Structure of the polysaccharide O-antigen of Salmonella riogrande O:40 (group R) related to blood group A activity

NRC publication: Ye

Characterization of the antigenic O-polysaccharide produced by Escherichia coli serotype O:70

The structure of the antigenic O-polysaccharide (O-PS) produced by Escherichia coli serotype O:70 was determined by analysis of the chromatographically purified O-PS polymer prepared by mild hydrolysis of its aqueous phenol-extracted smooth-type somatic lipopolysaccharide. The O-PS is composed of d-glucose, d-galactose, d-fucose, 2-acetamido-2-deoxy-d-galactose, and 3-acetamido-3-deoxy-d-quinovose in a ratio of 1:1:1:1:1. From the use of DOC\u2013PAGE, methylation, Smith-type periodate oxidation, and \ub9H and \ub9\ub3C NMR spectroscopy, including 2D experiments, the O-PS was shown to be a polymer of a branched repeating pentasaccharide unit having the structurePeer reviewed: YesNRC publication: Ye

Structural determination of the O-antigenic polysaccharide of enteropathogenic Escherichia coli O103:H2

The structure of the antigenic O-polysaccharide isolated from the lipopolysaccharide produced by enterohemorrhagic Escherichia coli O103:H2 was determined and shown to be composed of D-glucose (1 part), 2-acetamido-2-deoxy-Dglucose (2 parts), 2-acetamido-2-deoxy-D-galactose (1 part), and 3-deoxy-3-(R)-3-hydroxybutyramido-D-fucose (1 part). From the results of methylation analysis, Smith-type periodate oxidation degradation studies, and the use of one- and twodimensional 1H and 13C NMR spectroscopy, the O-polysaccharide antigen was found to be an unbranched polymer of a repeating pentasaccharide unit having the following structure: ->2)-b-D-Glcp-(1->2)-b-D-Fucp3NBu-(1->6)-a-D-GlcpNAc- (1->4)-a-D-GalpNAc-(1->3)-b-D-GlcpNAc-(1->, where Bu is (R)-3-hydroxybutyramido.La structure du polysaccharide d\u2019antig\ue8ne O isol\ue9 du lipopolysaccharide produit par Escherichia coli O103:H2 ent\ue9ro-h\ue9morragique a \ue9t\ue9 d\ue9termin\ue9e et s\u2019est av\ue9r\ue9e compos\ue9e de D-glucose (1 partie), de 2-ac\ue9tamido-2-d\ue9oxy-D-glucose (2 parties), de 2-ac\ue9tamido-2-d\ue9oxy-D-galactose (1 partie) et de 3-d\ue9oxy-3-(R)-3-hydroxybutyramido-D-fucose (1 partie). Selon les r\ue9sultats d\u2019analyses de m\ue9thylation, d\u2019\ue9tudes de d\ue9gradation par l\u2019oxydation au periodate de Smith et de l\u2019utilisation de la spectroscopie 1H et 13C unidimensionnel et bidimensionnel, le polysaccharide O est apparu comme un polym\ue8re non branch\ue9 d\u2019unit\ue9s pentasaccharides r\ue9p\ue9t\ue9es poss\ue9dant la structure suivante: ->2)-b-D-Glcp-(1->2)-b-DFucp3NBu-( 1->6)-a-D-GlcpNAc-(1->4)-a-D-GalpNAc-(1->3)-b-D-GlcpNAc-(1->, o\uf9 Bu est le (R)-3-hydroxybutyramido.Peer reviewed: YesNRC publication: Ye

Characterization of the antigenic lipopolysaccharide O chain and the capsular polysaccharide produced by Actinobacillus pleuropneumoniae serotype 13

NRC publication: Ye

The structure of the polysaccharide O-chain of the LPS from Acinetobacter baumannii strain ATCC 17961

The gram-negative bacterium Acinetobacter baumannii strain ATCC17961 has been used by several laboratories in mouse models of respiratory A. baumannii infection, and a study of the role of its lipopolysaccharide in the pathogenicity is of interest. The structure of the O-deacylated polysaccharide O-chain component of its LPS has been determined by 2D NMR spectroscopy and mass spectrometry methods, and by the structural identification of oligosaccharides obtained by sequential application of the Smith degradation of the O-antigen. The O-chain was determined to be a polymer of a branched pentasaccharide repeating unit composed of 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid, 2-acetamido-2-deoxy- D-glucose, 2-acetamido-2-deoxy-D-galactose, D-glucose, and D-galactosePeer reviewed: YesNRC publication: Ye

The structural characterization of the O-polysaccharide antigen of the lipopolysaccharide of Escherichia coli serotype O118 and its relation to the O-antigens of Escherichia coli O151 and Salmonella enterica O47

