Hepatic Failure: Role for biochemists and nutrition experts

Nutritional support to hepatic failure patients is challenging and requires experience, skill, careful planning and meticulous follow-up. It is indeed an attempt to replenish the lost power of one of the most vital organs we possess

Laboratory Generated Artifacts in Plasma Amino acid Quantitation

The pace of physicians’ involvement in amino acid metabolism has been enormous in the last five decades. With further development of technology to identify and quantitate upto picomoles of amino acids, their metabolites and related peptides, diagnosis and effective medical intervention in cases of inherited metabolic disorders have been well within the reach of the clinician. Automatic amino acid analyzers have become an essential part of major medical and research centers around the world. The technology has come indeed as a boon to physicians who in particular deal with inherited defects of amino acid metabolism. However, the technology comes with the risk of major deviations from the actual results when a few minor variations are not looked into. Trivial variations in basic steps of obtaining the sample, the choice of anticoagulant, hemolysis etc. can cause significant variations in the resulting values, particularly while dealing with inherited defects of amino acid metabolism and their treatment/management. Effects of such factors are revisited here for the benefit of the modern day laboratory personnel

Gene Therapy – Potential, Pros, Cons and Ethics

Genetic technology poses risks along with its rewards, just as any technology has in the past. To stop its development and forfeit the benefits gene therapy could offer would be a far greater mistake than forging ahead could ever be. People must always try to be responsible with their new technology, but gene therapy has the potential to be the future of medicine and its possibilities must be explored

Chromatin remodeling during mammalian spermatogenesis: role of testis specific histone variants and transition proteins.

The structure of chromatin undergoes extensive alteration during mammalian spermatogenesis. Several testis specific histone subtypes are synthesised and replace their somatic counterparts during pre-meiotic, meiotic and post-meiotic stages of germ cell differentiation. Early work from our laboratory showed that pachytene spermatocyte nuclei as well as nucleosome core particle are more accessible to DNasel than the interphase liver nuclei. The higher order structure of chromatin in pachytene spermatocytes is also loosely packed due to the poor DNA and chromatin condensing property of the testis specific linker histone H1t. A careful analysis of the amino acid sequence of histone H1t revealed the absence of the DNA condensing domain containing SPKK/TPKK motifs in the C-terminus of the histone H1t. The spermiogenesis process following the meiotic division is characterised by extensive remodeling of chromatin. Transition proteins, TP1 and TP2, unique to mammalian spermatogenesis play an important role in this spermiogenesis process. We have shown that TP1 is a DNA melting protein while TP2 is a DNA condensing protein. We have delineated the molecular anatomy of TP2 including the presence of two novel zinc finger modules, which are essential for the recognition of CpG islands in the genome. TP2 is also phosphorylated by sperm specific protein kinase A and the phosphorylation/dephosphorylation cycle plays an important role in the chromatin condensation process

Assessment of Metabolic Parameters For Autism Spectrum Disorders

Autism is a brain development disorder that first appears during infancy or childhood, and generally follows a steady course without remission. Impairments result from maturation-related changes in various systems of the brain. Autism is one of the five pervasive developmental disorders (PDD), which are characterized by widespread abnormalities of social interactions and communication, and severely restricted interests and highly repetitive behavior. The reported incidence of autism spectrum disorders (ASDs) has increased markedly over the past decade. The Centre for Disease Control and Prevention has recently estimated the prevalence of ASDs in the United States at approximately 5.6 per 1000 (1 of 155 to 1 of 160) children. Several metabolic defects, such as phenylketonuria, are associated with autistic symptoms. In deciding upon the appropriate evaluation scheme a clinician must consider a host of different factors. The guidelines in this article have been developed to assist the clinician in the consideration of these factors

Customer retention through customer satisfaction In relation to Pioneer Honda, Ongole.

The phrase "customer satisfaction" is commonly used in marketing. It evaluates how well a company's goods and services meet or surpass those of its clients. "The number of customers, or percentage of total customers, whose reported experience with a firm, its products, or its services (ratings) exceeds specified satisfaction goals." Customer satisfaction (abbreviated CSAT) is a marketing term that is frequently used. It is a measure of how well a company's products and services meet or exceed customer expectations. "The number of customers, or percentage of total customers, whose reported experience with a firm, its products, or its services (ratings) exceeds specified satisfaction goals." As part of its ongoing Common Language in Marketing Project, the Marketing Accountability Standards Board (MASB) endorses the definitions, purposes, and constructs of classes of measures found in Marketing Metrics. In a survey of nearly 200 senior marketing executives, 71% said a customer satisfaction metric was very useful in managing and monitoring their businesses

A NOVEL TECHNIQUE FOR SECURE ENCRYPTED MESSAGES IN MOBILE AND PERVASIVE APPLICATIONS

More than applications rely on the existence of small devices that can exchange information and form communication networks. In a significant portion of such applications, the confidentiality and integrity of the communicated messages are of particular interest. In this work, to propose two novel techniques for authenticating short encrypted messages that are directed to meet the requirements of mobile and pervasive applications. By taking advantage of the fact that the message to be authenticated must also be encrypted, to propose provably secure authentication codes that are more efficient than any message authentication code in the literature. The key idea behind the proposed techniques is to utilize the security that the encryption algorithm can provide to design more efficient authentication mechanisms, as opposed to using standalone authentication primitives

Self Controllable Health Care Monitoring Arrangement for Patient

In this undertaking is utilized to the Condition care monitoring system. Distributed Healthcare cloud computing arrangement considerably facilitates effectual patient treatment for health consultation by allocating confidential condition data amid healthcare providers. Though, it brings concerning the trial of keeping both the data confidentiality and patients’ individuality privacy simultaneously. Countless continuing admission manipulation and nameless authentication schemes cannot be straightforwardly exploited. The arrangement acts there are provider, doctor, patient and admin. The provider is list to website to consent staying to appeal dispatch to admin. Admin is Proved to in a particular provider it deeds to the present add to doctors and hospital divisions established. User or Patient is list to the site. Patient Login to present the deed booking the doctor appointment in situation patient to dispatch a feedback to that doctor treatment comments onward to admin. Doctors is add provider to dispatch a username and password .Doctor is login to think patient appointment features and checking the doctor is present patient or fake user to identified to dispatch to symptoms description upload files(x-ray).Admin is finished procedure is upheld in this system. Patient dispatch doctors feedback bad or wrong to particular doctors appointment annulled temporally. In this undertaking generally utilized for patient and hospital ,doctors features through online upheld for India astute established on card

Studies on the effect of doxorubicin on MDA, NO2, NO3, Se-GSH peroxidase and SOD levels in albino rat tissues

Doxorubicin, a highly effective anticancer drug, produces cardiotoxicity, which limits its therapeutic potential. The mechanism of this cardiotoxicity has remained elusive. The use of this drug, however,continues to be limited by its dose-related and time interval toxicity. Reactive oxygen species are hypothesized to be a major factor in the toxicity of doxorubicin. The aim of this work was to investigate theeffect of doxorubicin on dose-related and time interval in rat. The study utilized adult albino rats (120 ± 5 g). They were divided into 8 groups of 7 animals each and were kept under standard laboratory conditions.They had free access to commercial pellet diet and water. The room temperature was maintained at 20 ± 5°C. The study measured rat tissue MDA, NO2, NO3, Se-GSH peroxidase and SOD under Dox stress, and the results indicate that MDA and NO generated in Dox treated samples cause neuro, myo, hepato and renal toxicity. Since SOD and Peroxidase are scavenging molecules, increase in their levels in Dox treated samples may be one of mechanism to overcome Dox caused oxidative stress in Dox treated albino rat