Incretins and bone: friend or foe?

To adapt to its various functions, the bone tissue is remodeled permanently and is under the influence of hormonal, local, mechanical and nervous signals. Among them, a role for gut hormones in controlling bone mass and quality has emerged in the recent years. The aim of this review is to provide the reader with a summary of recent developments in the interaction between incretin hormones and bone physiology

Interplay between bone and incretin hormones: A review

Bone is a tissue with multiple functions that is built from the molecular to anatomical levels to resist and adapt to mechanical strains. Among all the factors that might control the bone organization, a role for several gut hormones called "incretins" has been suspected. The present review summarizes the current evidences on the effects of glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) in bone physiology

Novel skeletal effects of glucagon-like peptide-1 (GLP-1) receptor agonists

Type 2 diabetes mellitus (T2DM) leads to bone fragility and predisposes to increased risk of fracture, poor bone healing and other skeletal complications. In addition, some anti-diabetic therapies for T2DM can have notable detrimental skeletal effects. Thus, an appropriate therapeutic strategy for T2DM should not only be effective in re-establishing good glycaemic control but also in minimising skeletal complications. There is increasing evidence that glucagon-like peptide-1 receptor agonists (GLP-1RAs), now greatly prescribed for the treatment of T2DM, have beneficial skeletal effects although the underlying mechanisms are not completely understood. This review provides an overview of the direct and indirect effects of GLP-1RAs on bone physiology, focusing on bone quality and novel mechanisms of action on the vasculature and hormonal regulation. The overall experimental studies indicate significant positive skeletal effects of GLP-1RAs on bone quality and strength although their mechanisms of actions may differ according to various GLP-1RAs and clinical studies supporting their bone protective effects are still lacking. The possibility that GLP-1RAs could improve blood supply to bone, which is essential for skeletal health, is of major interest and suggests that GLP-1 anti-diabetic therapy could benefit the rising number of elderly T2DM patients with osteoporosis and high fracture risk

Présentation des plateformes SCIAM et IRM-PRIMEX

Présentation des plateformes SCIAM et IRM-PRIMEX Guillaume Mabilleau et Florence Franconi Université d’Angers, Institut de Biologie en Santé, 49933 Angers, France Le service commun d’imageries et d’analyses microscopiques (SCIAM) et la plateforme d’Imagerie par Résonance Magnétique (IRM) préclinique – PRIMEX sont deux services communs de la SFR ICAT 4208 de l’Université d’Angers. Le SCIAM dispose d’un ensemble dispose d’un microtomographe à rayons X (Bruker microCT 1076) permettant l’acquisition in vivo à des résolutions de 9, 18 et 36 µm et d’un irradiateur à rayons X (Faxitron CP-160). La plateforme PRIMEX dispose d’un imageur Biospec 70/20 Avance III (Bruker Biospin) opérant à un champ magnétique de 7 Tesla. Ces équipements sont particulièrement adaptés pour l’imagerie de la souris ou du rat. Un tour d’horizon du potentiel de ces équipements en préclinique sera présenté. Ces équipements permettent des explorations in vivo chez le petit animal pour des applications telles que la caractérisation anatomique ou fonctionnelle de modèle animaux en oncologie, neurologie, cardiologie… ou l’évaluation de l’efficacité de nouveaux traitements thérapeutiques. Des applications dans d’autres domaines sont aussi possibles (végétal, agroalimentaire, matériaux, ex vivo…). Ces deux plateformes sont ouvertes aux partenaires institutionnels et industriels. Elles proposent un accompagnement scientifique et technique à la conception de projets ainsi que des formations dans le domaine de l’IRM préclinique

Number of Circulating CD14-Positive Cells and the Serum Levels of TNF-α Are Raised in Acute Charcot Foot

International audienc

Location Estimation in a Smart Home: System Implementation and Evaluation Using Experimental Data

In the context of a constantly increasing aging population with cognitive deficiencies, insuring the autonomy of the elders at home becomes a priority. The DOMUS laboratory is addressing this issue by conceiving a smart home which can both assist people and preserve their quality of life. Obviously, the ability to monitor properly the occupant's activities and thus provide the pertinent assistance depends highly on location information inside the smart home. This paper proposes a solution to localize the occupant thanks to Bayesian filtering and a set of anonymous sensors disseminated throughout the house. The localization system is designed for a single person inside the house. It could however be used in conjunction with other localization systems in case more people are present. Our solution is functional in real conditions. We conceived an experiment to estimate precisely its accuracy and evaluate its robustness. The experiment consists of a scenario of daily routine meant to maximize the occupant's motion in meaningful activities. It was performed by 14 subjects, one subject at a time. The results are satisfactory: the system's accuracy exceeds 85% and is independent of the occupant's profile. The system works in real time and behaves well in presence of noise

