109 research outputs found

    Bridging sd1 molecular knowledge with recent breeding strategies for the improvement of traditional rice varieties - a japonica case-study

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    The rice semidwarfing gene, sd1, also known as the “green revolution gene”, has been studied intensively due to its contribution to the increase of crop production. Although sd1 breeding was extensively applied since the 1960s, the recent advances in the molecular basis of this gene alloweddesigning a more precise breeding strategy - marker assisted backcrossing (MAB) - to track sd1 introgression in two traditional rice varieties. For selection of sd1 plants we first confirmed the efficiency of specific markers based on Os200 x 2 gene sequence. Background selection was alsoperformed with the help of microsatellites markers (SSR) and a total of 7 breeding lines were recovered containing a higher percentage of recurrent parent genome (RPG). Analysis of Covariance (ANCOVA) using mean progenitor plant height as covariate was performed to compare several agronomic and quality-related parameters in two different environments. The results suggest that plant height differs significantly between the two environments F(1, 220) = 155.336; p < 0.001. From the total variability ofplant height we could conclude that 73% is due to the genotype, while 10.4% depends on the environment. In addition, the percentage of RPG seems negatively correlated with plant height (p < 0.005). MAB and background selection thus revealed as useful tools to assist breeding forsemidwarfism in traditional rice varieties

    Genetic Parameters of Some Characters and Their Correlation with Rice Grain Yield in Relation to the Plant Adaptability to Semi-Deep Stagnant Flooding Condition

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    Stagnant flooding (SF) is a major problem in floodprone rice ecosystem where the depth of flood water ranging from 20-50 cm in the entire seasons. In Indonesia this kind of water stress can be found in swampy basin area or Rawa Lebak. A study was conducted to determine the genetic parameters related to rice plant adaptability to SF. Eighteen rice genotypes including the recently developed 6 pairs of SUB1 near isogenic lines (NILs) of widely planted varieties and checks were tested under 50 cm depth of SF in the 2009 wet season at Los Banos, Philippines. The results showed that most of genotypes showed different responses on morphological and agronomical characters under SF and normal conditions. Most of the SUB1 NILs had lower grain yields than their respective parents. This was because SUB1 suppressed shoot elongation and less restoring contact with the air. Some SUB1 lines such as PSBRc68 and IR70181-32, however, produced high grain yields under this condition. Under SF condition, rice genotypes required shoot elongation to allow restoring contact with the air, therefore the traits that related to growth attributes such as shoot weight, leaf weight, LAI, stem diameter, plant high, and tiller number were strongly correlated with the grain yields. These traits also had broad genetic variability, high heritability, and strong correlation with grain yields. Selection under real stress SF conditions was effective to obtain high yielding genotypes and also tolerant

    The molecularization of public sector crop breeding: progress, problems, and prospects

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    Molecular markers and genetic maps are available for most important food crops. Marker-trait associations have been established for a diverse array of traits in these crops, and research on marker/quantitative trait loci (QTL) validation and refinement is increasingly common. Researchers are now routinely using candidate gene-based mapping and genome-wide linkage disequilibrium and association analysis in addition to classical QTL mapping to identify markers broadly applicable to breeding programs. Marker-assisted selection (MAS) is practiced for enhancing various host plant resistances, several quality traits, and a number of abiotic stress tolerances in many well-researched crops. Markers are also increasingly used to transfer yield or quality- enhancing QTL alleles from wild relatives to elite cultivars. Large-scale MAS-based breeding programs for crops such as rice, maize, wheat, barley, pearl millet, and common bean have already been initiated worldwide. Advances in "omics" technologies are now assisting researchers to address complex biological issues of significant agricultural importance: modeling genotype-by-environment interaction; fine-mapping, cloning, and pyramiding of QTL; gene expression analysis and gene function elucidation; dissecting the genetic structure of germplasm collections to mine novel alleles and develop genetically structured trait-based core collections; and understanding the molecular basis of heterosis. The challenge now is to translate and integrate this knowledge into appropriate tools and methodologies for plant breeding programs. The role of computational tools in achieving this is becoming increasingly important. It is expected that harnessing the outputs of genomics research will be an important component in successfully addressing the challenge of doubling world food production by 2050

    Association mapping of stigma and spikelet characteristics in rice (Oryza sativa L.)

