P- and S- wave velocities of consolidated sediments from a seafloor seismic survey in the North Celtic Sea Basin, offshore Ireland

A geophysical survey was conducted over a hydrocarbon prospect in the North Celtic Sea Basin using a small array of ocean-bottom seismographs (OBSs). The purpose of this study was to determine the ratio of (P) compressional- to (S) shear-wave velocity of consolidated sedimentary rocks in order to constrain possible subsurface variations in pore-fluid content. The ratio of VP and VS- is known to be particularly sensitive to lithology, porosity and pore-fluid content, making it a useful parameter for evaluating hydrocarbon prospects. OBSs offer a relatively cheap and time-effective means of acquiring multi-component data compared with ocean-bottom cables. In this contribution, we demonstrate the ability of an OBS survey comprising three pairs of two OBSs spaced at 1.6 km to recover lateral variations in the VP/VS ratio. A key requirement of this type of study is that S-waves will be generated by mode conversions in the subsurface, since they cannot be generated in nor travel through fluids. In this survey, the contrast in physical properties of the hard seabed of the North Celtic Sea Basin provided a means of generating converted S-waves. Two-dimensional ray-tracing and forward modeling was used to create both VP and VS models along a profile crossing the Blackrock prospect in the North Celtic Sea Basin. These models comprise four layers and extend to a maximum depth of 1.1 km. The observed northward decrease in the VP/VS ratio at depths of 500-1000 m below the seafloor in the study area is interpreted to represent lateral variation in the amount of gas present in the pore space of Upper Cretaceous chalks and shales overlying the prospective reservoir

Morphology and tectonics of the Mid-Atlantic Ridge, 7°–12°S

We present swath bathymetric, gravity, and magnetic data from the Mid-Atlantic Ridge between the Ascension and the Bode Verde fracture zones, where significant ridge–hot spot interaction has been inferred. The ridge axis in this region may be divided into four segments. The central two segments exhibit rifted axial highs, while the northernmost and southernmost segments have deep rift valleys typical of slow-spreading mid-ocean ridges. Bathymetric and magnetic data indicate that both central segments have experienced ridge jumps since ~1 Ma. Mantle Bouguer anomalies (MBAs) derived from shipboard free air gravity and swath bathymetric data show deep subcircular lows centered on the new ridge axes, suggesting that mantle flow has been established beneath the new spreading centers for at least ~1 Myr. Inversion of gravity data indicates that crustal thicknesses vary by ~4 km along axis, with the thickest crust occurring beneath a large axial volcanic edifice. Once the effects of lithospheric aging have been removed, a model in which gravity variations are attributed entirely to crustal thickness variations is more consistent with data from an axis-parallel seismic line than a model that includes additional along-axis variations in mantle temperature. Both geophysical and geochemical data from the region may be explained by the melting of small (<200 km) mantle chemical heterogeneities rather than elevated temperatures. Therefore, there may be no Ascension/Circe plume

Analysis of histone post translational modifications in primary monocyte derived macrophages using reverse phase×reverse phase chromatography in conjunction with porous graphitic carbon stationary phase.

A two dimensional-liquid chromatography (2D-LC) based approach was developed for the identification and quantification of histone post translational modifications in conjunction with mass spectrometry analysis. Using a bottom-up strategy, offline 2D-LC was developed using reverse phase chromatography. A porous graphitic carbon stationary phase in the first dimension and a C18 stationary phase in the second dimension interfaced with mass spectrometry was used to analyse global levels of histone post translational modifications in human primary monocyte-derived macrophages. The results demonstrated that 84 different histone peptide proteoforms, with modifications at 18 different sites including combinatorial marks were identified, representing an increase in the identification of histone peptides by 65% and 51% compared to two different 1D-LC approaches on the same mass spectrometer. The use of the porous graphitic stationary phase in the first dimension resulted in efficient separation of histone peptides across the gradient, with good resolution and is orthogonal to the online C18 reverse phase chromatography. Overall, more histone peptides were identified using the 2D-LC approach compared to conventional 1D-LC approaches. In addition, a bioinformatic pipeline was developed in-house to enable the high throughput efficient and accurate quantification of fractionated histone peptides. The automation of a section of the downstream analysis pipeline increased the throughput of the 2D-LC-MS/MS approach for the quantification of histone post translational modifications