Mild acid hydrolysis of the lipopolysaccharide produced by Escherichia coli O118:H16 standard strain (NRCC 6613) afforded an O-polysaccharide (O-PS) composed of D-galactose, 2-acetamidoylamino-2,6- dideoxy-L-galactose, 2-acetamido-2-deoxy-D-glucose, ribitol, and phosphate (1:1:1:1:1). From DOC-PAGE, sugar and methylation analyses, one- and two-dimensional NMR spectroscopy, capillary electrophoresismass spectrometry, hydrolysis, and sequential Smith-type periodate oxidation studies, the O-PS was determined to be an unbranched linear polymer having the structure: [6)-\u3b1-D-Galp-(1->3)-\u3b1-L-FucpNAm-(1->3)-\u3b2-D-GlcpNAc-(1->3)-Rib-ol-5-P-(O->]n The structure of the O-PS is consistent with the reported DNA data on the O-antigen gene-cluster of E. coli O118 and interestingly, the O-PS is similar to the structures of the O-antigens of Salmonella enterica O47 and E. coli O151:H10 reference strain 880-67, as predicted from the results of DNA sequencing of their respective O-antigen gene-clusters.Peer reviewed: YesNRC publication: Ye

Structure of the antigenic repeating pentasaccharide unit of the LPS O-polysaccharide of Cronobacter sakazakii implicated in the Tennessee outbreak

Strains of the Gram-negative bacterium Cronobacter (Enterobacter) sakazakii have been identified as emerging opportunistic pathogens that can cause enterocolitis, bacteraemia, meningitis, and brain abscess, and have been particularly associated with meningitis in neonates where infant-milk formulae has been epidemiologically linked to the disease. A study of the lipopolysaccharides produced by clinical isolates using chemical, 2D \ub9H and \ub9\ub3C NMR, and MS methods revealed that the O-polysaccharide produced by C. sakazakii (3290), a clinical strain from the Tennessee outbreak, was a branched polymer of repeating pentasaccharide units composed of 2-acetamido-2-deoxy-D-galactose, 3-(N-acetyl-L-alanylamido)-3-deoxy-D-quinovose, D-glucuronic acid, and D-glucose present in the molar ratio 1:1:1:2. The O-PS structure provides a unique specific structurally defined marker for the clinical tracking of this pathogen.Des souches de la bact\ue9rie Gram-n\ue9gative Cronobacter (Enterobacter) sakazakii ont \ue9t\ue9 identifi\ue9es comme pathog\ue8nes opportunistes en \ue9mergence qui peuvent causer l\u2019ent\ue9rocolite, une bact\ue9ri\ue9mie, la m\ue9ningite et des abc\ue8s c\ue9r\ue9braux, et elles ont \ue9t\ue9 particuli\ue8rement associ\ue9es \ue0 la m\ue9ningite chez le nouveau-n\ue9 o\uf9 des formules de lait pour b\ue9b\ue9s ont \ue9t\ue9 li\ue9es \ue9pid\ue9miologiquement \ue0 la maladie. Une \ue9tude des polysaccharides produits par des isolats cliniques r\ue9alis\ue9e \ue0 l\u2019aide de m\ue9thodes chimiques, de RMN du 2D\ub9 et du C\ub9\ub3 et de SM ont r\ue9v\ue9l\ue9 que le O-polysaccharide produit par C. sakasakii (3290), une souche clinique a` l\u2019origine d\u2019une \ue9pid\ue9mie au Tennessee, est un polym\ue8re branch\ue9 d\u2019unit\ue9s r\ue9p\ue9t\ue9es d\u2019un pentasaccharide compos\ue9 de 2-ac\ue9tamido-2-d\ue9soxy-D-galactose, de 3-(N-ac\ue9tyl-L-alanylamido)-3-d\ue9soxy-D-quinovose, d\u2019acide D-glucuronique et de D-glucose pr\ue9sents dans un ratio molaire 1:1:1:2. La structure de l\u2019O-PS constitue un marqueur unique et sp\ue9cifique, structurellement d\ue9fini, pour retracer cliniquement ce pathog\ue8ne.Peer reviewed: YesNRC publication: Ye

The structure of the O-antigen in the endotoxin of the emerging food pathogen Cronobacter (Enterobacter) muytjensii strain 3270

