Next generation sequencing analysis reveals a relationship between rDNA unit diversity and locus number in Nicotiana diploids

© 2012 Matyášek et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

GLADE: A galaxy catalogue for multimessenger searches in the advanced gravitational-wave detector era

We introduce a value-added full-sky catalogue of galaxies, named as Galaxy List for the Advanced Detector Era, or GLADE. The purpose of this catalogue is to (i) help identifications of host candidates for gravitational-wave events, (ii) support target selections for electromagnetic follow-up observations of gravitational-wave candidates, (iii) provide input data on the matter distribution of the local Universe for astrophysical or cosmological simulations, and (iv) help identifications of host candidates for poorly localized electromagnetic transients, such as gamma-ray bursts observed with the InterPlanetary Network. Both being potential hosts of astrophysical sources of gravitational waves, GLADE includes inactive and active galaxies as well. GLADE was constructed by cross-matching and combining data from five separate (but not independent) astronomical catalogues: GWGC, 2MPZ, 2MASS XSC, HyperLEDA, and SDSS-DR12Q. GLADE is complete up to dL=37+3−4Mpc in terms of the cumulative B-band luminosity of galaxies within luminosity distance dL, and contains all of the brightest galaxies giving half of the total B-band luminosity up to dL=91Mpc. As B-band luminosity is expected to be a tracer of binary neutron star mergers (currently the prime targets of joint GW+EM detections), our completeness measures can be used as estimations of completeness for containing all binary neutron star merger hosts in the local Universe

TEARA: Educational Treatment of Children with ASD, mediated through augmented reality

The treatments that used since the 1960 as educational proposals for children with autism spectrum disorder (ASD) are becoming obsolete over time. This research proposes an educational treatment for children with autism mediated through augmented reality called (TEARA), as a response to the challenges and constant change of a globalized world, which requires the establishment of new methods, strategies and treatments that allow improve the quality of life of these children with autism. The methodology was approached through a mixed, exploratory, descriptive, and purposeful study where a multidisciplinary team participated, we developed a training system called Hope, which reinforces and promotes teaching-learning processes, finally after several cycles of intervention, deep observation and the compilation of results, it was established that TEARA can be used by professionals, parents and people who accompany children with ASD.XI Workshop Innovación en Educación en Informática (WIEI)Red de Universidades con Carreras en Informátic

Analysis of the giant genomes of Fritillaria (Liliaceae) indicates that a lack of DNA removal characterizes extreme expansions in genome size.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.Plants exhibit an extraordinary range of genome sizes, varying by > 2000-fold between the smallest and largest recorded values. In the absence of polyploidy, changes in the amount of repetitive DNA (transposable elements and tandem repeats) are primarily responsible for genome size differences between species. However, there is ongoing debate regarding the relative importance of amplification of repetitive DNA versus its deletion in governing genome size. Using data from 454 sequencing, we analysed the most repetitive fraction of some of the largest known genomes for diploid plant species, from members of Fritillaria. We revealed that genomic expansion has not resulted from the recent massive amplification of just a handful of repeat families, as shown in species with smaller genomes. Instead, the bulk of these immense genomes is composed of highly heterogeneous, relatively low-abundance repeat-derived DNA, supporting a scenario where amplified repeats continually accumulate due to infrequent DNA removal. Our results indicate that a lack of deletion and low turnover of repetitive DNA are major contributors to the evolution of extremely large genomes and show that their size cannot simply be accounted for by the activity of a small number of high-abundance repeat families.Thiswork was supported by the Natural Environment ResearchCouncil (grant no. NE/G017 24/1), the Czech Science Fou nda-tion (grant no. P501/12/G090), the AVCR (grant no.RVO:60077344) and a Beatriu de Pinos postdoctoral fellowshipto J.P. (grant no. 2011-A-00292; Catalan Government-E.U. 7thF.P.)

Eje integrador educativo de las TICS : Caso de estudio: niños con trastorno del espectro autista

El estudio investigativo se realizó en las ciudades de Quito, Guayaquil y Cuenca pertenecientes a Ecuador en el periodo del primer semestre del año 2018 y se basó en el análisis del uso de las tecnologías de la información y comunicaciones TICS y su importancia en el acompañamiento ordinario como apoyo académico a las maestras integradoras en pro de conocer, aprender y mejorar la calidad del aprendizaje global y la experiencia del aula en estudiantes con necesidades educativas especiales (NEE) particularmente en niños diagnosticados con trastorno de espectro autista (TEA). Los objetivos de la investigación fueron determinar el rol, los beneficios, el aporte de las TICS a las maestras integradoras en la adaptación escolar, conocer si su uso permite obtener resultados positivos en procesos de enseñanza aprendizaje y plasmar directrices de fortalecimiento en el proceso de enseñanza aprendizaje de los niños con trastorno del espectro autista. Inicialmente se levantan datos a través de la generación de encuestas, luego se aplica instrumentos TICS y se evidencia su uso permitiendo que los niños diagnosticados mejoren su rendimiento escolar.Sociedad Argentina de Informática e Investigación Operativ

Eje integrador educativo de las TICS : Caso de estudio: niños con trastorno del espectro autista

El estudio investigativo se realizó en las ciudades de Quito, Guayaquil y Cuenca pertenecientes a Ecuador en el periodo del primer semestre del año 2018 y se basó en el análisis del uso de las tecnologías de la información y comunicaciones TICS y su importancia en el acompañamiento ordinario como apoyo académico a las maestras integradoras en pro de conocer, aprender y mejorar la calidad del aprendizaje global y la experiencia del aula en estudiantes con necesidades educativas especiales (NEE) particularmente en niños diagnosticados con trastorno de espectro autista (TEA). Los objetivos de la investigación fueron determinar el rol, los beneficios, el aporte de las TICS a las maestras integradoras en la adaptación escolar, conocer si su uso permite obtener resultados positivos en procesos de enseñanza aprendizaje y plasmar directrices de fortalecimiento en el proceso de enseñanza aprendizaje de los niños con trastorno del espectro autista. Inicialmente se levantan datos a través de la generación de encuestas, luego se aplica instrumentos TICS y se evidencia su uso permitiendo que los niños diagnosticados mejoren su rendimiento escolar.Sociedad Argentina de Informática e Investigación Operativ

