Breeding in bambara groundnut (Vigna subterranea (L.) Verdc.): strategic considerations

Bambara groundnut (Vigna subterranea (L.) Verdc.) has a large number of landraces throughout Africa where small-scale farmers have preserved its genetic diversity on-farm. To date, the full genetic diversity of the crop remains largely unexploited. Until recently bambara groundnut never received any appreciable research effort, especially for its genetic improvement. Until then, only selection breeding was practised in which existing landraces were evaluated and their seeds multiplied. However, no new combinations resulting from hybridisation had ever been produced. Recently, collaborative research efforts involving partners from Africa and Europe have produced the first crosses of bambara groundnut. The creation of these crosses is a significant scientific and practical achievement and opens up the possibility of breeding true varieties of this crop. This paper shows how different strategies have been combined to establish the basis of a strategic breeding programme in bambara groundnut. The paper also illustrates the use of landraces in the bambara groundnut breeding programme, as an example of the contribution that landraces can make to increasing productivity in marginal environments and the conservation of a crop's genetic resources on-farm.African Journal of Biotechnology Vol. 4 (6), pp. 463-471, 200

21 tapaa tehostaa korkeakouluopintoja

Tämä julkaisu avaa 21 tapaa tehostaa korkeakouluopintoja. Kymmenen ammattikorkeakoulun pedagogia ja kehittäjää tuovat näkyviin konkreettisia toimintatapoja ja pedagogisia ratkaisuja, joilla tutkinto-opiskelua voidaan ketteröittää. Kantavina teemoina ovat työn ja opintojen kietoutuminen toisiinsa sekä rikastuttava keskinäinen vuorovaikutus. Toimivia ratkaisuja tunnistetaan oppimisympäristöistä, osaamisperustaisesta ohjauksesta ja arvioinnista sekä autenttisen työn ja opintojen reaaliaikaisesta yhdistämisestä. Kirjassa pohditaan myös, millä tavalla osaamisen kehittäminen voi edistää tuottavuutta korkeakoulussa. Kirja on tarkoitettu korkeakoulussa toimiville pedagogeille ja tutkijakehittäjille sekä korkeakoulun johdolle. Toivomme kirjan löytävän myös kaikki ne muut tahot ja henkilöt, jotka kehittävät maamme korkeakoulutoiminnan ja työn välistä integraatiota. Olemme osaamisen ajassa ja korkeakoulujen on löydettävä siinä luonteva paikkansa kehittävänä toimijana

Learning and livelihoods: The experience of the FSIPM project in southern Malawi

Do resource-poor farmers in sub-Saharan Africa face severe crop losses from pests and diseases? What are the different ways that rural households earn a living, and who are the poor? How does social organization shape the way that new technology is used and shared? How can we encourage farmers to participate in the design and evaluation of field experiments, yet still obtain results that are statistically valid? How can we create 'learning projects' that can change direction in response to the needs of their clients and what they find on the ground? These were some of the questions that challenged the Farming Systems Integrated Pest Management Project during four years of intensive fieldwork with resource-poor farmers in the Blantyre Shire Highlands, southern Malawi. In searching for answers, the project drew on expertise from pest management, agronomy, economics and anthropology. Learning and Livelihoods: The Experience of the FSIPM Project in Southern Malawi reflects on the project's experience, reviews the major lessons learned, and outlines an agenda for a follow-up project that addresses smallholders' needs for food security, cash income and information about new technology. The book will appeal not only to those interested in Malawi but also to those concerned with the wider issues raised by developing technology with resource poor farmers

Efficacy of WHO recommendation for continued breastfeeding and maternal cART for prevention of perinatal and postnatal HIV transmission in Zambia

