Ochrona środowiska przed elektromagnetycznym promieniowaniem niejonizującym. Telekomunikacja i Techniki Informacyjne, 2010,nr 3-4

Opisano oddziaływanie pola elektromagnetycznego pochodzącego z różnych źródeł na organizm człowieka i skalę zagrożeń dla zdrowia. Przeanalizowano zasady określania wartości dopuszczalnych pola w Polsce i na świecie dla ogółu społeczeństwa i osób pracujących w otoczeniu źródeł pola. Przedstawiono działalność Instytutu Łączności w dziedzinie kompatybilności elektromagnetycznej i pomiarów pola dla celów BHP i ochrony środowiska

Enhancing metabarcoding efficiency and ecological insights through integrated taxonomy and DNA reference barcoding: A case study on beach meiofauna

Molecular techniques like metabarcoding, while promising for exploring diversity of communities, are often impeded by the lack of reference DNA sequences available for taxonomic annotation. Our study explores the benefits of combining targeted DNA barcoding and morphological taxonomy to improve metabarcoding efficiency, using beach meiofauna as a case study. Beaches are globally important ecosystems and are inhabited by meiofauna, microscopic animals living in the interstitial space between the sand grains, which play a key role in coastal biodiversity and ecosystem dynamics. However, research on meiofauna faces challenges due to limited taxonomic expertise and sparse sampling. We generated 775 new cytochrome c oxidase I DNA barcodes from meiofauna specimens collected along the Netherlands' west coast and combined them with the NCBI GenBank database. We analysed alpha and beta diversity in 561 metabarcoding samples from 24 North Sea beaches, a region extensively studied for meiofauna, using both the enriched reference database and the NCBI database without the additional reference barcodes. Our results show a 2.5-fold increase in sequence annotation and a doubling of species-level Operational Taxonomic Units (OTUs) identification when annotating the metabarcoding data with the enhanced database. Additionally, our analyses revealed a bell-shaped curve of OTU richness across the intertidal zone, aligning more closely with morphological analysis patterns, and more defined community dissimilarity patterns between supralittoral and intertidal sites. Our research highlights the importance of expanding molecular reference databases and combining morphological taxonomy with molecular techniques for biodiversity assessments, ultimately improving our understanding of coastal ecosystems

Towards transdisciplinary decision-support processes in fisheries: experiences and recommendations from a multidisciplinary collective of researchers

The shifting policy focus towards Ecosystem Based Management in Fisheries (EBFM) requires the integration of knowledge and disciplines and the engagement of stakeholders to support decision-making processes. Scientists contribute to this through (i) participatory research projects, (ii) stakeholder partnerships and (iii) institutional scientific advice processes. Understanding the role of scientists in such processes, the nature of the interactions between scientists, stakeholders and managers in knowledge integration and the link between science and policy is an emerging field of research addressing transdisciplinary challenges. In 2018, Ifremer-UMR AMURE organized the workshop ‘Science, Partnership and Decision-support in Fisheries’ bringing together international scientists from natural and social sciences to conduct a review based on twenty concrete case studies. Findings indicate that science-stakeholder-manager partnerships for decision-support in fisheries can play an essential role in the transition to EBFM. To foster this transition, eight recommendations are presented that cover the roles of the different participants, the expectations of partnerships, capacity building, the integration of the social sciences, and funding structures. Further, it is recommended that future research and innovation framework programmes into sustainable fisheries and other ocean uses should explicitly include mechanisms to foster transdisciplinary approaches and the development of best practices. Building-up networks and developing reflexive approaches to review experiences and practices for transdisciplinary approaches in EFBM decision-support will contribute to design the next generation transdisciplinary platforms and generating actionable knowledge towards EBFM

Workshop on Stakeholder Engagement Strategy (WKSHOES)

