Aprendizajes de Estudiantes de Enfermería Involucrados en Proyectos de Investigación: Revisión Integrativa de la Literatura

To identify the learning outcomes and skills obtained of undergraduate nursing students involved in research projects. Methods: This was an integrative literature review, based on a research protocol in the CINAHL Complete databases; Cochrane Central Register of Controlled Trials; Cochrane Database of Systematic Reviews; Cochrane Methodology Register; MedicLatina; MEDLINE, Scopus and JBI, including primary and secondary studies, published between 2015 and 2020. Results: A total of five heterogeneous articles were included, which were categorized using Kirkpatrick’s (adapted) model. Seventeen learning outcomes acquired through participation in research projects were identified, from the learning of new knowledge and skills to the development of new attitudes and behaviors. Final considerations: The involvement of nursing students in research projects is important to their professional development. Future investment in research on this topic can help cement the potential of this type of student involvement.info:eu-repo/semantics/publishedVersio

Intersetorialidade e potenciais conflitos de interesse entre governos e setor privado comercial no âmbito das ações de alimentação e nutrição para o enfrentamento de doenças crônicas não transmissíveis | Intersectoriality and potential conflicts of interest between governments and private, commercial sector within the context of food and nutrition actions to deal with chronic non-communicable diseases

Neste artigo foram analisados os potenciais conflitos entre interesses governamentais e privados de caráter comercial em torno das ações de alimentação e nutrição (AN) no âmbito de propostas intersetoriais de enfrentamento das DCNT no Brasil. Para tal, foram identificados artigos acadêmicos e documentos institucionais publicados a partir de 2000 que abordassem as ações de AN em políticas de DCNT pautadas pela intersetorialidade. Foram priorizados elementos que contribuíssem para a compreensão dos potenciais conflitos de interesse entre governos e o setor privado comercial no âmbito de estratégias intersetoriais de enfrentamento de DCNT. Concluiu-se que o princípio da intersetorialidade, desassociado de um processo político que problematize os critérios de eleição e as práticas institucionais dos setores que respondem pelo conjunto de ações que compõe as políticas governamentais, pode servir como facilitador do favorecimento de interesses comerciais. A participação no processo decisório de setores de interesse comercial cujas práticas ferem objetivos e princípios das políticas públicas, movidos pela expansão da demanda por seus produtos e pela concentração progressiva de poder, pode retardar, atenuar ou impedir ações efetivas na redução de DCNT. Esta possibilidade é particularmente relevante no âmbito de atribuições que são próprias aos entes Estatais, especialmente as estratégias regulatórias. ------------------------------------------------------------------------------------------- This article analyzed the potential conflicts of interest between government and private commercial sectors in the area of food and nutrition (FN) activities within the context of intersectoral proposals for dealing with chronic non-communicable diseases (CNCD) in Brazil. To this end, academic articles and institutional documents published after 2000 were identified that addressed FN actions in CNCD policies guided by intersectoriality. Elements that contributed to understanding the potential conflicts of interest between governments and the private, commercial sector within the context of intersectoral strategies to deal with CNCD were prioritized. It was concluded that the principle of intersectoriality disassociated from a political process that complicates the eligibility criteria and institutional practices of the sectors responsible for the set of actions that comprise government policies may serve as facilitators for favoring commercial interests. Participation in the decision-making process of the sectors of commercial interest whose practices, driven by increased demand for their products and the progressive concentration of power, contradict the purposes and principles of public policy, may delay, reduce, or prevent effective actions to reduce CNCD. This possibility is particularly relevant in the context of attributions that are specific to government entities, especially regulatory strategies

Intersetorialidade e potenciais conflitos de interesse entre governos e setor privado comercial no âmbito das ações de alimentação e nutrição para o enfrentamento de doenças crônicas não transmissíveis