Strains of the Gram-negative bacterium Cronobacter (formerly known as Enterobacter) sakazakii have been identified as emerging opportunistic pathogens that can cause enterocolitis, bacteraemia, meningitis, and brain abscess, and they have been particularly associated with meningitis in neonates where infant milk formulae have been epidemiologically linked to the disease. A study of the lipopolysaccharides produced by clinical isolates using chemical, 2D \ub9H and \ub9\ub3C NMR, and MS methods revealed that the O-polysaccharide produced by Cronobacter muytjensii strain 3270, isolated from powdered infant formula from Denmark, was a linear unbranched polymer of a repeating pentasaccharide unit composed of 2-acetamido-2-deoxy-D-galactose (D-GalNAc), 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), 3-acetamido- 3-deoxy-D-quinovose (D-Qui3NAc), L-rhamnose (L-Rha), and D-glucuronic acid (D-GlcA) in equimolar ratio, and has the structure \u21923)-\u3b1-D-GalpNAc-(1\u21924)-\u3b1-D-Quip3NAc-(1\u21923)-\u3b1-L-Rhap-(1\u21926)-\u3b1-D-GlcpNAc-(1\u21924)-\u3b2-D-GlcpA-(1\u2192 The specific structural characteristics of the O-polysaccharides of C. muytjensii may be of value in the identification and tracking of the bacterial pathogen.Peer reviewed: YesNRC publication: Ye

Characterization of the O-antigen in the lipopolysaccharide of Cronobacter (Enterobacter) malonaticus 3267

Cronobacter malonaticus, a Gram-negative bacterium previously known as Enterobacter sakazakii, is an opportunistic pathogen known to cause serious infection in infants and neonates. To provide aid for the serological and chemical identification of clinical, environmental, or food isolates of this emerging pathogen, the characterization of the lipopolysaccharide (LPS) O-polysaccharide (O-PS) antigens of Cronobacter spp. is being undertaken. The structural analysis of the O-PS, obtained by hydrazinolysis of the lipopolysaccharide produced by Cronobacter malonaticus HPB 3267, was investigated by composition, methylation, and two-dimensional high-resolution nuclear magnetic resonance methods, and was found to be a polymer of a branched pentasaccharide unit. This unit is composed of D-glucose (D-Glc), D-galactose (D-Gal), 2-amino-2-deoxy-D-glucose (D-GlcN), 2-amino-2-deoxy-D-galactose (D-GalN) and 3-deoxy-D-manno-oct-2- ulosonic acid (Kdo) residues (1: 1: 1: 1: 1), forms the repeating oligosaccharide in the O-PS antigenCronobacter malonaticus, une bact\ue9rie \ue0 Gram n\ue9gatif, connue pr\ue9c\ue9demment sous le nom de Enterobacter sakazakii, est un pathog\ue8ne opportuniste qui cause des infections graves chez le b\ue9b\ue9 et le nouveau-n\ue9. Afin d\u2019aider \ue0 l\u2019identification s\ue9rologique et chimique des isolats cliniques, environnementaux ou alimentaires de ce pathog\ue8ne en \ue9mergence, la caract\ue9risation du LPS O-polysaccharide antig\ue9nique de Cronobacter spp. a \ue9t\ue9 entreprise. La structure de l\u2019O-PS obtenu apr\ue8s hydrazinolyse du lipopolysaccharide produit par la souche Cronobacter malonaticus HPB 3267 a \ue9t\ue9 examine\ue9 par composition, m\ue9thylation et r\ue9sonance magn\ue9tique nucl\ue9aire 2D \ue0 haute r\ue9solution. Il consiste en un polym\ue8re de penta-saccharides branch\ue9s. L\u2019unit\ue9 est compos\ue9e de D-glucose (D-Glc), de D-galactose (D-Gal), de 2-amino-2-d\ue9oxy-D-glucose (D-GlcN), de 2-amino-2-d\ue9oxy-D-galactose (D-GalN) et d\u2019acide 3-d\ue9oxy-D-manno-oct-2-ulosonique (Kdo) (1 :1 :1 :1 :1), et elle forme l\u2019oligosaccharide r\ue9p\ue9t\ue9 constituant l\u2019O-PSPeer reviewed: YesNRC publication: Ye

The structure of the O-antigen of Cronobacter sakazakii HPB 2855 isolate involved in a neonatal infection

Strains of Cronobacter sakazakii (previously known as Enterobacter sakazakii) are medically recognized important Gram-negative bacterial pathogens that cause enterocolitis, septicemia, and meningitis, with a high mortality rate in neonates. The structure of their O-antigens, that form part of their somatic lipopolysaccharide (LPS) components, is of interest for their chemical and serological identification and their relationship to virulence. The O-polysaccharide (O-PS) of C. sakazakii HPB 2855 (SK 81), a strain isolated from an infant at the Hospital for Sick Children in Toronto in 1981, was shown to be a polymer of a partially O-acetylated-repeating hexasaccharide unit composed of D-glucose, D-galacturonic acid, 2-acetamido- 2-deoxy-D-galactose, and L-rhamnose (1:1:1:3). From composition and methylation analysis, and the application of 1D and 2D 1H and 13C NMR spectroscopy, the O-PS was determined to be a polymer of a repeating oligosaccharide unit having the structurePeer reviewed: YesNRC publication: Ye