The ICES Workshop on Stakeholder Engagement Strategy (WKSHOES) met online 22-24 June 2021 with the objective to organize the background information needed for SCICOM and ACOM to develop a formal ICES Stakeholder Engagement Strategy. Stakeholder engagement has taken an increasingly important role in ICES. There was a strong consensus in the workshop that stakeholder engagement is essential to ICES’ work, as has been captured by the most recent ICES Strategic, Science, and Advisory Plans. The question is how to do it best. While WKSHOES recognized the essential nature of stakeholder engagement for addressing environmental challenges, understanding human impacts and values, the group discussed the valid concern that if stakeholder engagement is done incorrectly, it could compromise the perceived objectivity of ICES science and its independence. Workshop participants challenged the idea of objective or “pure” science, but also recognized the practical need to have ICES advice be transparent and science-based. Participants also understand that when providing advice, tradeoffs have to be made that are informed by the different weights that stakeholders place on various management objectives. A key question related to a potential stakeholder engagement strategy is “Who is a stakeholder?” This report discusses several definitions and roles. A central challenge for ICES is recognizing that the network of stakeholders is a subset of the people impacted by ICES science and advice. The Stakeholder Engagement Strategy should therefore primarily focus on ensuring that people who are part of the ICES network have clear roles and responsibilities and that ICES performs its work fairly and transparently. However, participants also recognize the need to increase opportunities for diverse resource users and citizens to have clear avenues to engage with the different aspects of the ICES network. Considering and promoting diversity and inclusion and avoiding implicit bias are crucial in this process. It is also essential to define clear goals for stakeholder engagement in general, and tangible objectives for each engagement activity in particular. Engagement objectives already formulated in various ICES documents are outlined in this report, as well as recommendations for guiding principles that provide the overarching frame of reference for engagement. This Report serves as the primary output from WKSHOES, and represents the knowledge and opinions of workshop participants. WKSHOES recommends that in order to both complete the development of the strategy and conduct successful stakeholder engagement about it, a suite of communication activities is needed that should best be coordinated from a central contact point within ICES. WKSHOES recommends that after ACOM and SCICOM draft the strategy and obtain input from the ICES Council, a diversity of stakeholders should be invited to provide input on the WKSHOES report and the development of the Engagement Strategy. ICES should solicit input from stakeholders by early 2022 to agree on the contents of the Strategy and how it will be implemented. WKSHOES also recommends that a future Expert Group address the ongoing need to develop and communicate effective stakeholder engagement methods within the ICES network.Peer reviewe

Avaliação de um treinamento estático de carga parcial de peso Evaluation of partial static weight load training

O treinamento da marcha com carga parcial é comum na prática do fisioterapeuta, entretanto, na área hospitalar, o tempo para esse treinamento é limitado, durando somente o tempo da internação. Dessa forma, este estudo tem como objetivo verificar o efeito do treinamento prévio de carga parcial de peso, em voluntárias sem lesões ortopédicas buscando direcionar esse treinamento para pacientes submetidos a cirurgias ortopédicas nos membros inferiores. Participaram deste estudo 32 voluntárias sem alterações posturais ou história de traumas nos membros inferiores. O treinamento de carga parcial foi realizado distribuindo-se 20% da massa corporal total no membro inferior direito em uma balança digital antropométrica. As voluntárias foram divididas em dois grupos, sendo que o grupo I repetiu o treinamento 6 vezes e o grupo II, 12 vezes. Os dados da distribuição estática da carga foram coletados em uma plataforma de pressão Matscan-Tekscan® e comparados no pré e pós-treinamento. Foi verificado que houve diferença significativa no treinamento repetido 12 vezes (pGait training with partial load is common in a physical therapist practice; however, in the hospital environment, the time for this training is limited, lasting only for the time of hospitalization. Therefore, this study was aimed to check on the effects of previous partial weight load training in volunteers without orthopaedic injuries seeking to indicate it to patients submitted to orthopaedic surgeries on lower limbs. 32 volunteers showing no postural changes or history of trauma on lower limbs were included in this study. The partial load training was carried out by distributing 20% of the total body mass on the right lower limb in an anthropometric digital scale. The volunteers were divided into two groups, with group I repeating the drill 6 times, and group II 12 times. Data about the static load distribution were collected by a pressure platform Matscan-Tekscan® and compared on the pre- and post-training periods. A significant difference was found for 12-repetitions drills (p<0.015). Pre-training with partial weight load was shown to be effective to be applied on patients during hospitalization period, because with only a single drill repeated 12 times, partial load learning was noticed