This article analyzed the potential conflicts of interest between government and private commercial sectors in the area of food and nutrition (FN) activities within the context of intersectoral proposals for dealing with chronic non-communicable diseases (CNCD) in Brazil. To this end, academic articles and institutional documents published after 2000 were identified that addressed FN actions in CNCD policies guided by intersectoriality. Elements that contributed to understanding the potential conflicts of interest between governments and the private, commercial sector within the context of intersectoral strategies to deal with CNCD were prioritized. It was concluded that the principle of intersectoriality disassociated from a political process that complicates the eligibility criteria and institutional practices of the sectors responsible for the set of actions that comprise government policies may serve as facilitators for favoring commercial interests. Participation in the decision-making process of the sectors of commercial interest whose practices, driven by increased demand for their products and the progressive concentration of power, contradict the purposes and principles of public policy, may delay, reduce, or prevent effective actions to reduce CNCD. This possibility is particularly relevant in the context of attributions that are specific to government entities, especially regulatory strategies.Neste artigo foram analisados os potenciais conflitos entre interesses governamentais e privados de caráter comercial em torno das ações de alimentação e nutrição (AN) no âmbito de propostas intersetoriais de enfrentamento das DCNT no Brasil. Para tal, foram identificados artigos acadêmicos e documentos institucionais publicados a partir de 2000 que abordassem as ações de AN em políticas de DCNT pautadas pela intersetorialidade. Foram priorizados elementos que contribuíssem para a compreensão dos potenciais conflitos de interesse entre governos e o setor privado comercial no âmbito de estratégias intersetoriais de enfrentamento de DCNT. Concluiu-se que o princípio da intersetorialidade, desassociado de um processo político que problematize os critérios de eleição e as práticas institucionais dos setores que respondem pelo conjunto de ações que compõe as políticas governamentais, pode servir como facilitador do favorecimento de interesses comerciais. A participação no processo decisório de setores de interesse comercial cujas práticas ferem objetivos e princípios das políticas públicas, movidos pela expansão da demanda por seus produtos e pela concentração progressiva de poder, pode retardar, atenuar ou impedir ações efetivas na redução de DCNT. Esta possibilidade é particularmente relevante no âmbito de atribuições que são próprias aos entes Estatais, especialmente as estratégias regulatórias

Identification and functional characterization of a novel arginine/ornithine transporter, a member of a cationic amino acid transporter subfamily in the Trypanosoma cruzi genome

Background: Trypanosoma cruzi, the etiological agent of Chagas disease, is auxotrophic for arginine. It obtains this amino acid from the host through transporters expressed on the plasma membrane and on the membranes of intracellular compartments. A few cationic amino acid transporters have been characterized at the molecular level, such as the novel intracellular arginine/ornithine transporter, TcCAT1.1, a member of the TcCAT subfamily that is composed of four almost identical open reading frames in the T. cruzi genome. Methods: The functional characterization of the TcCAT1.1 isoform was performed in two heterologous expression systems. TcCAT subfamily expression was evaluated by real-time PCR in polysomal RNA fractions, and the cellular localization of TcCAT1.1 fused to EGFP was performed by confocal and immunoelectron microscopy. Results: In the S. cerevisiae expression system, TcCAT1.1 showed high affinity for arginine (Km = 0.085 ± 0.04 mM) and low affinity for ornithine (Km = 1.7 ± 0.2 mM). Xenopus laevis oocytes expressing TcCAT1.1 showed a 7-fold increase in arginine uptake when they were pre-loaded with arginine, indicating that transport is enhanced by substrates on the trans side of the membrane (trans-stimulation). Oocytes that were pre-loaded with [3H]-arginine displayed a 16-fold higher efflux of [3H]-arginine compared with that of the control. Analysis of polysomal RNA fractions demonstrated that the expression of members of the arginine transporter TcCAT subfamily is upregulated under nutritional stress and that this upregulation precedes metacyclogenesis. To investigate the cellular localization of the transporter, EGFP was fused to TcCAT1.1, and fluorescence microscopy and immunocytochemistry revealed the intracellular labeling of vesicles in the anterior region, in a network of tubules and vesicles. Conclusions: TcCAT1.1 is a novel arginine/ornithine transporter, an exchanger expressed in intracellular compartments that is physiologically involved in arginine homeostasis throughout the T. cruzi life cycle. The properties and estimated kinetic parameters of TcCAT1.1 can be extended to other members of the TcCAT subfamily

A DNA repair-independent role for alkyladenine DNA glycosylase in alkylation-induced unfolded protein response.

