Effect of Imidacloprid and Triadimefon on microbial phosphatase, protease and urease enzyme activities in tomato (Lycopersicon esculentum sp.) cultivated soil

The laboratory studies were conducted to resolute the effects of Imidacloprid (insecticide) and Triadimefon (fungicide) singly and in combination on enzymatic activities of soil microorganisms in tomato cultivated soils at different concentrations of 0.2, 0.5 and 0.7 kg per hectare. It was observed that phosphatase, protease and urease activities were elevated noteworthy at 0.5kg per hectare after 10 days of incubation. At lower concentration the activities of these enzymes showed no significant difference from that of control. The combination of the two pesticides led to a pronounced decline of phosphatase and protease enzyme activities at higher concentration. Triadimefon had no effect on urease activity at low concentrations whereas at 0.5 and 0.7 concentrations there was a significant increase when compared to control

Microbial polyhydroxybutyrate production by using cheap raw materials as substrates

Bioplastic, Polyhydroxybutyrate (PHB) is well known for it’s environmental friendliness and complete decomposition into water and carbon dioxide by microorganisms. The main drawback of PHB commercialization is it’s high production cost which is 10 times higher than that of synthetic plastic. So, the present research work mainly focussed on the fermentative production of PHB by Bacillus amyloliquifaciens and Nocardiopsis potens using low cost raw materials like Molasses, wheat bran, rice bran, ragi bran, jambul seed powder, orange peel and whey as substrates. Bacillus amyloliquifaciens and Nocardiopsis potens gives maximum PHB 16.5 µg/ ml and 26.8 µg/ ml respectively in the medium containing molasses and wheat bran as a substrates. Further the functional groups of extracted PHB were confirmed by Fourier transform infrared spectroscopy

Effect of Imidacloprid and Triadimefon on microbial phosphatase, protease and urease enzyme activities in tomato (Lycopersicon esculentum sp.) cultivated soil

The laboratory studies were conducted to resolute the effects of Imidacloprid (insecticide) and Triadimefon (fungicide) singly and in combination on enzymatic activities of soil microorganisms in tomato cultivated soils at different concentrations of 0.2, 0.5 and 0.7 kg per hectare. It was observed that phosphatase, protease and urease activities were elevated noteworthy at 0.5kg per hectare after 10 days of incubation. At lower concentration the activities of these enzymes showed no significant difference from that of control. The combination of the two pesticides led to a pronounced decline of phosphatase and protease enzyme activities at higher concentration. Triadimefon had no effect on urease activity at low concentrations whereas at 0.5 and 0.7 concentrations there was a significant increase when compared to control