression to grade 2 to 4 within 28 days. Study patients (n = 34) had a median age of 51 years (range, 10 to 67 years) and had undergone unrelated (n = 22) or related (n = 12) donor HSCT. Study patients were treated with etanercept (.4 mg/kg, maximum 25 mg/dose) twice weekly for 4 to 8 weeks. Ten of 34 patients (29%) progressed to grade 2 to 4 acute GVHD within 28 days. The cumulative incidence of grade 2 to 4 and grade 3 to 4 acute GVHD at 1 year was 41% and 3%, respectively. Nonrelapse mortality was 19% and overall survival was 63% at 2 years. Among a contemporaneous control cohort of patients who were diagnosed with grade 1 acute GVHD and treated with topical corticosteroids but not etanercept during the study period, 12 of 28 patients (43%) progressed to grade 2 to 4 GVHD within 28 days, with a 1-year incidence of grade 2 to 4 GVHD and grade 3 to 4 GVHD of 61% (41% versus 61%, P = .08) and 18% (3% versus 18%, P = .05), respectively. Patients treated with etanercept also experienced less increase in GVHD plasma biomarkers suppression of tumorigenicity 2 (P = .06) and regenerating islet-derived 3-alpha (P = .01) 28 days after grade 1 acute GVHD diagnosis compared with contemporaneous control patients. This study was terminated early because of poor accrual. Future prospective studies are needed to identify patients with grade 1 acute GVHD at risk of swift progression to more severe GVHD and to establish consensus for the treatment of grade 1 acute GVHD. This trial is registered with ClinicalTrials.gov, number NCT00726375
Spring emergence and canopy development strategies in miscanthus hybrids in Mediterranean, continental and maritime European climates
Spring emergence and canopy development strategies in miscanthus hybrids in Mediterranean, continental and maritime European climates
Abstract Due to its versatility and storability, biomass is an important resource for renewable materials and energy. Miscanthus hybrids combine high yield potential, low input demand, tolerance of certain marginal land types and several ecosystem benefits. To date, miscanthus breeding has focussed on increasing yield potential by maximising radiation interception through: (1) selection for early emergence, (2) increasing the growth rate to reach canopy closure as fast as possible, and (3) delayed flowering and senescence. The objective of this study is to compare early season reâgrowth in miscanthus hybrids cultivated across Europe. Determination of differences in early canopy development on endâofâyear yield traits is required to provide information for breeding decisions to improve future crop performance. For this purpose, a trial was planted with four miscanthus hybrids (two novel seedâbased hybrids M. sinensisâĂâsinensis [M sinâĂâsin] and M. sacchariflorusâĂâsinensis [M sacâĂâsin], a novel rhizomeâbased M sacâĂâsin and a standard Miscanthus Ă giganteus [M Ă g] clone) in the UK, Germany, Croatia and Italy, and was monitored in the third and fourth growing season. We determined differences between the hybrids in base temperature, frost sensitivity and emergence strategy. MâĂâg and M sac Ă sin mainly emerged from belowground plant organs, producing fewer but thicker shoots at the beginning of the growing season but these shoots were susceptible to air frosts (determined by recording 0°C 2 m above ground surface). By contrast, M sinâĂâsin emerged 10âdays earlier, avoiding damage by late spring frosts and producing a high number of thinner shoots from aboveground shoots. Therefore, we recommend cultivating M sacâĂâsin at locations with low risk and M sinâĂâsin at locations with higher risk of late spring frosts. Selecting miscanthus hybrids that produce shoots throughout the vegetation period is an effective strategy to limit the risk of late frost damage and avoid reduction in yield from a shortened growing season
Yield performance of 14 novel inter- and intra-species Miscanthus hybrids across Europe
Funding information Bio-Based Industries Joint Undertaking, Grant/Award Number: 745012 ACKNOWLEDGEMENTS For additional information and data collection many thanks to Oberer Lindenhof field station staff (OLI), Unifarm workers (SCH), experimental station Ć aĆĄinovec technical stuff (ZAG). The authors are grateful for the support of the staff at the research stations at PAC. With particular thanks at the Trawsgoed site (TWS) to Robin Warren, Chris Glover, and the late Kevin Roderick. Thanks also to Michael Squance for use of and assistance with the Physisâą data management platform. FUNDING INFORMATION The GRACE project has received funding from the Bio-based Industries Joint Undertaking (JU) under the European Union's Horizon 2020 research and innovation programme under grant agreement no. 745012. The JU receives support from the European Union's Horizon 2020 research and innovation programme and the Bio-based Industries Consortium.Peer reviewedPublisher PD
Single-cell transcriptomics reveals involution mimicry during the specification of the basal breast cancer subtype
Basal breast cancer is associated with younger age, early relapse, and a high mortality rate. Here, we use unbiased droplet-based single-cell RNA sequencing (RNA-seq) to elucidate the cellular basis of tumor progression during the specification of the basal breast cancer subtype from the luminal progenitor population in the MMTV-PyMT (mouse mammary tumor virus-polyoma middle tumor-antigen) mammary tumor model. We find that basal-like cancer cells resemble the alveolar lineage that is specified upon pregnancy and encompass the acquisition of an aberrant post-lactation developmental program of involution that triggers remodeling of the tumor microenvironment and metastatic dissemination. This involution mimicry is characterized by a highly interactive multicellular network, with involution cancer-associated fibroblasts playing a pivotal role in extracellular matrix remodeling and immunosuppression. Our results may partially explain the increased risk and poor prognosis of breast cancer associated with childbirth.</p
- âŠ