Introduction: To prevent mother-to-child transmission (MTCT) of HIV in developing countries, new World Health Organization (WHO) guidelines recommend maternal combination antiretroviral therapy (cART) during pregnancy, throughout breastfeeding for 1 year and then cessation of breastfeeding (COB). The efficacy of this approach during the first six months of exclusive breastfeeding has been demonstrated, but the efficacy of this approach beyond six months is not well documented. Methods: A prospective observational cohort study of 279 HIV-positive mothers was started on zidovudine/3TC and lopinavir/ritonavir tablets between 14 and 30 weeks gestation and continued indefinitely thereafter. Women were encouraged to exclusively breastfeed for six months, complementary feed for the next six months and then cease breastfeeding between 12 and 13 months. Infants were followed for transmission to 18 months and for survival to 24 months. Text message reminders and stipends for food and transport were utilized to encourage adherence and follow-up. Results: Total MTCT was 9 of 219 live born infants (4.1%; confidence interval (CI) 2.2-7.6%). All breastfeeding transmissions that could be timed (5/5) occurred after six months of age. All mothers who transmitted after six months had a six-month plasma viral load \u3e1,000 copies/ml (p\u3c0.001). Poor adherence to cART as noted by missed dispensary visits was associated with transmission (p=0.04). Infant mortality was lower after six months of age than during the first six months of life (p=0.02). The cumulative rate of infant HIV infection or death at 18 months was 29/226 (12.8% 95 CI: 7.5-20.8%). Conclusions: Maternal cART may limit MTCT of HIV to the UNAIDS target of \u3c5% for eradication of paediatric HIV within the context of a clinical study, but poor adherence to cART and follow-up can limit the benefit. Continued breastfeeding can prevent the rise in infant mortality after six months seen in previous studies, which encouraged early COB

Chitosan-based hydrogels supplemented with gelatine and Link N enhance extracellular matrix deposition by encapsulated cells in a degenerative intervertebral disc environment

Injectable therapies for intervertebral disc (IVD) repair are gaining much interest. Recently, a chitosan (CH)-based injectable scaffold has been developed that has similar mechanical properties to human nucleus pulposus (NP) and provides a suitable environment for encapsulated NP cell survival and proteoglycan production. The hypothesis of the study was that the biological response of the encapsulated cells can be further increased by adding gelatine and Link N (LN, a naturally occurring peptide present in cartilage and IVD extracellular matrix), known to increase cell adhesion and proteoglycan production, respectively. The effect of gelatine on the mechanical properties of a CH hydrogel was evaluated through rheological and compressive mechanical tests. Production of proteoglycan [assessed as glycosaminoglycan (GAG)] by encapsulated NP cells was determined in the presence or absence of gelatine in normal or degenerative medium supplemented with LN. Normal and degenerative media replicate the healthy and degenerative disc environment, respectively. Gelatine slightly reduced the gelation rate of CH hydrogel but improved its final mechanical properties in compression. LN had a minimal effect in normal medium but induced significantly more GAG production in degenerative medium (p < 0.001, 4.7-fold superior to the control), reaching similar results to transforming growth factor (TGF)-β (used as a positive control). GAG production was further increased in CH-gelatine hydrogels, confirming an additive effect of LN and gelatine in a degenerative environment. The results supported the concept that CH-gelatine hydrogels supplemented with LN can help restore the function of the NP during the early stages of IVD degeneration

The Potential of N-Rich Plasma-Polymerized Ethylene (PPE:N) Films for Regulating the Phenotype of the Nucleus Pulposus

We recently developed a nitrogen-rich plasma-polymerized biomaterial, designated “PPE:N” (N-doped plasma-polymerized ethylene) that is capable of suppressing cellular hypertrophy while promoting type I collagen and aggrecan expression in mesenchymal stem cells from osteoarthritis patients. We then hypothesized that these surfaces would form an ideal substrate on which the nucleus pulposus (NP) phenotype would be maintained. Recent evidence using microarrays showed that in young rats, the relative mRNA levels of glypican-3 (GPC3) and pleiotrophin binding factor (PTN) were significantly higher in nucleus pulposus (NP) compared to annulus fibrosus (AF) and articular cartilage. Furthermore, vimentin (VIM) mRNA levels were higher in NP versus articular cartilage. In contrast, the levels of expression of cartilage oligomeric matrix protein (COMP) and matrix gla protein precursor (MGP) were lower in NP compared to articular cartilage. The objective of this study was to compare the expression profiles of these genes in NP cells from fetal bovine lumbar discs when cultured on either commercial polystyrene (PS) tissue culture dishes or on PPE:N with time. We found that the expression of these genes varies with the concentration of N ([N]). More specifically, the expression of several genes of NP was sensitive to [N], with a decrease of GPC3, VIM, PTN, and MGP in function of decreasing [N]. The expression of aggrecan, collagen type I, and collagen type II was also studied: no significant differences were observed in the cells on different surfaces with different culture time. The results support the concept that PPE:N may be a suitable scaffold for the culture of NP cells. Further studies are however necessary to better understand their effects on cellular phenotypes

