Genotypes and Toxin Gene Profiles of Staphylococcus aureus Clinical Isolates from China

A total of 108 S. aureus isolates from 16 major hospitals located in 14 different provinces in China were characterized for the profiles of 18 staphylococcal enterotoxin (SE) genes, 3 exfoliatin genes (eta, etb and etd), and the toxic shock syndrome toxin gene (tsst) by PCR. The genomic diversity of each isolate was also evaluated by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and accessory gene regulator (agr) typing. Of these strains, 90.7% (98/108) harbored toxin genes, in which tsst was the most prevalent toxin gene (48.1%), followed by sea (44.4%), sek (42.6%) and seq (40.7%). The see and etb genes were not found in any of the isolates tested. Because of high-frequency transfer of toxin gene-containing mobile genetic elements between S. aureus strains, a total of 47 different toxin gene combinations were detected, including a complete egc cluster in 19 isolates, co-occurrence of sea, sek and seq in 38 strains, and sec and sel together in 11 strains. Genetic typing by PFGE grouped all the strains into 25 clusters based on 80% similarity. MLST revealed 25 sequence types (ST) which were assigned into 16 clonal complexes (CCs) including 2 new singletons. Among these, 11 new and 6 known STs were first reported in the S. aureus strains from China. Overall, the genotyping results showed high genetic diversity of the strains regardless of their geographical distributions, and no strong correlation between genetic background and toxin genotypes of the strains. For genotyping S. aureus, PFGE appears to be more discriminatory than MLST. However, toxin gene typing combined with PFGE or MLST could increase the discriminatory power of genotyping S. aureus strains

Self-healing materials and structures for geotechnical and geo-environmental applications

The nature-inspired concept of self-healing materials in construction is relatively new and has recently attracted significant attention as this could bring about substantial savings in maintenance costs as well as enhance the durability and serviceability and improve the safety of our structures and infrastructure. Much of the research and applications to date has focused on concrete, for structural applications, and on asphalt, with significant advances being made. However, to date no attention has been given to the incorporation of self- healing concepts in geotechnical and geo-environmental applications. This includes the use of concrete and other stabilising agents in foundations and other geotechnical structures, grouts, grouted soil systems, soil-cement systems and slurry walls for ground improvement and land remediation applications. The recently established Materials for Life (M4L) project funded by EPSRC has initiated research activities in the UK focussing on those applications. The project involves the development and integration of the use of microcapsules, biological agents, shape memory polymers and vascular networks as healing systems. The authors are exploring development of self-healing systems using mineral admixtures, microencapsulation and bio-cementation applications. The paper presents an overview of those initiatives to date and potential applications and presents some relevant preliminary results