Cenozoic evolution of the eastern Black Sea: a test of depth-dependent stretching models

Subsidence analysis of the eastern Black Sea basin suggests that the stratigraphy of this deep, extensional basin can be explained by a predominantly pure-shear stretching history. A strain-rate inversion method that assumes pure-shear extension obtains good fits between observed and predicted stratigraphy. A relatively pure-shear strain distribution is also obtained when a strain-rate inversion algorithm is applied that allows extension to vary with depth without assuming its existence or form. The timing of opening of the eastern Black Sea, which occupied a back-arc position during the closure of the Tethys Ocean, has also been a subject of intense debate; competing theories called for basin opening during the Jurassic, Cretaceous or Paleocene/Eocene. Our work suggests that extension likely continued into the early Cenozoic, in agreement with stratigraphic relationships onshore and with estimates for the timing of arc magmatism. Further basin deepening also appears to have occurred in the last 20 myr. This anomalous subsidence event is focused in the northern part of the basin and reaches its peak at 15–10 Ma. We suggest that this comparatively localized shortening is associated with the northward movement of the Arabian plate. We also explore the effects of paleowater depth and elastic thickness on the results. These parameters are controversial, particularly for deep-water basins and margins, but their estimation is a necessary step in any analysis of the tectonic subsidence record stored in stratigraphy. <br/

Fault and magmatic interaction within Iceland's western rift over the last 9kyr

We present high-resolution 'Chirp' sub-bottom profiler data from Thingvallavatn, a lake in Iceland's western rift zone. These data are combined with stratigraphic constraints from sediment cores to show that movement on normal faults since 9 ka are temporally correlated with magmatic events, indicating that movements were controlled by episodic dyke intrusion. Sediment depo-centres and the focus of subsidence migrated westwards over 3-4 kyr towards the locus of subsequent brittle failure. We interpret this subsidence as related to dyke intrusion a few km along strike, originating from the Hengill volcanic system, which occurred prior to major dyking, faulting and subsidence within the lake at 1.9 ka

Knee joint neuromuscular activation performance during muscle damage and superimposed fatigue

This study examined the concurrent effects of exercise-induced muscle damage and superimposed acute fatigue on the neuromuscular activation performance of the knee flexors of nine males (age: 26.7 ± 6.1yrs; height 1.81 ± 0.05m; body mass 81.2 ± 11.7kg [mean ± SD]). Measures were obtained during three experimental conditions: (i) FAT-EEVID, involving acute fatiguing exercise performed on each assessment occasion plus a single episode of eccentric exercise performed on the first occasion and after the fatigue trial; (ii) FAT, involving the fatiguing exercise only and; (iii) CON consisting of no exercise. Assessments were performed prior to (pre) and at lh, 24h, 48h, 72h, and 168h relative to the eccentric exercise. Repeated-measures ANOVAs showed that muscle damage within the FAT-EEVID condition elicited reductions of up to 38%, 24%) and 65%> in volitional peak force, electromechanical delay and rate of force development compared to baseline and controls, respectively (F[io, 80] = 2.3 to 4.6; p to 30.7%>) following acute fatigue (Fp; i6] = 4.3 to 9.1; p ; Fp, iq = 3.9; p <0.05). The safeguarding of evoked muscle activation capability despite compromised volitional performance might reveal aspects of capabilities for emergency and protective responses during episodes of fatigue and antecedent muscle damaging exercise

A family of thermostable fungal cellulases created by structure-guided recombination

