The diploid genome sequence of an Asian individual

Here we present the first diploid genome sequence of an Asian individual. The genome was sequenced to 36-fold average coverage using massively parallel sequencing technology. We aligned the short reads onto the NCBI human reference genome to 99.97% coverage, and guided by the reference genome, we used uniquely mapped reads to assemble a high-quality consensus sequence for 92% of the Asian individual's genome. We identified approximately 3 million single-nucleotide polymorphisms (SNPs) inside this region, of which 13.6% were not in the dbSNP database. Genotyping analysis showed that SNP identification had high accuracy and consistency, indicating the high sequence quality of this assembly. We also carried out heterozygote phasing and haplotype prediction against HapMap CHB and JPT haplotypes (Chinese and Japanese, respectively), sequence comparison with the two available individual genomes (J. D. Watson and J. C. Venter), and structural variation identification. These variations were considered for their potential biological impact. Our sequence data and analyses demonstrate the potential usefulness of next-generation sequencing technologies for personal genomics

Genome Editing and Its Applications in Model Organisms

Technological advances are important for innovative biological research. Development of molecular tools for DNA manipulation, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), has revolutionized genome editing. These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases. In the last few years, substantial progress has been made in CRISPR/Cas technology, including technical improvements and wide application in many model systems. This review describes recent advancements in genome editing with a particular focus on CRISPR/Cas, covering the underlying principles, technological optimization, and its application in zebrafish and other model organisms, disease modeling, and gene therapy used for personalized medicine

Vision-Based Formation Control for an Outdoor UAV Swarm With Hierarchical Architecture

Formation control of a UAV swarm is challenging in outdoor GNSS-denied environments due to the difficulties in accomplishing relative positioning among the UAVs. This study proposes a vision-based formation control strategy that could be implemented in the absence of an external positioning system. The hierarchical architecture has been constructed for the UAV swarm using the modified leader-follower strategy. The leader UAV derives and broadcasts the locations of the follower UAVs, while the follower UAVs calculate their control inputs to achieve the desired swarm formation. The vision-based localization of the UAVs is accomplished using state-of-the-art deep learning algorithms like YOLOv7 and DeepSORT. The RflySim-based simulation has been conducted to verify the feasibility of the conceptualization, and the validation has been made with a real flight test using a swarm comprised of five quadrotors. Results show the robustness of the vision-based UAV positioning framework with a localization error within 0.3 m. Moreover, the formation control without the GNSS is achieved with a monocular camera and the entry-level AI platforms implemented onboard, which could be promoted to UAV swarm applications for broader scenarios

Effect of highly branched hyphal morphology on the enhanced production of cellulase in Trichoderma reesei DES-15

The morphology of Trichoderma reesei is a vitally important factor for cellulase productivity. This study investigated the effect of hyphal morphology on cellulase production in the hyper-cellulolytic mutant, T. reesei DES-15. With a distinct morphology, T. reesei DES-15 was obtained through Diethyl sulfite (DES) mutagenesis. The hyphal morphology of DES-15 batch-cultured in a 5-L fermentor was significantly shorter and more branched than the parental strain RUT C30. The cellulase production of DES-15 during batch fermentation was 66 % greater than that of RUT C30 when cultured the same conditions. DES-15 secreted nearly 50 % more protein than RUT C30. The gene expression level of a set of genes (cla4, spa2, ras2, ras1, rhoA, cdc42, and racA) known to be involved in hyphae growth and hyphal branching was measured by quantitative real-time PCR. The transcriptional analysis of these genes demonstrated that a decrease in gene expressions might contribute to the increased hyphal branching seen in DES-15. These results indicated that the highly branching hyphae in DES-15 resulted in increased cellulase production, suggesting that DES-15 may be a good candidate for use in the large-scale production of cellulase

The Influence of Meteorological Variables on Reference Evapotranspiration Based on the FAO P-M Model—A Case Study of the Taohe River Basin, NW China

To explore the mechanisms driving variation in ET0 (reference evapotranspiration) in an arid inland region of Northwest China, daily meteorological data from 1960 to 2019 from 19 meteorological stations in the Taohe River basin and its surrounding areas were used to analyze the temporal and spatial distributions of ET0 and meteorological variables. Various qualitative and quantitative analysis methods were used to reveal the correlation between ET0 and meteorological variables. The degree of sensitivity of ET0 variations to meteorological variables and the contribution from each meteorological variable were clarified, and the mechanisms driving variation in ET0 were fully revealed. These are the results: (1) ET0 in the Taohe River basin presented a significant upward trend with a linear change rate of 0.93 mm/a, and a sudden change occurred in 1994. The spatial variation in ET0 ranged from 779.8 to 927.6 mm/a, with low values in the upper and middle reaches and high values in the lower reaches. The ET0 at 14 stations (73.68% of the total) was significantly increased (p p > 0.05). (2) RH, Rn, and u2 did not change significantly, while Tmax and Tmin showed a significant upward trend. (3) Rn is a meteorological variable closely related to variations in ET0, and is the most sensitive variable for variations in ET0, followed by Tmax and u2. (4) Tmax is the meteorological variable that contributes most to the variation in ET0 (30.98%), followed by Tmin (29.11%), u2 (6.57%), Rn (2.22%), and RH (0.05%). The research results provide a scientific basis for the rational and efficient utilization of water resources and the maintenance of ecosystem health

Detector location selection based on VIP analysis in near-infrared detection of dural hematoma

Detection of dural hematoma based on multi-channel near-infrared differential absorbance has the advantages of rapid and non-invasive detection. The location and number of detectors around the light source are critical for reducing the pathological characteristics of the prediction model on dural hematoma degree. Therefore, rational selection of detector numbers and their distances from the light source is very important. In this paper, a detector position screening method based on Variable Importance in the Projection (VIP) analysis is proposed. A preliminary modeling based on Partial Least Squares method (PLS) for the prediction of dural position μa was established using light absorbance information from 30 detectors located 2.0–5.0 cm from the light source with a 0.1 cm interval. The mean relative error (MRE) of the dural position μa prediction model was 4.08%. After VIP analysis, the number of detectors was reduced from 30 to 4 and the MRE of the dural position μa prediction was reduced from 4.08% to 2.06% after the reduction in detector numbers. The prediction model after VIP detector screening still showed good prediction of the epidural position μa. This study provided a new approach and important reference on the selection of detector location in near-infrared dural hematoma detection. Keywords: Detector location screening, Epidural hematoma detection, Variable importance in the projectio