Proline-rich antimicrobial peptide, PR-39 gene transduction altered invasive activity and actin structure in human hepatocellular carcinoma cells

PR-39 is an endogenous proline-rich antimicrobial peptide which induces the synthesis of syndecan-1, a transmembrane heparan sulphate proteoglycan involved in cell-to-matrix interactions and wound healing. Previously, we revealed that the expression of syndecan-1 was reduced in human hepatocellular carcinomas with high metastatic potential and speculated that syndecan-1 played an important role in inhibition of invasion and metastasis. It is assumed that a modification of this process with PR-39 and syndecan-1 may result in a new strategy by which it can inhibit the invasion and metastasis. Therefore, we transduced a gene of PR-39 into human hepatocellular carcinoma cell line HLF, which shows a low expression of syndecan-1 and a high in vitro invasive activity, and examined whether this procedure could reduce the invasive activity of tumour cells. In two transfectants with PR-39 gene, the syndecan-1 expression was induced and the invasive activity in type I collagen-coated chamber was inhibited. Moreover, these transfectants showed the suppression of motile activity assayed by phagokinetic tracks in addition to the disorganization of actin filaments observed by a confocal imaging system. In contrast, five transfectants with syndecan-1 gene in the HLF cells revealed suppression of invasive activity but did not alter the motile activity and actin structures of the cell. These results suggest that PR-39 has functions involved in the suppression of motile activity and alteration of actin structure on human hepatocellular carcinoma cells in addition to the suppression of invasive activity which might result from the induction of syndecan-1 expression. © 1999 Cancer Research Campaig

On obtaining optimal well rates and placement for CO2 storage

Large-scale storage of CO2 in saline aquifers is considered an essential technology to mitigate CO2 emissions. Storage potential has mainly been estimated based on volumetrics or detailed simulations for specific injection scenarios. In practice, achievable storage capacity will depend on engineering, economical, and political restrictions and be limited by the length of the injection period, costs associated with potential CO2 leakage, pressure management, etc. We show how achievable storage volumes can be estimated and maximized using adjoint-based optimization and a hierarchy of simulation methods. In particular, vertical equilibrium models provide the simplest possible description of the flow dynamics during the injection and early post-injection period, while percolation type methods provide effective means for forecasting the long-term fate of CO2 during the later migration stages. We investigate the storage volumes that can be achieved for several formations found along the Norwegian Continental Shelf by optimizing well placement and injection rates, and using production wells for pressure management when necessary. Optimal strategies are obtained under various objectives and simple but realistic constraints, namely: penalization of CO2 leakage, minimization of well cost, and restriction of pressure buildup.acceptedVersio