Uslovija rosta cestod iz roda Psudophyllidea in vitro

The review covers previous publications of Smyth dealing with cultivation of larval Cestode in vitro. Plecercoides were rearing in axenic conditions in vitro and obtained after 72 hours a mature from of Schistocephalus solidus. The plerocercoids were kept in a tube made of half permeable membrane which was placed in a vessel with fluent medium - horse serum; above vessel was heated to a temperature of 40° C being all the time shaked. The rearing of the coracides, out of Schistocephalus solidus ova obtained in vitro, procecded in water with a temperature or 25° C. The incubation of the coracides took place after 8 days. When the culture was trated with sun rays for several hours the process of the ova operculi opening was accelerated. The best results in cultivating plerocercoids Diphyllobothrium dendriticum in vitro up to a mature from was obtained on a medium containing an extract of duck embryo together with serum, monosacharides and albuminous hydrolysates. The plerocercoids after 6 days of breeding attained the stadium of late gametogenesis and shoved a defective somatic development. Any enrichment of the medium in nutritive substances failed to evoke further progress in the plerocercoids development - viz. formation of vitellous glands. In order to check whether the methods chosen for the development of tapeworms reared in vitro have been correct, cytologie, histological and histochemical tests have been performed. Cultivation in vitro is a contribution to the better understanding of relations and dependency existing between the parasite and its host

Gemaggljutacionnaja reakcija u ljudejj s invaziejj Taenia saginata

Using the hemagglutination test the authors came to the conclusion that a positive result is a transitory phenomenon related to the presence of the parasite in old cases or to the deinfestation procedure. The removal of Taenia from the organism results - in uncomplicated cases - in the loss of positive reaction

Metabolic syndrome is associated with similar long-term prognosis in non-obese and obese patients. An analysis of 45 615 patients from the nationwide LIPIDOGRAM 2004-2015 cohort studies

Aims We aimed to evaluate the association between metabolic syndrome (MetS) and long-term all-cause mortality. Methods The LIPIDOGRAM studies were carried out in the primary care in Poland in 2004, 2006 and 2015. MetS was diagnosed based on the National Cholesterol Education Program, Adult Treatment Panel III (NCEP/ATP III) and Joint Interim Statement (JIS) criteria. The cohort was divided into four groups: non-obese patients without MetS, obese patients without MetS, non-obese patients with MetS and obese patients with MetS. Differences in all-cause mortality was analyzed using Kaplan-Meier and Cox regression analyses. Results 45,615 participants were enrolled (mean age 56.3, standard deviation: 11.8 years; 61.7% female). MetS was diagnosed in 14,202 (31%) by NCEP/ATP III criteria, and 17,216 (37.7%) by JIS criteria. Follow-up was available for 44,620 (97.8%, median duration 15.3 years) patients. MetS was associated with increased mortality risk among the obese (hazard ratio, HR: 1.88 [95% CI, 1.79-1.99] and HR: 1.93 [95% CI 1.82-2.04], according to NCEP/ATP III and JIS criteria, respectively) and non-obese individuals (HR: 2.11 [95% CI 1.85-2.40] and 1.7 [95% CI, 1.56-1.85] according to NCEP/ATP III and JIS criteria respectively). Obese patients without MetS had a higher mortality risk than non-obese patients without MetS (HR: 1.16 [95% CI 1.10-1.23] and HR: 1.22 [95%CI 1.15-1.30], respectively in subgroups with NCEP/ATP III and JIS criteria applied). Conclusions MetS is associated with increased all-cause mortality risk in non-obese and obese patients. In patients without MetS obesity remains significantly associated with mortality. The concept of metabolically healthy obesity should be revised