SCHEMA structure-guided recombination of 3 fungal class II cellobiohydrolases (CBH II cellulases) has yielded a collection of highly thermostable CBH II chimeras. Twenty-three of 48 genes sampled from the 6,561 possible chimeric sequences were secreted by the Saccharomyces cerevisiae heterologous host in catalytically active form. Five of these chimeras have half-lives of thermal inactivation at 63°C that are greater than the most stable parent, CBH II enzyme from the thermophilic fungus Humicola insolens, which suggests that this chimera collection contains hundreds of highly stable cellulases. Twenty-five new sequences were designed based on mathematical modeling of the thermostabilities for the first set of chimeras. Ten of these sequences were expressed in active form; all 10 retained more activity than H. insolens CBH II after incubation at 63°C. The total of 15 validated thermostable CBH II enzymes have high sequence diversity, differing from their closest natural homologs at up to 63 amino acid positions. Selected purified thermostable chimeras hydrolyzed phosphoric acid swollen cellulose at temperatures 7 to 15°C higher than the parent enzymes. These chimeras also hydrolyzed as much or more cellulose than the parent CBH II enzymes in long-time cellulose hydrolysis assays and had pH/activity profiles as broad, or broader than, the parent enzymes. Generating this group of diverse, thermostable fungal CBH II chimeras is the first step in building an inventory of stable cellulases from which optimized enzyme mixtures for biomass conversion can be formulated

The role of cyclin synthesis, modification and destruction in the control of cell division

This paper reviews our current knowledge of the cyclins based on observations of the oocytes and eggs of sea urchins, clams and frogs. Cyclins are proteins found in all eukaryotes whose special property is rapid destruction at specific stages in the cell cycle. The cyclins fall into three families. A-type cyclins have been found in clams, flies and frogs. B-type cyclins have been found in clams, flies, frogs, sea urchins and fission yeast. A more distantly related family of three genes is found in Saccharomyces cerevisiae. B-type cyclins appear to be required for cells to enter mitosis, and their destruction is thought to be necessary for exit from mitosis. We describe evidence in support of these ideas, and describe various conditions under which cyclin destruction is delayed or deranged. We conclude with a discussion of the relationship between the cyclins and maturation- (or M phase-) promoting factor and some ideas on how the cyclins may work

Resolving the fine-scale velocity structure of continental hyperextension at the Deep Galicia Margin using full-waveform inversion

Continental hyperextension during magma-poor rifting at the Deep Galicia Margin is characterised by a complex pattern of faulting, thin continental fault blocks, and the serpentinisation, with local exhumation, of mantle peridotites along the S-reflector, interpreted as a detachment surface. In order to understand fully the evolution of these features, it is important to image seismically the structure and to model the velocity structure to the greatest resolution possible. Travel-time tomography models have revealed the long-wavelength velocity structure of this hyperextended domain, but are often insufficient to match accurately the short-wavelength structure observed in reflection seismic imaging. Here we demonstrate the application of two-dimensional (2D) time-domain acoustic full-waveform inversion to deep water seismic data collected at the Deep Galicia Margin, in order to attain a high resolution velocity model of continental hyperextension. We have used several quality assurance procedures to assess the velocity model, including comparison of the observed and modelled waveforms, checkerboard tests, testing of parameter and inversion strategy, and comparison with the migrated reflection image. Our final model exhibits an increase in the resolution of subsurface velocities, with particular improvement observed in the westernmost continental fault blocks, with a clear rotation of the velocity field to match steeply dipping reflectors. Across the S-reflector there is a sharpening in the velocity contrast, with lower velocities beneath S indicative of preferential mantle serpentinisation. This study supports the hypothesis that normal faulting acts to hydrate the upper mantle peridotite, observed as a systematic decrease in seismic velocities, consistent with increased serpentinisation. Our results confirm the feasibility of applying the full-waveform inversion method to sparse, deep water crustal datasets