Changes in growth, survival and digestive enzyme activities of Asian redtail catfish, Mystus nemurus, larvae fed on different diets

A study was conducted to determine the effects of different dietary treatments on the growth, survival and digestive enzyme activities of Mystus nemurus larvae. Newly hatched larvae were reared for 14 days in twelve 15 L glass aquaria (for growth and survival) and eight 300 L fiberglass tanks (for enzyme samples) at a stocking density of 15 larvae L-1. Beginning at 2 days, the larvae were randomly assigned to Artemia nauplii, a microbound diet and a 50:50 combination of live food-microbound diet, while another group was unfed. All treatments were triplicated (growth and survival) or duplicated (enzyme development). The results showed that, M. nemurus larvae which fed on Artemia nauplii gave the highest survival rate (83.7%), followed by those fed on a combination diet (56.0%) and a microbound diet (26.5%). All unfed larvae did not survive beyond Day 9. Artemia had also given the best growth (20.4 ± 1.4 mm TL and 37.2 ± 6.0 mg wet weight) for the catfish larvae. This was followed by the combination diet (18.3 ± 0.6 mm TL and 32.6 ± 3.4 mg wet weight) and the microbound diet (11.0 ± 0.1 mm TL and 11.9 ± 0.9 mg wet weight), respectively. Pepsin began to significantly appear in M. nemurus larvae at 4 days old for all treatments, while chymotrypsin, trypsin and amylase were present even in the newly hatched larvae. In general, highest enzyme activities were observed among larvae which fed on a combination diet, followed by those fed on live and artificial diets, respectively. This suggested the important role of exogenous enzymes from live food in the larval digestion particularly at the early feeding stages.Key words: Mystus nemurus, Artemia nauplii, larvae, microbound diet, combination diet

Hybridization And Sexual Reproduction In The Invasive Alien Fallopia (Polygonaceae) Complex In Belgium

peer reviewed† Background and Aims The knotweed complex, Fallopia spp. (Polygonaceae), belongs to the most troublesome invasive species in Europe and North America. Vegetative regeneration is widely recognized as the main mode of reproduction in the adventive regions. However, the contribution of sexual reproduction to the success of these invasive species has only been detailed for the British Isles. An examination was made as to how hybridization may influence the sexual reproduction of the complex in Belgium and to determine how it may contribute to the dispersal of the species. † Methods Studies were made of floral biology, reproductive success, seed rain, seed bank, germination capacity, seedling survival and dispersal capacity in order to characterize the reproductive biology of the species. Moreover, chromosome counts and flow cytometry were used to assess the hybrid status of seedlings produced by sexual reproduction. † Key Results In the area investigated, extensive sexual reproduction by hybridization within the complex, including one horticultural species, was demonstrated. A small percentage of seeds may be dispersed outside the maternal clone (.16 m) allowing the formation of genetically differentiated individuals. Seed germination was possible even after a winter cold period. †Conclusions The extensive sexual reproduction by hybridization could further contribute to the dramatic invasive success of knotweeds in Belgium and should not be underestimated when considering control and management measures

Immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (Bubalus bubalis) calves orally exposed to Pasteurella multocida B:2

The gastrointestinal lesions and bacterial distribution of buffalo calves were evaluated histologically using immunoperoxidase, following oral exposure to wild-type Pasteurella multocida B:2 at 109cfu/mL in   phosphate buffered saline. The lesions were basically of mild to severe mucohaemorrhagic abomasitis and  enteritis.The lesions were confirmed to be associated with the inoculated P. multocida B:2, using the   immunoperoxidase technique. P. multocida B:2 antigen was detected not only in the bacterial clusters in  the gastric pits, intestinal epithelia and capillaries, Brünner’s glands and Crypt of Lieberkühn but was also  seen interacting with infiltrating neutrophils and macrophages intracellularly and on the surface of  erythrocyte in congested vessels and haemorrhages. We observed higher localization and distribution of the  immunoperoxidase reaction with increased severity of lesions along the gastrointestinal tract. This suggest intensity increases with increased amount of P. multocida B:2 or antigen in the tissue, which  possibly leads to increase tissue damage.Keywords: Buffalo calves, Gastrointestinal lesions, Immunoperoxidase, Oral infection, Pasteurella  multocida B:

An Electro-Optical Technique For The Measurement Of Temperature Of High Potential Bodies.

