A Type 2C Protein Phosphatase FgPtc3 Is Involved in Cell Wall Integrity, Lipid Metabolism, and Virulence in Fusarium graminearum

Type 2C protein phosphatases (PP2Cs) play important roles in regulating many biological processes in eukaryotes. Currently, little is known about functions of PP2Cs in filamentous fungi. The causal agent of wheat head blight, Fusarium graminearum, contains seven putative PP2C genes, FgPTC1, -3, -5, -5R, -6, -7 and -7R. In order to investigate roles of these PP2Cs, we constructed deletion mutants for all seven PP2C genes in this study. The FgPTC3 deletion mutant (ΔFgPtc3-8) exhibited reduced aerial hyphae formation and deoxynivalenol (DON) production, but increased production of conidia. The mutant showed increased resistance to osmotic stress and cell wall-damaging agents on potato dextrose agar plates. Pathogencity assays showed that ΔFgPtc3-8 is unable to infect flowering wheat head. All of the defects were restored when ΔFgPtc3-8 was complemented with the wild-type FgPTC3 gene. Additionally, the FgPTC3 partially rescued growth defect of a yeast PTC1 deletion mutant under various stress conditions. Ultrastructural and histochemical analyses showed that conidia of ΔFgPtc3-8 contained an unusually high number of large lipid droplets. Furthermore, the mutant accumulated a higher basal level of glycerol than the wild-type progenitor. Quantitative real-time PCR assays showed that basal expression of FgOS2, FgSLT2 and FgMKK1 in the mutant was significantly higher than that in the wild-type strain. Serial analysis of gene expression in ΔFgPtc3-8 revealed that FgPTC3 is associated with various metabolic pathways. In contrast to the FgPTC3 mutant, the deletion mutants of FgPTC1, FgPTC5, FgPTC5R, FgPTC6, FgPTC7 or FgPTC7R did not show aberrant phenotypic features when grown on PDA medium or inoculated on wheat head. These results indicate FgPtc3 is the key PP2C that plays a critical role in a variety of cellular and biological functions, including cell wall integrity, lipid and secondary metabolisms, and virulence in F. graminearum

Excess-entropy scaling in supercooled binary mixtures

Supercooled liquids near the glass transition show remarkable non-Arrhenius transport phenomena, whose origin is yet to be clarified. Here, the authors use GPU molecular dynamics simulations for various binary mixtures in the supercooled regime to show the validity of a quasiuniversal excess-entropy scaling relation for viscosity and diffusion

Diurnal circulations and their multi-scale interaction leading to rainfall over the South China Sea upstream of the Philippines during intraseasonal monsoon westerly wind bursts

The morning diurnal precipitation maximum over the coastal sea upstream of the Philippines during intraseasonal westerly wind bursts is examined from observations and numerical model simulations. A well-defined case of precipitation and large-scale circulation over the coastal sea west of the Philippines during 17-27 June 2004 is selected as a representative case. The hypothesis is that the mesoscale diurnal circulation over the Philippines and a large-scale diurnal circulation that is induced by large-scale differential heating over Asian continent and the surrounding ocean interact to produce the offshore precipitation maximum during the morning. Three-hourly combined satellite microwave and infrared rainfall retrievals define the morning rainfall peak during this period, and then later the stratiform rain area extends toward the open sea. A control numerical simulation in which a grid-nudging four-dimensional data assimilation (FDDA) is applied to force the large-scale diurnal circulation represents reasonably well the morning rainfall maximum. An enhanced low-level convergence similar to observations is simulated due to the interaction of the local- and large-scale diurnal circulations. The essential role of the local-scale diurnal circulation is illustrated in a sensitivity test in which the solar zenith angle is fixed at 7 am to suppress this diurnal circulation. The implication for climate diagnosis or modeling of such upstream coastal sea precipitation maxima is that the diurnal variations of both the local- and the large-scale circulations must be taken into considerationclose6

Anomalous Decoupling of Translational and Rotation Motion Under 1D Confinement, Evidences from Crystallization and Diffusion Experiments

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