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    Stigma and spikelet characteristics play an essential role in hybrid seed production. A mini-core of 90 accessions developed from USDA rice core collection was phenotyped in field grown for nine traits of stigma and spikelet and genotyped with 109 DNA markers, 108 SSRs plus an indel. Three major clusters were built upon Rogers’ genetic distance, indicative of indicas, and temperate and tropical japonicas. A mixed linear model combining PC-matrix and K-matrix was adapted for mapping marker-trait associations. Resulting associations were adjusted using false discovery rate technique. We identified 34 marker-trait associations involving 22 SSR markers for eight traits. Four markers were associated with single stigma exsertion (SStgE), six with dual exsertion (DStgE) and five with total exsertion. RM5_Chr1 played major role indicative of high regression with not only DStgE but also SStgE. Four markers were associated with spikelet length, three with width and seven with L/W ratio. Numerous markers were co-associated with multiple traits that were phenotypically correlated, i.e. RM12521_Chr2 associated with all three correlated spikelet traits. The co-association should improve breeding efficiency because single marker could be used to assist breeding for multiple traits. Indica entry 1032 (cultivar 50638) and japonica entry 671 (cultivar Linia 84 Icar) with 80.65 and 75.17% of TStgE, respectively are recommended to breeder for improving stigma exsertion

    Rac1 Is Required for Pathogenicity and Chm1-Dependent Conidiogenesis in Rice Fungal Pathogen Magnaporthe grisea

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    Rac1 is a small GTPase involved in actin cytoskeleton organization and polarized cell growth in many organisms. In this study, we investigate the biological function of MgRac1, a Rac1 homolog in Magnaporthe grisea. The Mgrac1 deletion mutants are defective in conidial production. Among the few conidia generated, they are malformed and defective in appressorial formation and consequently lose pathogenicity. Genetic complementation with native MgRac1 fully recovers all these defective phenotypes. Consistently, expression of a dominant negative allele of MgRac1 exhibits the same defect as the deletion mutants, while expression of a constitutively active allele of MgRac1 can induce abnormally large conidia with defects in infection-related growth. Furthermore, we show the interactions between MgRac1 and its effectors, including the PAK kinase Chm1 and NADPH oxidases (Nox1 and Nox2), by the yeast two-hybrid assay. While the Nox proteins are important for pathogenicity, the MgRac1-Chm1 interaction is responsible for conidiogenesis. A constitutively active chm1 mutant, in which the Rac1-binding PBD domain is removed, fully restores conidiation of the Mgrac1 deletion mutants, but these conidia do not develop appressoria normally and are not pathogenic to rice plants. Our data suggest that the MgRac1-Chm1 pathway is responsible for conidiogenesis, but additional pathways, including the Nox pathway, are necessary for appressorial formation and pathogenicity

    Genomic Diversity and Introgression in O. sativa Reveal the Impact of Domestication and Breeding on the Rice Genome

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    The domestication of Asian rice (Oryza sativa) was a complex process punctuated by episodes of introgressive hybridization among and between subpopulations. Deep genetic divergence between the two main varietal groups (Indica and Japonica) suggests domestication from at least two distinct wild populations. However, genetic uniformity surrounding key domestication genes across divergent subpopulations suggests cultural exchange of genetic material among ancient farmers.In this study, we utilize a novel 1,536 SNP panel genotyped across 395 diverse accessions of O. sativa to study genome-wide patterns of polymorphism, to characterize population structure, and to infer the introgression history of domesticated Asian rice. Our population structure analyses support the existence of five major subpopulations (indica, aus, tropical japonica, temperate japonica and GroupV) consistent with previous analyses. Our introgression analysis shows that most accessions exhibit some degree of admixture, with many individuals within a population sharing the same introgressed segment due to artificial selection. Admixture mapping and association analysis of amylose content and grain length illustrate the potential for dissecting the genetic basis of complex traits in domesticated plant populations.Genes in these regions control a myriad of traits including plant stature, blast resistance, and amylose content. These analyses highlight the power of population genomics in agricultural systems to identify functionally important regions of the genome and to decipher the role of human-directed breeding in refashioning the genomes of a domesticated species
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