Alkylating agents damage DNA and proteins and are widely used in cancer chemotherapy. While cellular responses to alkylation-induced DNA damage have been explored, knowledge of how alkylation affects global cellular stress responses is sparse. Here, we examined the effects of the alkylating agent methylmethane sulfonate (MMS) on gene expression in mouse liver, using mice deficient in alkyladenine DNA glycosylase (Aag), the enzyme that initiates the repair of alkylated DNA bases. MMS induced a robust transcriptional response in wild-type liver that included markers of the endoplasmic reticulum (ER) stress/unfolded protein response (UPR) known to be controlled by XBP1, a key UPR effector. Importantly, this response is significantly reduced in the Aag knockout. To investigate how AAG affects alkylation-induced UPR, the expression of UPR markers after MMS treatment was interrogated in human glioblastoma cells expressing different AAG levels. Alkylation induced the UPR in cells expressing AAG; conversely, AAG knockdown compromised UPR induction and led to a defect in XBP1 activation. To verify the requirements for the DNA repair activity of AAG in this response, AAG knockdown cells were complemented with wild-type Aag or with an Aag variant producing a glycosylase-deficient AAG protein. As expected, the glycosylase-defective Aag does not fully protect AAG knockdown cells against MMS-induced cytotoxicity. Remarkably, however, alkylation-induced XBP1 activation is fully complemented by the catalytically inactive AAG enzyme. This work establishes that, besides its enzymatic activity, AAG has noncanonical functions in alkylation-induced UPR that contribute to cellular responses to alkylation

Comparison of Methods to Correct Survival Estimates and Survival Regression Analysis on a Large HIV African Cohort

The evaluation of HIV treatment programs is generally based on an estimation of survival among patients receiving antiretroviral treatment (ART). In large HIV programs, loss to follow-up (LFU) rates remain high despite active patient tracing, which is likely to bias survival estimates and survival regression analyses

Single-molecule imaging to characterise the transport mechanism of the Nuclear Pore Complex

In the eukaryotic cell, a large macromolecular channel, known as the Nuclear Pore Complex (NPC), mediates all molecular transport between the nucleus and cytoplasm. In recent years, single-molecule fluorescence (SMF) imaging has emerged as a powerful tool to study the molecular mechanism of transport through the NPC. More recently, techniques such as Single-Molecule Localisation Microscopy (SMLM) have enabled the spatial and temporal distribution of cargos, transport receptors and even structural components of the NPC to be determined with nanometre accuracy. In this protocol, we describe a method to study the position and/or motion of individual molecules transiting through the NPC with high spatial and temporal precision

Observation of associated near-side and away-side long-range correlations in √sNN=5.02 TeV proton-lead collisions with the ATLAS detector

Two-particle correlations in relative azimuthal angle (Δϕ) and pseudorapidity (Δη) are measured in √sNN=5.02  TeV p+Pb collisions using the ATLAS detector at the LHC. The measurements are performed using approximately 1  μb-1 of data as a function of transverse momentum (pT) and the transverse energy (ΣETPb) summed over 3.1<η<4.9 in the direction of the Pb beam. The correlation function, constructed from charged particles, exhibits a long-range (2<|Δη|<5) “near-side” (Δϕ∼0) correlation that grows rapidly with increasing ΣETPb. A long-range “away-side” (Δϕ∼π) correlation, obtained by subtracting the expected contributions from recoiling dijets and other sources estimated using events with small ΣETPb, is found to match the near-side correlation in magnitude, shape (in Δη and Δϕ) and ΣETPb dependence. The resultant Δϕ correlation is approximately symmetric about π/2, and is consistent with a dominant cos⁡2Δϕ modulation for all ΣETPb ranges and particle pT