Non-invasive Prenatal Diagnosis of Feto-Maternal Platelet Incompatibility by Cold High Resolution Melting Analysis

Gahan P., Fleischhacker M., Schmidt B. (eds).Fetal and Neonatal alloimmune thrombocytopenia (FNAIT) is a condition which could occur when pregnant women develop an alloimmunization against paternally inherited antigens of the fetal platelets. Approximately 80 % of FNAIT cases are caused by anti-HPA-1a, about 15 % by anti-HPA-5b and 5 % by other HPA antibodies. Only 2 % of the total population is HPA-1a negative (HPA-1b1b). The HPA-1a allele differs by one single nucleotide from HPA-1b allele, yet it represents around 27 % of total severe thrombocytopenias. HPA-1 was studied in serum cDNA from 12 volunteer pregnant women to determine their HPA-1 genotype by HRM (high resolution melting) PCR. When an homozygous HPA-1 gene was detected in a mother, a COLD HRM was performed to determine whether or not the fetal genotype differs from the mother’s. The differences in the melting curve shapes allow us to accurately distinguish the three pregnants genotypes. The fetal heterozygous genotype of homozygous pregnant women was correctly detected by COLD PCR HRM in maternal serum. HPA-1 genotyping by HRM may be a useful aproach for genotyping all pregnant women in inexpensively. Moreover, when HPA-1 homozygosis was detected in a pregnant woman, fetal heterozygosis may be diagnosed by COLD HRM to select pregnancies for preventive monitoring.Peer reviewe

Family study of a novel mutation of mucopolysaccharidosis type VI with a severe phenotype and good response to enzymatic replacement therapy: Case report.

Mucopolysaccharidosis type VI (MPS VI) or Maroteaux-Lamy syndrome is produced by the deficiency of the enzyme arylsulfatase B, responsible for the hydrolysis of N-acetyl-D-galactosamine, chondroitin sulfate, and dermatan sulfate. A 3-year-old male with Moroccan origins is the index case. He had healthy consanguineous parents and 4 healthy brothers and sisters. The patient showed a wide spectrum of symptoms including skeletal dysplasia and short stature with elevated glycosaminoglycans (GAGs) in urine. GAGs were quantified by spectrometry method with 1,9-dimethylen blue in 24-hour urine samples. The qualitative analysis of urine GAGs was obtained by thin-layer chromatography to determine the predominant presence of dermatan sulfate. The activities of both arylsulfatase B and beta-galactosidase as well as genetic studies were performed in dried blood spots. The genetic study was performed with deoxyribonucleic acid by massive sequencing a of lisosomal storage diseases. Results showed a new mutation c.263A > C with the severe phenotype in homozygous in the patient. The familiar study of ARSB and GLB1 genes presented some asymptomatic SNPs but with a discrete decrease in the activity of arylsulfatase B and beta-galactosidase. After an early detection by pediatricians, and both enzymatic and genetic confirmation, the patient had a good response to substitutive enzymatic treatment with galsulfase. Mucoplysaccharidosis type VI is an autosomal recessive rare disease characterized by a lysosomal storage disorder. Although a number of mutations have been already associated to the disease, we have found a new mutation located in the arylsulfatase B enzyme gene. We have described that this mutation is the ultimate cause of a severe presentation of the disease

Science, partnership and decision support processes in fisheries: sharing experiences and practices and identifying Lessons learnt from an interdisciplinary perspective

