Phylogenetic Analysis of Seven WRKY Genes across the Palm Subtribe Attaleinae (Arecaceae) Identifies Syagrus as Sister Group of the Coconut

BACKGROUND:The Cocoseae is one of 13 tribes of Arecaceae subfam. Arecoideae, and contains a number of palms with significant economic importance, including the monotypic and pantropical Cocos nucifera L., the coconut, the origins of which have been one of the "abominable mysteries" of palm systematics for decades. Previous studies with predominantly plastid genes weakly supported American ancestry for the coconut but ambiguous sister relationships. In this paper, we use multiple single copy nuclear loci to address the phylogeny of the Cocoseae subtribe Attaleinae, and resolve the closest extant relative of the coconut. METHODOLOGY/PRINCIPAL FINDINGS:We present the results of combined analysis of DNA sequences of seven WRKY transcription factor loci across 72 samples of Arecaceae tribe Cocoseae subtribe Attaleinae, representing all genera classified within the subtribe, and three outgroup taxa with maximum parsimony, maximum likelihood, and Bayesian approaches, producing highly congruent and well-resolved trees that robustly identify the genus Syagrus as sister to Cocos and resolve novel and well-supported relationships among the other genera of the Attaleinae. We also address incongruence among the gene trees with gene tree reconciliation analysis, and assign estimated ages to the nodes of our tree. CONCLUSIONS/SIGNIFICANCE:This study represents the as yet most extensive phylogenetic analyses of Cocoseae subtribe Attaleinae. We present a well-resolved and supported phylogeny of the subtribe that robustly indicates a sister relationship between Cocos and Syagrus. This is not only of biogeographic interest, but will also open fruitful avenues of inquiry regarding evolution of functional genes useful for crop improvement. Establishment of two major clades of American Attaleinae occurred in the Oligocene (ca. 37 MYBP) in Eastern Brazil. The divergence of Cocos from Syagrus is estimated at 35 MYBP. The biogeographic and morphological congruence that we see for clades resolved in the Attaleinae suggests that WRKY loci are informative markers for investigating the phylogenetic relationships of the palm family

Passiflora eglandulosa J.M. MacDougal

Guatemala, San Marcos, Aldea Fraternidad, Near Aldea Fraternidad, between San Rafael Pie de la Cuesta and Palo Gordo, west facing slope of the Sierra Madre Mountains. LAT (0) N; LONG (0) W. ALT (1800)

Passiflora quetzal J.M. MacDougal

Guatemala San Marcos Aldea Fraternidad Near Aldea Fraternidad, between San Rafael Pie de La Cuesta and Palo Gordo, west facing slope of the Sierra Madre Mountain. LAT (0)N; LONG(0)W. ALT (1800

Middle cerebral artery blood velocity is reduced with hyperthermia during prolonged exercise in humans

In the present study we examined the effect of hyperthermia on the middle cerebral artery mean blood velocity (MCA Vmean) during prolonged exercise. We predicted that the cerebral circulation would be impaired when hyperthermia is present during exercise and assumed that this could be observed as a reduced MCA Vmean.Eight endurance trained men (maximum oxygen uptake (V̇O2,max) 70 ± 1 ml min−1 kg−1 (mean ±s.e.m.)) performed two exercise trials at 57 % of V̇O2,max on a cycle ergometer in a hot (40 °C; hyperthermic trial) and in a thermoneutral environment (18 °C; control trial). In the hyperthermic trial, the oesophageal temperature increased throughout the exercise period reaching a peak value of 40.0 ± 0.1 °C at exhaustion after 53 ± 4 min of exercise. In the control trial, exercise was maintained for 1 h without any signs of fatigue and with core temperature stabilised at 37.8 ± 0.1 °C after ≈15 min of exercise.Concomitant with the development of hyperthermia, MCA Vmean declined by 26 ± 3 % from 73 ± 4 cm s−1 at the beginning of exercise to 54 ± 4 cm s−1 at exhaustion (P < 0.001). In contrast, MCA Vmean remained unchanged at 70-72 cm s−1 throughout the 1 h control trial.When individually determined regression lines for MCA Vmean and arterial carbon dioxide pressure (Pa,CO2) obtained during preliminary exercise tests were used to account for the differences in Pa,CO2 between the hyperthermic and control trial, it appeared that more than half of the reduction in MCA Vmean (56 ± 8 %) was related to a hyperventilation-induced drop in Pa,CO2. Declining cardiac output and arterial blood pressure accounted for the remaining part of the hyperthermia-induced reduction in MCA Vmean.The present results demonstrate that the development of hyperthermia during prolonged exercise is associated with a marked reduction in MCA Vmean