In this paper we have presented a low cost, simple and robust mechatronic system for the measurement of temperature in extreme conditions. It is specially designed for the protection of high potential parts of power transformers and switch gears under faulty operating conditions

Public Integrity Auditing for Dynamic Data Sharing With Multiuser Modification

In cloud storage systems, information proprietors have their information on cloud servers furthermore, clients (information customers) can get to the information from cloud servers. Because of the information outsourcing, be that as it may, this new worldview of information facilitating administration additionally presents new security challenges, which requires an autonomous evaluating administration to check the information honesty in the cloud. In huge scale distributed storage frameworks, the information might be refreshed powerfully, so existing remote uprightness checking strategies served for static chronicle information are no longer appropriate to check the information uprightness. Accordingly, a proficient and secure dynamic inspecting convention is wanted to persuade information proprietors that the information is accurately put away in the cloud. In this section, we initially present an evaluating structure for cloud capacity frameworks. At that point, we depict Third-party Auditing Scheme a proficient and security saving evaluating convention for distributed storage, which can likewise bolster information dynamic operations and cluster reviewing for both various proprietors what's more

Evaluation of in vitro Vibrio static activity of Shewanella algae isolated from healthy Penaeus monodon

To conquer disease problem in shrimp industries, probiotic biocontrol is a well known remedy now. The antagonistic ability of separated isolates from different parts of juvenile Penaeus monodon werescreened against shrimp Vibrio pathogens; Vibrio parahaemolyticus and Vibrio alginolyticus. The most antagonistic effect was observed for an isolate that was primarily identified as Shewanella algae usingconventional methods followed by Biolog microlog software. Since production of antagonistic agents rely on cultural conditions, antagonistic ability of candidate probioic against the mentioned Vibrios was assessed using Response Surface Methodology, with central composite design in which four independents variables were assumed: temperature (10 - 50°C), pH (6 -10), NaCl concentration (0 - 50%)and time (12 – 60 h). The coefficients of multiple determinations (R2), for the responses of antagonistic effect of S. algae against V. parahaemolyticus and V. alginolyticus values were 0.807 and 0.805,respectively. Concentration of the NaCl exhibited least influence on the antibacterial effect of candidate probiotic while the other independent variables exhibited different degree of affect. The candidateprobiotic revealed a reasonable antibacterial response in quite a wide range of temperature and pH in which the maximum levels were in the same range of optimum shrimp culture

Optimization of admixture and three-layer particleboard made from oil palm empty fruit bunch and rubberwood clones

Empty fruit bunch (EFB) is a biomass that is widely available and has the potential to be used as industrial raw material especially in wood-based industries. This study focuses on producing a particleboard by incorporating EFB with two different rubberwood clones: Prang Besar (PB) 260 and RRIM 2002, respectively. PB 260 is a commercially planted clone and wood from matured (>25 year-old) trees are used by wood-based panel manufacturers. RRIM 2002 is a new clone planted at the Malaysian Rubber Board (MRB) research trial plots and consists of only 4-year-old trees. Two types of particleboards (admixture and three-layer) with different ratios were produced. The Japanese Industrial Standard (JIS-5908 2003 particleboard) was used to evaluate mechanical and dimensional stability properties of the particleboards. From the study, it was found that admixture particleboards showed superior properties compared to three-layer particleboards. Layering EFB and rubberwood significantly decreased board performance for all properties (except internal bonding). The optimum ratios of EFB and both rubberwood clones are found to be 1:1 (50% EFB: 50% rubberwood). Meanwhile, increasing the rubberwood clones ratio to 70% lowered board performance especially for EFB (30%):RRIM 2002 clone (70%) boards which showed the lowest values for all properties for both admixture and three-layer board

The (A)gamma-195 (C -> G) mutation in hereditary persistence of fetal hemoglobin is not associated with activation of a reporter gene in vitro

Hereditary persistence, of fetal hemoglobin is an uncommon, benign disorder in which the expression of gamma -globin genes persists into adult life. Several point mutations have been associated with the increased gamma -globin gene promoter activity. We evaluated the -195 (C-->G) mutation by a functional in vitro assay based on the luciferase reporter gene system. The results indicated that the increased promoter activity observed in vivo could not be reproduced in vitro, under the conditions employed, suggesting that other factors may be involved in the overexpression of the gamma -globin gene containing the -195 (C-->G) mutation. Furthermore: this is the first time that the -195 (C-->G) mutation of the (A)gamma -globin gene has been evaluated by in vitro gene expression.34448949

Optimizing Clinical Benefits of Bisphosphonates in Cancer Patients with Bone Metastases

Malignant bone disease is common in patients with advanced solid tumors or multiple myeloma. Bisphosphonates have been found to be important treatments for bone metastases. A positive benefit-risk ratio for bisphosphonates has been established, and ongoing clinical trials will determine whether individualized therapy is possible