Implementation of Ecosystem Based Managementin fisheries has led to higher integration of knowledge in advices and higher engagement of stakeholders in the decision support Process. As part of the process, fisheries scientists contributein different ways and at different levels to decision support and experienced partnership approaches in the context of research projects or institutional decision support. Role of scientists in the process is howevernot trivial to define and operationalize. Several pitfalls can occur that scientists may have experienced through partnership projects or in the expert assessments done for authorities. A workshop was organized from 15 to 17 January 2018 as part of the ScipaDeproject of the Cluster of Excellence('Labex') funded by the French 'Investment for the future' program and supported by the French Ministry of Research and Education. It brought together biologists, economists and social and political scientists involved in decision support processesand partnership approaches in fisheries at European level to share experiences and views across case studies, disciplines and projects. Context, content and lessons learnt from concrete experiences of bio-economic impact assessment of fisheries management scenarios provided in different case studies were reviewed and the links between scientists-stakeholders-decision makers in the decision support process in fisheries was explored through discussions and SWOT analyses. An Australian case was also reported to put into perspective the approaches and lessons learnt described at the European scale with respect to the institutionalized partnership platform for decision making used in Australian. This report provides information on the SciPaDe project, the participants to the workshop, the content of the workshop and the main perspectives and tasks identified. The workshop underlined the need to continue and better structure networks around interdisciplinary approaches, to share our vision of the role of scientists in decision support process, our experiences, methods and recommendationsand to disseminate lessons learnt from analysis of practices in integrated science decision support in fisheries

Science, partnership and decision support processes in fisheries: sharing experiences and practices and identifying Lessons learnt from an interdisciplinary perspective

Implementation of Ecosystem Based Management in fisheries has led to higher integration of knowledge in  advices  and  higher  engagement  of  stakeholders  in  the  decision  support  Process.  As  part  of  the process,  fisheries scientists contribute  in different ways and at different levels to decision support and experienced partnership approaches in the context of research projects or institutional decision support. Role of scientists in  the process is however  not trivial to define and operationalize. Several pitfalls can occur   that   scientists   may   have   experienced   through   partnership   projects   or   in   the   expert assessments done for authorities. A workshop  was organized from 15 to 17 January 2018  as part of  the ScipaDe  project of the  Cluster of Excellence  ('Labex') funded by the French 'Investment for the future' program and supported by the French Ministry of Research and Education. It brought together biologists, economists  and  social  and  political  scientists  involved  in  decision  support  processes  and  partnership approaches in fisheries  at European level  to share experiences and views  across case studies, disciplines and  projects.  Context,  content  and  lessons  learnt  from  concrete  experiences  of  bio-economic  impact assessment of fisheries management scenarios provided in different case studies were reviewed and the links  between  scientists-stakeholders-decision  makers  in  the  decision  support  process  in  fisheries  was explored  through  discussions  and  SWOT  analyses.  An  Australian  case  was  also  reported  to  put  into perspective  the  approaches  and  lessons  learnt  described  at  the  European  scale  with  respect  to  the institutionalized partnership platform for decision making used in Australian. This report provides information on the SciPaDe project, the participants to the workshop, the content of the  workshop  and  the  main  perspectives  and  tasks  identified.  The  workshop  underlined  the  need  to continue and better structure networks around interdisciplinary approaches,  to share our vision of the role of scientists  in  decision support process, our experiences, methods and recommendations  and  to disseminate lessons learnt from analysis of practices in integrated science decision support in fisheries.

Towards transdisciplinary decision-support processes in fisheries: experiences and recommendations from a multidisciplinary collective of researchers

The shifting policy focus towards Ecosystem Based Management in Fisheries (EBFM) requires the integration of knowledge and disciplines and the engagement of stakeholders to support decision-making processes. Scientists contribute to this through (i) participatory research projects, (ii) stakeholder partnerships and (iii) institutional scientific advice processes. Understanding the role of scientists in such processes, the nature of the interactions between scientists, stakeholders and managers in knowledge integration and the link between science and policy is an emerging field of research addressing transdisciplinary challenges. In 2018, Ifremer-UMR AMURE organized the workshop ‘Science, Partnership and Decision-support in Fisheries’ bringing together international scientists from natural and social sciences to conduct a review based on twenty concrete case studies. Findings indicate that science-stakeholder-manager partnerships for decision-support in fisheries can play an essential role in the transition to EBFM. To foster this transition, eight recommendations are presented that cover the roles of the different participants, the expectations of partnerships, capacity building, the integration of the social sciences, and funding structures. Further, it is recommended that future research and innovation framework programmes into sustainable fisheries and other ocean uses should explicitly include mechanisms to foster transdisciplinary approaches and the development of best practices. Building-up networks and developing reflexive approaches to review experiences and practices for transdisciplinary approaches in EFBM decision-support will contribute to design the next generation transdisciplinary platforms and generating actionable knowledge towards EBFM