Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing

Pterygium is a lesion of the eye surface which involves cell proliferation, migration, angiogenesis, fibrosis, and extracellular matrix remodelling. Surgery is the only approved method to treat this disorder, but high recurrence rates are common. Recently, it has been shown in a mouse model that treatment with doxycycline resulted in reduction of the pterygium lesions. Here we study the mechanism(s) of action by which doxycycline achieves these results, using massive sequencing techniques. Surgically removed pterygia from 10 consecutive patients were set in short term culture and exposed to 0 (control), 50, 200, and 500 µg/ml doxycycline for 24 h, their mRNA was purified, reverse transcribed and sequenced through Illumina’s massive sequencing protocols. Acquired data were subjected to quantile normalization and analyzed using cytoscape plugin software to explore the pathways involved. False discovery rate (FDR) methods were used to identify 332 genes which modified their expression in a dose-dependent manner upon exposure to doxycycline. The more represented cellular pathways included all mitochondrial genes, the endoplasmic reticulum stress response, integrins and extracellular matrix components, and growth factors. A high correlation was obtained when comparing ultrasequencing data with qRT-PCR and ELISA results

Endoscopic decompression of orbit and optic nerve in Graves’ disease: Case report

Grave’s disease is a clinical syndrome consisting of hypermetabolism, diffuse thyroid enlargement and exophthalmos. Which has ocular complications. Due to the high incidence of these complications and the risk of blindness special attention is needed. Many therapeutic methods including medical surgical and radiotherapy for ocular complications are suggested. In cases of medical therapy failure, surgery is recommended. One of its methods is orbital decompression and if needed optic nerve decompression by transnasal endoscopy. A Graves’ patient with bilateral exophthalmos and severe visual loss was operated. With this method. After surgery in addition to return of normal eye appearance and eye movements, visual acuity of left eye improved significantly. This article describes this method, its results and complications

Frequency of Eyelid Malpositions and their Treatment Results in Patients Referred to Ophthalmology Clinics in Educational Yazd Hospitals

Introduction: Entropion and ectropion with involutional changes are the most common eyelid malpositions. Entropion is a condition in which the eyelid margin turns inwards against the globe and ectropion is a malposition in which the lid turns outwards or is pulled away its normal position from the globe. Knowledge of eyelid problems and their abnormalities is a key to plan a successful surgical procedure. Methods: This descriptive study lasted from Sep. 2008 to March 2009. We evaluated 78 patients with eyelid malpositions who referred to educational hospitals of Yazd. The patients were examined by an eye specialist. Each patient’s demographic data, type of eyelid malposition, specific treatment results and 6-month follow up of each patient were recorded by the questioners and then were analyzed statistically by SPSS13 software, chi-square and fisher exact test. Results: Among 78 cases, entropion was observed in 41 cases(52.6%), trichiasis in 31 cases(39.7%), ectropion in 27 cases(34.6%) and distichiasis in 3 cases (3.8%). The Wies procedure proved to be influential in entropion treatment and lateral tarsal strip canthoplasty surgery also reported acceptable results in ectropion. Conclusion: The involutional changes were the most common causing factors for eyelid malpositions. Entropion appears most prevalently specially in right upper eyelid. The Wies and lateral tarsal strip canthoplasty procedures were thoroughly successful in treatment of entropion and ectropion of either upper or lower eyelid

Benign Eyelid Lesions: Six Months Study

Introduction: Benign eyelid lesions are classified to infectious, inflammatory and tumoral lesions. The various number of these lesions is due to unique eyelid anatomical structure. Some lesions are simple and do not need any treatment but sometimes patients refer to ophthalmologists due to beauty or problems such as pain, swelling. The aim of our study is to investigate and compare the relative frequency of benign eyelid lesions in Shahid Sadoughi eye clinic as well as the private offices. Methods: This cross sectional six month study was done on 247 patients who referred to Shahid Sadoughi eye clinic and the private offices in 2011. They were examined by direct observation and slit lamp regardless of the cause. The information was gleaned by a questionnaire and the research data was analyzed by SPSS (ver.16). Results: we studied 247 patients including 84(34%) male and 163(6%) female). The mean age of the patients was 42.7 years. 60.2% of lesions were in upper lid and 39.8% were in lower lid. 51.2% of lesions were in right eye and 48.8% were in left eye. The most common eyelid lesions were papilloma (32.9%) nevus (21.9%) and chalazion (14.1%) respectively. Conclusion: Papilloma, nevus and chalazion are respectively the most common lesion in men and women. Benign lesions were more common in women located more in upper lid, though there was no statistically difference between right and left lid