GLADE+: An Extended Galaxy Catalogue for Multimessenger Searches with Advanced Gravitational-wave Detectors

We present GLADE+, an extended version of the GLADE galaxy catalogue introduced in our previous paper for multimessenger searches with advanced gravitational-wave detectors. GLADE+ combines data from six separate but not independent astronomical catalogues: the GWGC, 2MPZ, 2MASS XSC, HyperLEDA, and WISExSCOSPZ galaxy catalogues, and the SDSS-DR16Q quasar catalogue. To allow corrections of CMB-frame redshifts for peculiar motions, we calculated peculiar velocities along with their standard deviations of all galaxies having $B$-band magnitude data within redshift $z=0.05$ using the "Bayesian Origin Reconstruction from Galaxies" formalism. GLADE+ is complete up to luminosity distance $d_L=47^{+4}_{-2}$ Mpc in terms of the total expected $B$-band luminosity of galaxies, and contains all of the brightest galaxies giving 90\% of the total $B$-band and $K$-band luminosity up to $d_L\simeq 130$ Mpc. We include estimations of stellar masses and individual binary neutron star merger rates for galaxies with $W1$ magnitudes. These parameters can help in ranking galaxies in a given gravitational wave localization volume in terms of their likelihood of being hosts, thereby possibly reducing the number of pointings and total integration time needed to find the electromagnetic counterpart.Comment: 9 pages, 4 figures, accepted for publication in MNRA

Eje integrador educativo de las TICS : Caso de estudio: niños con trastorno del espectro autista

El estudio investigativo se realizó en las ciudades de Quito, Guayaquil y Cuenca pertenecientes a Ecuador en el periodo del primer semestre del año 2018 y se basó en el análisis del uso de las tecnologías de la información y comunicaciones TICS y su importancia en el acompañamiento ordinario como apoyo académico a las maestras integradoras en pro de conocer, aprender y mejorar la calidad del aprendizaje global y la experiencia del aula en estudiantes con necesidades educativas especiales (NEE) particularmente en niños diagnosticados con trastorno de espectro autista (TEA). Los objetivos de la investigación fueron determinar el rol, los beneficios, el aporte de las TICS a las maestras integradoras en la adaptación escolar, conocer si su uso permite obtener resultados positivos en procesos de enseñanza aprendizaje y plasmar directrices de fortalecimiento en el proceso de enseñanza aprendizaje de los niños con trastorno del espectro autista. Inicialmente se levantan datos a través de la generación de encuestas, luego se aplica instrumentos TICS y se evidencia su uso permitiendo que los niños diagnosticados mejoren su rendimiento escolar.Sociedad Argentina de Informática e Investigación Operativ

Stretching the Rules: Monocentric Chromosomes with Multiple Centromere Domains

The centromere is a functional chromosome domain that is essential for faithful chromosome segregation during cell division and that can be reliably identified by the presence of the centromere-specific histone H3 variant CenH3. In monocentric chromosomes, the centromere is characterized by a single CenH3-containing region within a morphologically distinct primary constriction. This region usually spans up to a few Mbp composed mainly of centromere-specific satellite DNA common to all chromosomes of a given species. In holocentric chromosomes, there is no primary constriction; the centromere is composed of many CenH3 loci distributed along the entire length of a chromosome. Using correlative fluorescence light microscopy and high-resolution electron microscopy, we show that pea (Pisum sativum) chromosomes exhibit remarkably long primary constrictions that contain 3-5 explicit CenH3-containing regions, a novelty in centromere organization. In addition, we estimate that the size of the chromosome segment delimited by two outermost domains varies between 69 Mbp and 107 Mbp, several factors larger than any known centromere length. These domains are almost entirely composed of repetitive DNA sequences belonging to 13 distinct families of satellite DNA and one family of centromeric retrotransposons, all of which are unevenly distributed among pea chromosomes. We present the centromeres of Pisum as novel ``meta-polycentric'' functional domains. Our results demonstrate that the organization and DNA composition of functional centromere domains can be far more complex than previously thought, do not require single repetitive elements, and do not require single centromere domains in order to segregate properly. Based on these findings, we propose Pisum as a useful model for investigation of centromere architecture and the still poorly understood role of repetitive DNA in centromere evolution, determination, and function

Genomic repeat abundances contain phylogenetic signal

A large proportion of genomic information, particularly repetitive elements, is usually ignored when researchers are using next-generation sequencing. Here we demonstrate the usefulness of this repetitive fraction in phylogenetic analyses, utilizing comparative graph-based clustering of next-generation sequence reads, which results in abundance estimates of different classes of genomic repeats. Phylogenetic trees are then inferred based on the genome-wide abundance of different repeat types treated as continuously varying characters; such repeats are scattered across chromosomes and in angiosperms can constitute a majority of nuclear genomic DNA. In six diverse examples, five angiosperms and one insect, this method provides generally well-supported relationships at interspecific and intergeneric levels that agree with results from more standard phylogenetic analyses of commonly used markers. We propose that this methodology may prove especially useful in groups where there is little genetic differentiation in standard phylogenetic markers. At the same time as providing data for phylogenetic inference, this method additionally yields a wealth of data for comparative studies of genome evolution