Hypodynamia Alters Bone Quality and Trabecular Microarchitecture

Disuse induces a rapid bone loss in humans and animals; hypodynamia/sedentarity is now recognized as a risk factor for osteoporosis. Hypodynamia also decreases bone mass but its effects are largely unknown and only few animal models have been described. Hypodynamic chicken is recognized as a suitable model of bone loss but the effects on the quality have not been fully explored. We have used ten chickens bred in a large enclosure (FREE group); ten others were confined in small cages with little space to move around (HYPO group). They were sacrificed at 53 days and femurs were evaluated by microcomputed tomography (microCT) and nanoindentation. Sections (4 µm thick) were analyzed by Fourier Transform InfraRed Microspectroscopy (FTIR) to see the effects on mineralization and collagen and quantitative backscattered electron imaging (qBEI) to image the mineral of the bone matrix. Trabecular bone volume and microarchitecture were significantly altered in the HYPO group. FTIR showed a significant reduction of the mineral-to-matrix ratio in the HYPO group associated with an increase in the carbonate content and an increase in crystallinity (calculated as the area ratio of subbands located at 1020 and 1030 cm(-1)) indicating a poor quality of the mineral. Collagen maturity (calculated as the area ratio of subbands located at 1660 and 1690 cm(-1)) was significantly reduced in the HYPO group. Reduced biomechanical properties were observed at the tissue level. Confined chicken represents a new model for the study of hypodynamia because bone changes are not created by a surgical lesion or a traumatic method. Animals have a reduced bone mass and present with an altered bone matrix quality which is less mineralized and whose collagen contains less crosslinks than in control chicken

Abrasion of 6 dentifrices measured by vertical scanning interference microscopy

OBJECTIVES: The abrasion of dentifrices is well recognized to eliminate the dental plaque. The aims of this study were to characterize the abrasive powders of 6 dentifrices (3 toothpastes and 3 toothpowders) and to measure the abrasion on a test surface by Vertical Scanning Interference microscopy (VSI). MATERIAL AND METHODS: Bright field and polarization microscopy were used to identify the abrasive particles on the crude dentifrices and after prolonged washes. Scanning electron microscopy and microanalysis characterized the shape and nature of the particles. Standardized and polished blocks of poly(methylmethacrylate) were brushed with a commercial electric toothbrush with the dentifrices. VSI quantified the mean roughness (Ra) and illustrated in 3D the abraded areas. RESULTS: Toothpastes induced a limited abrasion. Toothpowders induced a significantly higher roughness linked to the size of the abrasive particles. One powder (Gencix® produced a high abrasion when used with a standard testing weight. However, the powder is based on pumice particles covered by a plant homogenate that readily dissolves in water. When used in the same volume, or after dispersion in water, Ra was markedly reduced. CONCLUSION: Light and electron microscopy characterize the abrasive particles and VSI is a new tool allowing the analysis of large surface of abraded materials

Aluminum and bone: Review of new clinical circumstances associated with Al(3+) deposition in the calcified matrix of bone

Several decades ago, aluminum encephalopathy associated with osteomalacia has been recognized as the major complication of chronic renal failure in dialyzed patients. Removal of aluminum from the dialysate has led to a disappearance of the disease. However, aluminum deposit occurs in the hydroxyapatite of the bone matrix in some clinical circumstances that are presented in this review. We have encountered aluminum in bone in patients with an increased intestinal permeability (coeliac disease), or in the case of prolonged administration of aluminum anti-acid drugs. A colocalisation of aluminum with iron was also noted in cases of hemochromatosis and sickle cell anemia. Aluminium was also identified in a series of patients with exostosis, a frequent benign bone tumor. Corrosion of prosthetic implants composed of grade V titanium (TA6V is an alloy containing 6% aluminum and 4% vanadium) was also observed in a series of hip or knee revisions. Aluminum can be identified in undecalcified bone matrix stained by solochrome azurine, a highly specific stain allowing the detection of 0.03 atomic %. Colocalization of aluminum and iron does not seem to be the fruit of chance but the cellular and molecular mechanisms are still poorly understood. Histochemistry is superior to spectroscopic analyses (EDS and WDS in scanning electron microscopy)