シナプス膜への特異的セロトニン結合に及ぼすフィルターの影響

In the present study, the authors carried out some basic examinations of specific (3H)-5-Hydroxytryptamine (5-HT, serotonin) receptor binding assay. The most adequate incubation condition for (3H)-5-HT binding assay was at 0°C for 10 to 30 min. Scatchard plot of (3H)-5-HT binding using a Millpore filter and a Glass filter drew biphasic and monophasic, respectively. It is suggested that filter may affect on specific (3H)-5-HT binding. Therefore, the characteristics of filter should be taken into consideration in (3H)-5-HT binding assay