Fluorescent copper(II) complexes: The electron transfer mechanism, interaction with bovine serum albumin (BSA) and antibacterial activity

Dinuclear copper(II) complexes with formula [Cu2(L)2(N3)2] (1) and [Cu2(L)2(NCS)2] (2) HL = (1-[(3-methyl-pyridine-2-ylimino)-methyl]-naphthalen-2-ol) were synthesized by controlling the molar ratio of Cu(OAC)2·6H2O, HL, sodium azide (1) and ammonium thiocyanate (2). The end on bridges appear exclusively in azide and thiocyanate to copper complexes. The electron transfer mechanism of copper(II) complexes is examined by cyclic voltammetry indicating copper(II) complexes are Cu(II)/Cu(I) couple. The interactions of copper(II) complexes towards bovine serum albumin (BSA) were examined with the help of absorption and fluorescence spectroscopic tools. We report a superficial solution-based route for the synthesis of micro crystals of copper complexes with BSA. The antibacterial activity of the Schiff base and its copper complexes were investigated by the agar disc diffusion method against some species of pathogenic bacteria (Escherichia coli, Vibrio cholerae, Streptococcus pneumonia and Bacillus cereus). It has been observed that the antibacterial activity of all complexes is higher than the ligand

Synthesis of biologically active cadmium (II) complex with tridentate N2O donor Schiff base: DFT study, binding mechanism of serum albumins (bovine, human) and fluorescent nanowires

The photophysical properties of luminescent tetra-coordinated cadmium (II) complex formulated as [Cd(L)Cl],HL = (1-[(3-methyl-pyridine-2-ylimino)-methyl]-naphthalen-2-ol) were synthesized and characterized by analytical and spectroscopic methods. The density function theory calculations are used to investigate the electronic structures of the ligand and its complex. The interactions of cadmium (II) complex towards bovine serum albumin (BSA) and human serum albumin (HSA) were investigated using absorption and fluorescence spectroscopic techniques at pH 7.4. The quenching constants, binding constants and number of binding sites were determined by fluorescence quenching method. The calculated thermodynamic parameters (ΔG, ΔH, and ΔS) confirmed that the binding reaction is mainly entropy-driven and hydrophobic forces played an important role in the reaction. Here, we proposed a new synthetic procedure for the preparation of BSA and HSA with cadmium complex nanowires. The scanning electron microscopy images show that BSA and HSA with cadmium complex product are wire-like in structure. The complex shows enhanced antibacterial activity compared with the free ligand and standard antibiotic chloramphenicol. Antioxidant studies showed that the complex has significant antioxidant activity against DPPH. The obtained IC50 value of the DPPH activity for complex (IC50 = 138 μg/ml) showed excellent scavenging property compared to standard ascorbic acid

Infectious mononucleosis due to epstein-barr virus infection in children: A profile from eastern India

Objective: The objective of this study is to delineate the clinical and laboratory profile of infectious mononucleosis due to Epstein-Barr virus (EBV) infection in children admitted to tertiary care teaching hospitals. Materials and Methods: Retrospective observational multicentric analysis of clinical and laboratory features of children between 1 month to 12 years with a diagnosis of infectious mononucleosis due to EBV infection confirmed by positive serology over a 12-month period after seeking approval from the Institutional Ethics Committee. Results: Out of 66 children screened, 53 were included in final analysis. The majority were aged between 5 and 8 years with male: female ratio of 1.2:1. Most presentations were during the monsoon months. The common clinical features were fever (100%), splenomegaly (86.7%), and cervical lymphadenopathy (73.5%) in contrast to the classical triad of fever, sore throat, and generalized lymphadenopathy described in the literature. There were no age differences in clinical findings except for generalized and cervical lymphadenopathy and hepatomegaly which were commoner in 9–12 years age band. Although the incidence of common findings matched with previously published studies, there were some notable differences. While frequencies of upper eyelid edema, epitrochlear lymphadenopathy, and splenomegaly were more, those of rash and sore throat were less. Lymphocytosis and presence of atypical lymphocytes were relatively less common in our series. All children recovered. Conclusions: This multicentric study on profiling childhood infectious mononucleosis, possibly first of its kind from Eastern India, has documented clinical and laboratory features associated with this condition. These data can serve as a reference for future studies