CCARDESA Agribusiness and Gender and Youth Responsive Food Systems

The Centre for Coordination of Agricultural Research and Development for Southern Africa (CCARDESA) is a SADC subsidiary mandated by Members States to coordinate regional cooperation in agricultural research and development. It is implementing the Comprehensive Africa Agriculture Development Programme EX Pillar 4 (CAADP-XP4) Programme on Gender. The CAADP-XP4 Programme is financed through the International Fund for Agricultural Development (IFAD) for four years, between 2019-2023. The CAADP-XP4 programme supports a science-led and climate-relevant agricultural transformation in Africa. It aims at strengthening the capacities of CCARDESA to deliver on their Agricultural Research for Development (AR4D) mandate and to support African countries collectively implementing relevant programmes of the Comprehensive Africa Agriculture Development Programme (CAADP) through inclusive regional and international partnerships; production and exchange of climate-relevant agricultural knowledge; effective communication, monitoring and evaluation; promotion of systemic and effective use of science, knowledge and innovation; and representation of the Sub Regional and National Organizations at Continental level. The programme seeks to achieve outputs, and one of them is to build the capacity of women-led and youth-led organizations to participate in strategic events and global climate issues. This entails enhancing partnerships among the multi-stakeholders across sectors and strengthening capacities of the implementing countries on gender strengthening/capacity to realize the outputs of the programme. One of the roles of the CAADP-XP4 programme is to strengthen and support women and youth in agribusiness for improved livelihood and increased income. As part of accomplishing this key activity, CCARDESA, in collaboration with AFAAS, ASARECA, CORAF and FARA, facilitated a gender-focused side event titled ‘The CCARDESA Fostering Agribusiness and Gender/YouthResponsive Food Systems’ side event at the Science Week in Durba

Building adaptive capacity to cope with increasing vulnerability due to climatic change in Africa – A new approach

The world community faces many risks from climate change, with most scenarios indicating higher temperatures and more erratic rainfall in Africa. Predictions for southern Africa suggest a general decrease in total seasonal rainfall, accompanied by more frequent in-season dry spells that will significantly impact crop and livestock production, and hence economic growth in the region. The hardest hit will be the rural poor in the drier areas, where crop failure due to drought is already common and chronic food emergencies afflict the region in most years. Lessons can be learnt on how the rural poor currently cope with the vagaries of climate and these can be used to help them adapt their current production systems to the future threats of further climate change. But this assumes the institutions that work towards the economic empowerment of the rural poor have the requisite skills to understand their current coping strategies and how adaptation can be facilitated. A new initiative led by Midlands State University and the Zambian Meteorological Office proposes that improving the ability of institutions that train the ‘Future Change Agents’, who will subsequently support smallholder communities in adapting their agricultural practices to current climate variability, is the first step in building adaptive capacity to cope with future climate change. The capacity of African scientists, regional organizations and decision-makers in dealing with the issues of climate change and adaptation will be enhanced on a continuing basis, and the impacts of their agricultural development programs improve

Effect of HIV self-testing on the number of sexual partners among female sex workers in Zambia: A randomized controlled trial

Objectives: To assess the effect of two health system approaches to distribute HIV selftests on the number of female sex workers’ client and nonclient sexual partners. Design: Cluster randomized controlled trial. Methods: Peer educators recruited 965 participants. Peer educator–participant groups were randomized 1 : 1 : 1 to one of three arms: delivery of HIV self-tests directly from a peer educator, free facility-based delivery of HIV self-tests in exchange for coupons, or referral to standard-of-care HIV testing. Participants in all three arms completed four peer educator intervention sessions, which included counseling and condom distribution. Participants were asked the average number of client partners they had per night at baseline, 1 and 4 months, and the number of nonclient partners they had in the past 12 months (at baseline) and in the past month (at 1 month and 4 months). Results: At 4 months, participants reported significantly fewer clients per night in the direct delivery arm (mean difference 0.78 clients, 95% CI 1.28 to 0.28, P ¼ 0.002) and the coupon arm (0.71, 95% CI 1.21 to 0.21, P ¼ 0.005) compared with standard of care. Similarly, they reported fewer nonclient partners in the direct delivery arm (3.19, 95% CI 5.18 to 1.21, P ¼ 0.002) and in the coupon arm (1.84, 95% CI 3.81 to 0.14, P ¼ 0.07) arm compared with standard of care. Conclusion: Expansion of HIV self-testing may have positive behavioral effects enhancing other HIV prevention efforts among female sex workers in Zambia. Trial Registration: ClinicalTrials.gov NCT02827240. Copyrigh