Isolation of intact mesophyll cells from the leaves of higher plants

A total of 146 species of angiosperms belonging to 35 taxonomically diverse families were screened for the isolation of living mesophyll cells from the leaves. Seventy-three species belonging to 22 families, on mild maceration in mortar with the isolation medium (pH 5.8) containing 0.7 M mannitoJ, 2 mM EDTA, 5 mM MgCl2, 5 mM K2HPO4 and 1 mM NaNO3, followed by fractional centrifugation, yielded intact mesophyll cells as seen under a research microscope. The high frequency of cell release, associated with the high percentage recovery of chlorophyll in cells was a common feature of most of the plant species examined by us. Nearly 87% of the chlorophyll present in the leaf could be recovered from the isolated cells in Dolichos lablab. The isolated cells retained active photosynthetic carbon metabolism as evidenced by high rates of ferricyanide reduction as well as carbon assimilation

Completion of a Dislocated Metric Space

We provide a construction for the completion of a dislocated metric space (abbreviated d-metric space); we also prove that the completion of the metric associated with a d-metric coincides with the metric associated with the completion of the d-metric

¿Es posible el diagnóstico foliar mediante técnicas "in vivo"?

Se describe el uso de una técnica “in vivo” para el diagnóstico foliar. A igualdad de clorofila por área, la cantidad de citocromo f por área es menor en la carencia de hierro que en otras deficiencias. La medida de la concentración de citocromo se realiza sobre hojas intactas, siguiendo los cambios en Absorbancia a 554 nm tras fotooxidación con luz roja.Peer reviewe